PEPD (NM_000285) Human Untagged Clone

CAT#: SC119982

PEPD (untagged)-Human peptidase D (PEPD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEPD
• Synonyms: PROLIDASE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000285, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTG
  CCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGT
  CCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTC
  TTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGA
  CCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTT
  CAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG
  CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTG
  ACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAA
  GACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATG
  AAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGG
  GCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGG
  ACACGCCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG
  TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGG
  CCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGGTGGCC
  TGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGC
  GTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCA
  TTGACGTGCACGACGTGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCT
  GCGCACTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC
  CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTC
  GCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTGACCTG
  CGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCT
  GGCCCCAAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000285 unedited
  ATTTTTCANATTTTGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTGA
  CGCCGGTGCCGGGCGAACTGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAA
  CCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGC
  GGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTC
  AGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGT
  TCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCC
  TGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGG
  AGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCG
  TCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCG
  GCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCA
  TTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTC
  TGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAG
  TGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCNGGNGCG
  GCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGACNTCAGCCGTGCTAC
  ACTACGGACACGCCGGAGCTNCCAACGACCGAACGATTCAGAATGGGGATATGTGCCCTG
  TTCGACATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000285 unedited
  TTGGACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGACCATTTTAGGAAA
  CCTTTTATTGCAAATGCCATTCTGCATATTGATTTTTGACAGAAAGTATCAGAAATGCTT
  CTTTCCTGGGAAAAGGAATATAAATGACAGCAAGACACATTTTAGTTGCTACTAAAGAAC
  AGCATTATTTTCAATCATTTTAAGTCGCTCATTTAATAAGCAAGGTATAAAACAGATTAA
  AGGTGGGAGCCTGCAAAAGGGTAATTAAAAAAGTGTTTCCTCCCCGGGAAACAGCACTGT
  TTGGTCTGATCAAATGCCGAAGCTGGGATCTGATTCTGGGTGCCGTCTCTCGCTACTGGA
  GTGCTGACCAGCAGGCTGCCCATCACGAAAAGAGGTTGCAAGGCCAGGCCCCCAGGTGCG
  CTGGGATTTCTGGCTGGCTCTACTTGGGGCCAGAGAAGGGGGTAAAGGCCTTGTCACAGC
  CTGCCATGCATGCTTCAATCTCTTCCACAGTGCGGGGCACGCAGGTCAGCAGCTCTATGC
  CGCTGTCAGTCACCACGACGTCCTCCTCGATGCGGACCCCGCCAAAACCGCGAAAGCGCT
  GCAGGACCTCGCGGTTAAGGAAGGAGGCGCGGGCCGGGTCCGCCAGGGCCTCATCCAGGA
  GGTGGTCGATGAAGTAGATGCCCGGCTCCACAGTGAGCACCATGCCTGGCTGCANGTGCC
  GTGCANTGCGCANGCTCCGCAGGCCCGGCTCGTCGATGCGCTCCACGCCCTCTGGGTAGC
  CTCCCCCTCATGCCCGTAATGCCAGGAAGTGGCCAACCCGTGAAGCATAAACCCGCGCCC
  AGGGAACCCTGACCCTGGCGTTCACCCTGCCTCAAGATGGCCATGTGGGCCAACTCCTTC
  AAGGGGAGCGGTAANCCAGCCGGGCTGTAAGGCCCCCAACCCTGGCTTTTGCCCCCCTGA
  CGGCCGGAGCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000285
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000285.1, NP_000276.1
• RefSeq Size: 1888 bp
• RefSeq ORF: 1482 bp
• Locus ID: 5184
• Cytogenetics: 19q13.11
• Domains: Peptidase_M24, AMP_N
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).