PEPD (NM_000285) Human Untagged Clone

CAT#: SC322376

PEPD (untagged)-Human peptidase D (PEPD), transcript variant 1


  "NM_000285" in other vectors (5)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PEPD
Synonyms PROLIDASE
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322376 GCCGCACTTCACGTGACGCCGGTGCCGGGCGAACATGGCGGCGGCCACCGGACCCTCGTT
TTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGGCAGCG
CCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTCCTGCA
GGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTC
CTTCTTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGA
CACTGGGAAGTCGACCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGG
AAAGATCCATTCCAAGGAGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGT
AGATGAGATTGCCAGCGTCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTGCGTGG
CGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGCAAGTT
CGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAGACGGA
TATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGT
AATGAAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTA
CTGCTACTCCCGGGGCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGA
GAACTCAGCCGTGCTACACTACGGACACGCCGGAGCTCCCAACGACCGAACGATCCAGAA
TGGGGATATGTGCCTGTTCGACATGGGCGGTGAGTATTACTGCTTCGCTTCCGACATCAC
CTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGGCCGTCTATGAGGCAGT
GCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGGTGGCCTGACAT
GCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGCATCCTGAGCGG
CAGCGTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCTCACGGGCTTGG
CCACTTCCTGGGCATTGACGTGCACGACGTGGGAGGCTACCCAGAGGGCGTGGAGCGCAT
CGACGAGCCCGGCCTGCGGAGCCTGCGCACTGCACGGCACCTGCAGCCAGGCATGGTGCT
CACCGTGGAGCCGGGCATCTACTTCATCGACCACCTCCTGGATGAGGCCCTGGCGGACCC
GGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTCGCGGTTTTGGCGGGGT
CCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTGACCTGCGTGCC
CCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCCTTTACCCCCTT
CTCTGGCCCCAAGTAGAGCCAGCCAGAAATCCCAGCGCACCTGGGGGCCTGGCCTTGCAA
CCTCTTTTCGTGATGGGCAGCCTGCTGGTCAGCACTCCAGTAGCGAGAGACGGCACCCAG
AATCAGATCCCAGCTTCGGCATTTGATCAGACCAAACAGTGCTGTTTCCCGGGGAGGAAA
CACTTTTTTAATTACCCTTTTGCAGGCTCCCACCTTTAATCTGTTTTATACCTTGCTTAT
TAAATGAGCGACTTAAAATGATTGAAAATAATGCTGTTCTTTAGTAGCAACTAAAATGTG
TCTTGCTGTCATTTATATTCCTTTTCCCAGGAAAGAAGCATTTCTGATACTTTCTGTCAA
AAATCAATATGCAGAATGGCATTTGCAATAAAAGGTTTCCTAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000285
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000285.1, NP_000276.1
RefSeq Size 1888 bp
RefSeq ORF 1482 bp
Locus ID 5184
Cytogenetics 19q13.11
Domains Peptidase_M24, AMP_N
Protein Families Druggable Genome, Protease
Gene Summary 'This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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