PEX12 (NM_000286) Human Untagged Clone

CAT#: SC119983

PEX12 (untagged)-Human peroxisomal biogenesis factor 12 (PEX12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PEX12
• Synonyms: PAF-3; PBD3A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000286, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGCACGGGGCTCACTTCACAGCTGCTTCTGTGGCCGATGACCAGCCATCCATCTTTGAGGTGG
  TAGCACAGGACAGTTTAATGACAGCAGTGAGACCCGCTCTTCAGCATGTGGTCAAGGTTCTTGCAGAATC
  AAATCCCACCCACTATGGCTTCTTGTGGAGGTGGTTTGATGAAATCTTTACTCTGCTAGATCTTCTGCTC
  CAGCAACATTATCTGTCTAGAACCAGTGCCTCATTTTCTGAAAACTTTTACGGCTTAAAGAGAATTGTAA
  TGGGGGACACTCACAAGTCTCAGAGATTGGCTAGTGCTGGTCTCCCAAAGCAGCAGCTTTGGAAATCTAT
  TATGTTCCTGGTTCTTCTTCCCTATCTGAAAGTGAAGCTGGAGAAGCTGGTTTCTAGCCTGAGAGAAGAG
  GATGAATATTCTATTCATCCCCCTTCTTCCCGCTGGAAACGATTTTACAGAGCTTTCCTGGCAGCCTACC
  CATTTGTGAACATGGCCTGGGAAGGATGGTTTCTTGTACAACAACTTCGATACATCCTAGGAAAAGCTCA
  GCATCACTCACCACTGCTGAGGCTGGCTGGAGTTCAGCTAGGTCGACTGACAGTTCAGGATATACAAGCT
  CTGGAGCACAAACCAGCTAAGGCCAGCATGATGCAGCAACCAGCCAGGAGTGTTAGTGAGAAGATAAACT
  CAGCTCTGAAGAAAGCTGTTGGGGGTGTTGCCTTATCCCTGTCTACTGGCCTTTCTGTGGGTGTATTCTT
  CTTGCAGTTCCTTGACTGGTGGTACTCATCTGAAAATCAAGAAACCATCAAGTCATTGACTGCCCTGCCT
  ACTCCACCACCACCTGTACACCTAGACTATAACTCTGATTCTCCCCTCTTACCCAAAATGAAGACTGTGT
  GCCCACTGTGTCGTAAAACCCGGGTGAATGATACTGTTCTTGCCACCTCTGGCTATGTGTTTTGTTACCG
  CTGTGTGTTTCATTATGTGAGGAGTCACCAAGCTTGTCCCATCACAGGTTATCCAACAGAAGTACAACAT
  CTGATTAAACTCTACTCCCCTGAGAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000286 unedited
  TCAAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGCAAGGAC
  GGGCGCGGGCGGGGATACTCAAAGCCAACAGCTGGAGTCAGCCCTCGTGTCCCGGGCTCA
  CAGTGGCACGACTGAATCCTCAGAGTCGGCTGGCTTTTGAGCTCTCACGATTGGGGAGGA
  GGGGGCGTTTCTGGTTCGCAGCTCCAGAGGATTGCGTTCCTTCCCCCATACCTGTCCCCC
  ACAGTCACGCTCTGCCCTGACGTGCAGCATTTGACAAGTTACCCCCTCGCCACATACTAC
  TTCCACCCACGTCCGAGTTAACTTTGTTCTTAACCTTCTTGAGACTACCCTCGGCCTCCA
  GGTCTTTTTTTCCCAGTTCATTTTTGCCCATAAGATTGAGTTTCGAGTTTCAGATATCAT
  GCAGAAAGTTTACCTTTAAGACTGAGCACCCATCTGATACTCTTCCTCCCGAAAAAGTTC
  ATGCTCACGAGAGAGTTTGTGGGAAAAGTGAAAGCCAGTACACGCAGGAAACTATGGCTG
  AGCACGGGGCTCACTTCACAGCTGCTTCTGTGGCCGATGACCAGCCATCCATCTTTGAGG
  TGGTAGCACAGGACAGTTTAATGACAGCAGTGAGACCCGCTCTTCAGCATGTGGTCAAGG
  TTCTTGCAGAATCAAATCCCACCCACTATGGCTTCTTGTGGAGGTGGTTTGATGAAATCT
  TTACTCTGCTAGATCTTCTGCTCCAGCACATTATCTGTCTAGACCAGTGCCTCATTTTCT
  GAAAACTTTTACGGCTTANAGAGATTGTATGGGGGACACTCACAGTCTCGAGATGGGCTG
  TGCNTGTCTCCCAAGCACAGCTTTGAATCTATATGTCCTGGNTCTCTTCCCTACTGAAGT
  GAGCTNGAGAGCTGNTTCTACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000286 unedited
  AACGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTCTGAATGAAAAAT
  TATTTTAAACACCTCAAGTAATCTTTACATTTCTACAAAATCATAACATAGTTCTTTTCC
  AGAAACATTTATTCTTAAAATCTACTTGTTTCATAGAAACTTTAGTAATAACCAGAATAA
  TTCATGACACTTTAGCAAACTCTTAGCAGCAAGCAGAAAGAACTTATTAAAATATCCTTT
  CTTTACACAATTAGATTAGTATTTTGCTTCATGTCTTCATTGCTTGTTAGCTTTGCTTTT
  ACACAGGAATCTTCAGACTACTCTCTGTAACACTCAACCCCTATTCCTTTTTTAACCAGT
  TTCTCAAGAAGGATATGGAGAGCTCATAAAGGAATATCCTCAAATATCCTAAATATCCAA
  CTTTTAAAATACCTTTTTTATCCTTAAAAGCTGATAAACAATAGATGTCACTTAACAAAT
  GTCTCACAGTATATGGAAGGAGCTTTTTCCCCCCCCAGAGTGTTTCTAAGCATTTCTATC
  AAGAGCAAGTCTGCATTTCTTATCGTTTGCAAACTGATGAAATTTCCACCTTACAGACAG
  GCAGGTAAATGCTTTCTTTTCTAAAAAGAAGTCTTGCTACCACCTCACTGTTCACTTTAT
  TTTTTATGCGGTCTAACCTGGGTTTTTCGTAAGAGCTCCTTTATTTCTTATATTTTAAGG
  AAAGGGATAGGCAAAAACTTGGGTACTCATTATTCTGTTTAATCTCTACACTGGCCCTCA
  TATCCCCTGCCAGTCCTGATCCTTCTACTTTAAGCAGCTTGGTCAACTGTNAGTAGGGTT
  CTAGAGAGCAGGCTAAAAGGTTAGGATCAAATGGGCATATCCTTTTAAAGAAACCATATA
  ATCATTTAAGGTCATATAGNTATGAATTGAGGCTGAGAATGTCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000286
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000286.1, NP_000277.1
• RefSeq Size: 2609 bp
• RefSeq ORF: 1080 bp
• Locus ID: 5193
• Cytogenetics: 17q12
• Domains: Pex2_Pex12
• Gene Summary: 'This gene belongs to the peroxin-12 family. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of Zellweger syndrome (ZWS). [provided by RefSeq, Oct 2008]'