MSH6 (NM_000179) Human Untagged Clone

CAT#: SC120035

MSH6 (untagged)-Human mutS homolog 6 (E. coli) (MSH6)


  "NM_000179" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MSH6
Synonyms GTBP; GTMBP; HNPCC5; HSAP; p160
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120035 sequence for NM_000179 edited (data generated by NextGen Sequencing)
ATGTCGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCC
AACAAGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCC
TCTCCTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTG
GCGCGCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTA
GCGCCTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATG
GAGGGTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATC
CGCGAGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGG
GGCTGGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAG
AAGGGAGGTCATTTTTACAGTGCAAAGCCTGAAATACTGAGAGCAATGCAACGTGCAGAT
GAAGCCTTAAATAAAGACAAGATTAAGAGGCTTGAATTGGCAGTTTGTGATGAGCCCTCA
GAGCCAGAAGAGGAAGAAGAGATGGAGGTAGGCACAACTTACGTAACAGATAAGAGTGAA
GAAGATAATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAAGGATCTAGGCGA
AGTAGCCGCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATTGGTGGC
TCTGATGTGGAATTTAAGCCAGACACTAAGGAGGAAGGAAGCAGTGATGAAATAAGCAGT
GGAGTGGGGGATAGTGAGAGTGAAGGCCTGAACAGCCCTGTCAAAGTTGCTCGAAAGCGG
AAGAGAATGGTGACTGGAAATGGCTCTCTTAAAAGGAAAAGCTCTAGGAAGGAAACGCCC
TCAGCCACCAAACAAGCAACTAGCATTTCATCAGAAACCAAGAATACTTTGAGAGCTTTC
TCTGCCCCTCAAAATTCTGAATCCCAAGCCCACGTTAGTGGAGGTGGTGATGACAGTAGT
CGCCCTACTGTTTGGTATCATGAAACTTTAGAATGGCTTAAGGAGGAAAAGAGAAGAGAT
GAGCACAGGAGGAGGCCTGATCACCCCGATTTTGATGCATCTACACTCTATGTGCCTGAG
GATTTCCTCAATTCTTGTACTCCTGGGATGAGGAAGTGGTGGCAGATTAAGTCTCAGAAC
TTTGATCTTGTCATCTGTTACAAGGTGGGGAAATTTTATGAGCTGTACCACATGGATGCT
CTTATTGGAGTCAGTGAACTGGGGCTGGTATTCATGAAAGGCAACTGGGCCCATTCTGGC
TTTCCTGAAATTGCATTTGGCCGTTATTCAGATTCCCTGGTGCAGAAGGGCTATAAAGTA
GCACGAGTGGAACAGACTGAGACTCCAGAAATGATGGAGGCACGATGTAGAAAGATGGCA
CATATATCCAAGTATGATAGAGTGGTGAGGAGGGAGATCTGTAGGATCATTACCAAGGGT
ACACAGACTTACAGTGTGCTGGAAGGTGATCCCTCTGAGAACTACAGTAAGTATCTTCTT
AGCCTCAAAGAAAAAGAGGAAGATTCTTCTGGCCATACTCGTGCATATGGTGTGTGCTTT
GTTGATACTTCACTGGGAAAGTTTTTCATAGGTCAGTTTTCAGATGATCGCCATTGTTCG
AGATTTAGGACTCTAGTGGCACACTATCCCCCAGTACAAGTTTTATTTGAAAAAGGAAAT
CTCTCAAAGGAAACTAAAACAATTCTAAAGAGTTCATTGTCCTGTTCTCTTCAGGAAGGT
CTGATACCCGGCTCCCAGTTTTGGGATGCATCCAAAACTTTGAGAACTCTCCTTGAGGAA
GAATATTTTAGGGAAAAGCTAAGTGATGGCATTGGGGTGATGTTACCCCAGGTGCTTAAA
GGTATGACTTCAGAGTCTGATTCCATTGGGTTGACACCAGGAGAGAAAAGTGAATTGGCC
CTCTCTGCTCTAGGTGGTTGTGTCTTCTACCTCAAAAAATGCCTTATTGATCAGGAGCTT
TTATCAATGGCTAATTTTGAAGAATATATTCCCTTGGATTCTGACACAGTCAGCACTACA
AGATCTGGTGCTATCTTCACCAAAGCCTATCAACGAATGGTGCTAGATGCAGTGACATTA
AACAACTTGGAGATTTTTCTGAATGGAACAAATGGTTCTACTGAAGGAACCCTACTAGAG
AGGGTTGATACTTGCCATACTCCTTTTGGTAAGCGGCTCCTAAAGCAATGGCTTTGTGCC
CCACTCTGTAACCATTATGCTATTAATGATCGTCTAGATGCCATAGAAGACCTCATGGTT
GTGCCTGACAAAATCTCCGAAGTTGTAGAGCTTCTAAAGAAGCTTCCAGATCTTGAGAGG
CTACTCAGTAAAATTCATAATGTTGGGTCTCCCCTGAAGAGTCAGAACCACCCAGACAGC
AGGGCTATAATGTATGAAGAAACTACATACAGCAAGAAGAAGATTATTGATTTTCTTTCT
GCTCTGGAAGGATTCAAAGTAATGTGTAAAATTATAGGGATCATGGAAGAAGTTGCTGAT
GGTTTTAAGTCTAAAATCCTTAAGCAGGTCATCTCTCTGCAGACAAAAAATCCTGAAGGT
CGTTTTCCTGATTTGACTGTAGAATTGAACCGATGGGATACAGCCTTTGACCATGAAAAG
GCTCGAAAGACTGGACTTATTACTCCCAAAGCAGGCTTTGACTCTGATTATGACCAAGCT
CTTGCTGACATAAGAGAAAATGAACAGAGCCTCCTGGAATACCTAGAGAAACAGCGCAAC
AGAATTGGCTGTAGGACCATAGTCTATTGGGGGATTGGTAGGAACCGTTACCAGCTGGAA
ATTCCTGAGAATTTCACCACTCGCAATTTGCCAGAAGAATACGAGTTGAAATCTACCAAG
AAGGGCTGTAAACGATACTGGACCAAAACTATTGAAAAGAAGTTGGCTAATCTCATAAAT
GCTGAAGAACGGAGGGATGTATCATTGAAGGACTGCATGCGGCGACTGTTCTATAACTTT
GATAAAAATTACAAGGACTGGCAGTCTGCTGTAGAGTGTATCGCAGTGTTGGATGTTTTA
CTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGGTCCTATGTGTCGCCCAGTAATTCTG
TTGCCGGAAGATACCCCCCCCTTCTTAGAGCTTAAAGGATCACGCCATCCTTGCATTACG
AAGACTTTTTTTGGAGATGATTTTATTCCTAATGACATTCTAATAGGCTGTGAGGAAGAG
GAGCAGGAAAATGGCAAAGCCTATTGTGTGCTTGTTACTGGACCAAATATGGGGGGCAAG
TCTACGCTTATGAGACAGGCTGGCTTATTAGCTGTAATGGCCCAGATGGGTTGTTACGTC
CCTGCTGAAGTGTGCAGGCTCACACCAATTGATAGAGTGTTTACTAGACTTGGTGCCTCA
GACAGAATAATGTCAGGTGAAAGTACATTTTTTGTTGAATTAAGTGAAACTGCCAGCATA
CTCATGCATGCAACAGCACATTCTCTGGTGCTTGTGGATGAATTAGGAAGAGGTACTGCA
ACATTTGATGGGACGGCAATAGCAAATGCAGTTGTTAAAGAACTTGCTGAGACTATAAAA
TGTCGTACATTATTTTCAACTCACTACCATTCATTAGTAGAAGATTATTCTCAAAATGTT
GCTGTGCGCCTAGGACATATGGCATGCATGGTAGAAAATGAATGTGAAGACCCCAGCCAG
GAGACTATTACGTTCCTCTATAAATTCATTAAGGGAGCTTGTCCTAAAAGCTATGGCTTT
AATGCAGCAAGGCTTGCTAATCTCCCAGAGGAAGTTATTCAAAAGGGACATAGAAAAGCA
AGAGAATTTGAGAAGATGAATCAGTCACTACGATTATTTCGGGAAGTTTGCCTGGCTAGT
GAAAGGTCAACTGTAGATGCTGAAGCTGTCCATAAATTGCTGACTTTGATTAAGGAATTA
TAG

Clone variation with respect to NM_000179.2
>OriGene 5' read for NM_000179 unedited
TTGGATTTTGTAACCGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGCGCGGTAGAT
GCGGTGCTTTTAGGAGCTCCGTCCGACAGAACGGTTGGGCCTTGCCGGCTGTCGGTATGT
CGCGACAGAGCACCCTGTACAGCTTCTTCCCCAAGTCTCCGGCGCTGAGTGATGCCAACA
AGGCCTCGGCCAGGGCCTCACGCGAAGGCGGCCGTGCCGCCGCTGCCCCCGGGGCCTCTC
CTTCCCCAGGCGGGGATGCGGCCTGGAGCGAGGCTGGGCCTGGGCCCAGGCCCTTGGCGC
GCTCCGCGTCACCGCCCAAGGCGAAGAACCTCAACGGAGGGCTGCGGAGATCGGTAGCGC
CTGCTGCCCCCACCAGTTGTGACTTCTCACCAGGAGATTTGGTTTGGGCCAAGATGGAGG
GTTACCCCTGGTGGCCTTGTCTGGTTTACAACCACCCCTTTGATGGAACATTCATCCGCG
AGAAAGGGAAATCAGTCCGTGTTCATGTACAGTTTTTTGATGACAGCCCAACAAGGGGCT
GGGTTAGCAAAAGGCTTTTAAAGCCATATACAGGTTCAAAATCAAAGGAAGCCCAGAAGG
GAGGTCATTTTTACAGTGCANAGCCTGANATACTGAGAGCAATGCAACGTGCAGATGAAG
CCTTAATAAAGACAGATTAAGAGGCTTNGATTGGCAGTTTGTGATGAGCCCTCAGAGCCA
GAAGAGGAAGAAGAGATGGANGTAGGGCACACTTTACGTACAGATAAGAGTGAAGAAAGA
TATGAAATTGAGAGTGAAGAGGAAGTACAGCCTAAGACACAGGGATCTAGCGAAGTAGCC
GCCAAATAAAAAAACGAAGGGTCATATCAGATTCTGAGAGTGACATGGNTGGCTCTGATG
TGGAATTTAAGCCCAGAACCTAAGGAGGAAAGAN
>OriGene 3' read for NM_000179 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTTTTAAAAAATAAAG
TTTATTAGATCATAATGTTGTCTGAATTTACCACCTTTGTCAGAAGTCAACTCAAAGCTT
CCAATGTAGTCAGTCTATAATTCCTTAATCAAAGTCAGCAATTTATGGACAGCTTCAGCA
TCTACAGTTGACCTTTCACTAGCCAGGCAAACTTCCCGAAATAATCGTAGTGACTGATTC
ATCTTCTCAAATTCTCTTGCTTTTCTATGTCCCTTTTGAATAACTTCCTCTGGGAGATTA
GCAAGCCTTGCTGCATTAAAGCCATAGCTTTTAGGACAAGCTCCCTTAATGAATTTATAG
AGGAACGTAATAGTCTCCTGGCTGGGGTCTTCACATTCATTTTCTACCATGCATGCCATA
TGTCCTAGGCGCACAGCAACATTTTGAGAATAATCTTCTACTAATGAATGGTAGTGAGTT
GAAAATAATGTACGACATTTTATAGTCTCAGCAAGTTCTTTAACAACTGCATTTGCTATT
GCCGTCCCATCAAATGTTGCAGTACCTCTTCCTAATTCATCCACAAGCACCAGAGAATGT
GCTGTTGCATGCATGAGTATGCTGGCAGTTTCACTTAATTCAACAAAAAATGTACTTTCA
CCTGACATTATTCTGTCTGAGGCACCAAGTCTAGTAAACACTCTATCAATTGGTGTGAGC
CTGCACACTTCAGCAGGGACGTAACAACCCATCTGGGCCATTACAGCTAATAAGCCAGCC
TGTCTCATAAGCGTAGACTTGCCCCNCATATTTGGTCCAGTAACAAGCACACATAGGGCT
TGCCAATTTCCTGCTCCTCTTCTCACAGNCTATTAGAATGTCATTAGGATAAAATCATCT
CCAAAAAAGTCTTGTATGCAGGATGCGGGATCTTTAGCT
Restriction Sites NotI-NotI     
ACCN NM_000179
Insert Size 4310 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000179.1, NP_000170.1
RefSeq Size 4264 bp
RefSeq ORF 4083 bp
Locus ID 2956
Cytogenetics 2p16.3
Domains PWWP, MutS_V, MutS_I, MutS_III, MutS_II, MutS_IV
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Colorectal cancer, Mismatch repair, Pathways in cancer
Gene Summary This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1).

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