TBX5 (NM_000192) Human Untagged Clone

CAT#: SC120046

TBX5 (untagged)-Human T-box 5 (TBX5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX5
• Synonyms: HOS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120046 sequence for NM_000192 edited (data generated by NextGen Sequencing)
  ATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCCTGACGCAAAA
  GACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCG
  TCCCCGCAGGCCGCCTTCACCCAGCAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAA
  AGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGAAATGATCATAACCAAGGCTGGA
  AGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAAAACGAAGTAC
  ATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGCAGATAATAAA
  TGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGAC
  TCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTC
  ACCAACAACCACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAAATACCAG
  CCTAGATTACACATCGTGAAAGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCG
  TTCTGCACTCACGTCTTTCCTGAGACTGCGTTTATAGCAGTGACTTCTTACCAGAACCAC
  AAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCAAAGGATTTCGGGGCAGTGAT
  GACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGTGGTCCCCAGG
  AGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTCTCGAGCT
  CTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCC
  TCCCAGGACCTCCTGCCTCCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATT
  TACCATTGTACCAAGAGGAAAGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAG
  CCCTACATGGAGACATCACCCAGTGAAGAAGATTCCTTCTACCGCTCTAGCTATCCACAG
  CAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCACAGCGGCAAGCTTGCATGTAT
  GCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAGCTGCAACACG
  TGGCCAAGCATGCCTTCCTACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGG
  CTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCT
  GGCATGGCCAACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCC
  GTGGCCCACCAGCCTGTGGTCAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTCCCCTGGC
  ACCCTTCAGCCCCCTGAGTTCCTCTACTCTCATGGCGTGCCAAGGACTCTATCCCCTCAT
  CAGTACCACTCTGTGCACGGAGTTGGCATGGTGCCAGAGTGGAGCGACAATAGCTAA
  
  Clone variation with respect to NM_000192.3
  648 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000192 unedited
  CCGACACTCGTCATTTTGTNAATACGNACTTTCTNTNAGGGNNCGGCCGCGNAATTTCGG
  CACCAGGATAAACAGTAATATTTAATTTGTCGGAGACCACAAACCAACCTTGAGCTGGGA
  GGTACGTGCTCTTCTTGACAGACGTTGGAAGAAGACCTGGCCTAAAGAGGTCTCTTTTGG
  TGGTCCTTTTCAAAGTCTTCACCTGAGCCCTGCTCTCCAGCGAGGCGCACTCCTGGCTTT
  TGCGCTCCAAAGAAGAGGTGGGATAGTTGGAGAGCAGAACCTTGCGCGGGCACAGGGCCC
  TGGGCGCACCATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCC
  TGACGCAAAAGACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAA
  GTCCCCGTCGTCCCCGCAGGCCGCCTTCACCCAGCAGGGCATGGAGGGAATCAAAGTGTT
  TCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGAAATGATCATAAC
  CAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAA
  AACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGC
  AGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGT
  GCACCCAGACTCCCCCGCCACCGGGGCGCATTGGATGAAGCAGCTCGTCTCCTTCCCAGA
  AACTCAGCTCACCACAACCACCTGGACCCTTTGGGCATATTATTCTAAATTCATGCANCA
  ATACCAGCCTAGATTACACATCGTGAAAGCGGATGAAAATATGGATTNGNCTCAAAAATA
  CACCGTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000192 unedited
  GGAAAAAAACAATATATTAACAAAAAAACACAAACTTGGTTTCTGAGTATCAATAAAATT
  AAAAGCTCTTGGCCAGCTCCTATGCTGGGTTTCATTTCATGTTATTACTTAATTAGAATT
  CCTATTTATAAATAAATAGTACCAGTAAAATATTACATCTGAAGAACCCTACCATAACAC
  TTTACTTACACACTTTTTCCAATACTGTTTTCGGCTTTCAGTAAACACAGTTCTGTGTCG
  GCATTGGTGTGGGCGTGGTTTCTTTGGCGAGATATCATTGCCGACTGTGCCCTGTCTCTT
  CTGTAGGAACCGTGTATTTTGCTTGACATCCACTTTGCCCCGTTTTTCACGGACGTTTGG
  AACGCTGGTTCCTGCCCTGTTTCCTCCTAACACATGCCTCTTTTATTAATAACCCCATTT
  CGGTTTTTCCGTCTCAACTCTTCCCACACCCTTCTCGCCCCTGCCATTACATTCCTGCCG
  CACCGTCCACCGCGTTTCTTTCCTCTCGCCCATTTCCGCCTTTCCCCCCTTTTTTTTCTT
  TTCTCCCCCCCCCCCGACAGGCATTTTTTCAGTCTTTCCCGCGCTTTCCGGCGTCGCCCT
  TTTTTTCCCCCCTTCTCCTTTTTCCCGTATCGGGCCCCCTCCTTTTCCCGCCGTTTTCCC
  CCCGCCATTTTTTGGTTTTTTTTTTTCGCGCCCCCGCGTTGTGCAAAACCCGCTCTCTCC
  CCCCCCTTTTTTCTCGTTGTTTTTTTCTCCCCCCTCTTTTTTCTCCTCTTGTTTTTTGTG
  TCCACGTTTTCCGAATTGCCCTCGTCACCCGCCGGCTCTGCCGGCCCCTTTTTTTTTTTT
  TCGCCCCCTTTTTTCCCCCTCCCCCCCGTCTTTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000192
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000192.3, NP_000183.2
• RefSeq Size: 3921 bp
• RefSeq ORF: 1557 bp
• Locus ID: 6910
• Cytogenetics: 12q24.21
• Domains: T-box
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.