EXT1 (NM_000127) Human Untagged Clone

CAT#: SC120098

EXT1 (untagged)-Human exostosin 1 (EXT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXT1
• Synonyms: EXT; LGCR; LGS; TRPS2; TTV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120098 sequence for NM_000127 edited (data generated by NextGen Sequencing)
  ATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTG
  TTTTATTTCGGAGGCTTGCAGTTTAGGGCATCGAGGAGCCACAGCCGGAGAGAAGAACAC
  AGCGGTAGGAATGGCTTGCACCACCCCAGTCCGGATCATTTCTGGCCCCGCTTCCCGGAC
  GCTCTGCGCCCCTTCGTTCCTTGGGATCAATTGGAAAACGAGGATTCCAGCGTGCACATT
  TCCCCCCGGCAGAAGCGAGATGCCAACTCCAGCATCTACAAAGGCAAGAAGTGCCGCATG
  GAGTCCTGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTACGTATACCCA
  CAGCAAAAAGGGGAGAAAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGC
  TCCAGGTTCTACACCTCGGACCCCAGCCAGGCGTGCCTCTTTGTCCTGAGTCTGGATACT
  TTAGACAGAGACCAGTTGTCACCTCAGTATGTGCACAATTTGAGATCCAAAGTGCAGAGT
  CTCCACTTGTGGAACAATGGTAGGAATCATTTAATTTTTAATTTATATTCCGGCACTTGG
  CCTGACTACACCGAGGACGTGGGGTTTGACATCGGCCAGGCGATGCTGGCCAAAGCCAGC
  ATCAGTACTGAAAACTTCCGACCCAACTTTGATGTTTCTATTCCCCTCTTTTCTAAGGAT
  CATCCCAGGACAGGAGGGGAGAGGGGGTTTTTGAAGTTCAACACCATCCCTCCTCTCAGG
  AAGTACATGCTGGTATTCAAGGGGAAGAGGTACCTGACAGGGATAGGATCAGACACCAGG
  AATGCCTTATATCACGTCCATAACGGGGAGGACGTTGTGCTCCTCACCACCTGCAAGCAT
  GGCAAAGACTGGCAAAAGCACAAGGATTCTCGCTGTGACAGAGACAACACCGAGTATGAG
  AAGTATGATTATCGGGAAATGCTGCACAATGCCACTTTCTGTCTGGTTCCTCGTGGTCGC
  AGGCTTGGGTCCTTCAGATTCCTGGAGGCTTTGCAGGCTGCCTGTGTCCCTGTGATGCTC
  AGCAATGGATGGGAGTTGCCATTCTCTGAAGTGATTAATTGGAACCAAGCTGCCGTCATA
  GGCGATGAGAGATTGTTATTACAGATTCCTTCTACAATCAGGTCTATTCATCAGGATAAA
  ATCCTAGCACTTAGACAGCAGACACAATTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAG
  AAGATTGTATTAACTACACTAGAGATTATTCAGGACAGAATATTCAAGCACATATCACGT
  AACAGTTTAATATGGAACAAACATCCTGGAGGATTGTTCGTACTACCACAGTATTCATCT
  TATCTGGGAGATTTTCCTTACTACTATGCTAATTTAGGTTTAAAGCCCCCCTCCAAATTC
  ACTGCAGTCATCCATGCGGTGACCCCCCTGGTCTCTCAGTCCCAGCCAGTGTTGAAGCTT
  CTCGTGGCTGCAGCCAAGTCCCAGTACTGTGCCCAGATCATAGTTCTATGGAATTGTGAC
  AAGCCCCTACCAGCCAAACACCGCTGGCCTGCCACTGCTGTGCCTGTCGTCGTCATTGAA
  GGAGAGAGCAAGGTTATGAGCAGCCGTTTTCTGCCCTACGACAACATCATCACAGACGCC
  GTGCTCAGCCTTGACGAGGACACGGTGCTTTCAACAACAGAGGTGGATTTCGCCTTCACA
  GTGTGGCAGAGCTTCCCTGAGAGGATTGTGGGGTACCCCGCGCGCAGCCACTTCTGGGAT
  AACTCTAAGGAGCGGTGGGGATACACATCAAAGTGGACGAACGACTACTCCATGGTGTTG
  ACAGGAGCTGCTATTTACCACAAATATTATCACTACCTATACTCCCATTACCTGCCAGCC
  AGCCTGAAGAACATGGTGGACCAATTGGCCAATTGTGAGGACATTCTCATGAACTTCCTG
  GTGTCTGCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAGAAGCAGTATAAGGAG
  ACAATGATGGGACAGACTTCTCGGGCTTCCCGTTGGGCTGACCCTGACCACTTTGCCCAG
  CGACAGAGCTGCATGAATACGTTTGCCAGCTGGTTTGGCTACATGCCGCTGATCCACTCT
  CAGATGAGGCTCGACCCCGTCCTCTTTAAAGACCAGGTCTCTATTTTGAGGAAGAAATAC
  CGAGACATTGAGCGACTTTGA
  
  Clone variation with respect to NM_000127.2
  1065 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000127 unedited
  GGGTCANNATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCACTAGCC
  GGCTGAACCCGGGATCGGCTGACTTGTTGGAACCGGAGTGCTCTGCACGGAGAGTGGTGG
  ATGAGTTGAAGTTGCCTTCCCGGGGCTCATTTTCCACGCTGCCGAGAGGAATCCGAGAGG
  CAAGGCAATCACTTCGTCTTGCCATTGATTGGGTATCGGGAGCTTTTTTTTTCTCCTCTC
  TCTCTTTCTTTTCCTCCGTCTTGTTGCATGCAAGAAAATTACAGTCCGCTGCTCGCCCGC
  CCTGGGTGCGAGATATTCAGCCCCGCTCTCTCCCGTGCATTGTGCAACCCAAAGATGAAA
  GACCGAAGGGGAGAAAGTTAAAGAAATCGCCCACATGCGCTGGATCAGTCCACGGCTTGG
  GGAAAGGCATCCAGAGAAGGTGGGAGCGGAGAGTTTGAAGTCTTTACAGGCGGGAAGATG
  GCGGACTGGAGCTGAAAGTGTTGATTGGGAAACTTGGGTGATTCTTGTGTTTATTTACAA
  TCCTCTTGACCCAGGCAGGACACATGCAGGCCAAAAAACGCTATTTCATCCTGCTCTCAG
  CTGGCTCTTGTCTCGCCCTTTTGTTTTATTTCGGAGGCTTGCAGTTTAAGGCATCGAGGA
  GCCACAGCCGGAGAGAAGAACACAGCGGTAGGAATGGCTTGCACCACCCCAGTCCGGATC
  ATTTCTGGCCCCGCTTCCCGGACGCTCTGCGCCCCTTCGTTCCTTGGGATCAATTGGAAA
  ACGAGGATTCCAGCGTGCACATTTCCCCCCGGCAGAAGCGAGATGCCAACTCCAGCATCT
  ACAAAGGGCAGAAGTGCCGCATGGAGTCCTGCTTCGATTTCACCTTTTGCAGAAANNACG
  CTCAAGTCTACGTATACCCACAGCAAAGGGGAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000127 unedited
  NNGGTCNTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAA
  CCCCCCCAATGAAAAACGATTATCATATATATCTATCTCAAACATAGAGATTTCAGTTAG
  AACTGCCTACCTGGAAGTCGTAATACTTTCTTTCATGCAGCGACATTTTAGAGATAAAAG
  TTCAAGTGGATTTGAAGATTCGAAGTGGAAAACAATTTGCACTGGCAAAGTTGAATAATG
  AAGTTACAACTTATGCTCAGTTATTTCTTGATAATGGGTAGAGTGACTTATTGTGTTTTC
  CACGAAGTTTGAGCTTTTGAAACTGCAGGGCACCCTACATGGCCATGACAATGATGTCTG
  TGGGAAAAGGAATAGCAGCTTTGAAATATTCACAACCAACACAATTTTGATTAAACAAAG
  AACTCTGGTTTTTAATAGTTTTGATCATTAAAAAAGTTTAAACCTGCATAGCAATCATTT
  CAAAAATAATTATTTAATGTTCCATAATTAAACTGTACACAACCTAGTCTTGGGACACAG
  AAGCCAGTGAGGTGAGTTTGGAGCAGTCTGGTGCAGTCCCAGGAGCCAGGAGTTGAGTTC
  TCATTGGCCTTTTTTTTTTTGTCATTCTGCTCATCTAAGTTTTTGGATAGTTGGCACAAT
  CTGGCTCTGCTGATGAGTGGATCTGCACTGGGAAGAGAGAGCAGCTTGACCCCCATCCCT
  TCTTGCTTTCCCTCCCCACTCAGCCGGATCCTCAAGTCGCTCAATGTCTCGGTATTTCTT
  CCTCAAATANAACCTGGTCTTAAAGAGGACCGGGGCGAGCCTTATCTGAAATGGATCAGC
  GGCTGTACCCAACCAGCTGGCAACGTTTTATGCAACTTGGCCCTGGGCAAGGGCCAGGGT
  CANCCCACGGGAACCCCGAAAGCTGTCCATCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000127
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000127.2, NP_000118.2
• RefSeq Size: 3376 bp
• RefSeq ORF: 2241 bp
• Locus ID: 2131
• Cytogenetics: 8q24.11
• Domains: Exostosin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq, Jul 2008]'