EXT1 (NM_000127) Human Untagged Clone

CAT#: SC320682

EXT1 (untagged)-Human exostosin 1 (EXT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXT1
• Synonyms: EXT; LGCR; LGS; TRPS2; TTV
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000127.2 CGCTGGATCAGTCCACGGCTTGGGGAAAGGCATCCAGAGAAGGTGGGAGCGGAGAGTTTG
  AAGTCTTTACAGGCGGGAAGATGGCGGACTGGAGCTGAAAGTGTTGATTGGGAAACTTGG
  GTGATTCTTGTGTTTATTTACAATCCTCTTGACCCAGGCAGGACACATGCAGGCCAAAAA
  ACGCTATTTCATCCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTGTTTTATTTCGGAGG
  CTTGCAGTTTAGGGCATCGAGGAGCCACAGCCGGAGAGAAGAACACAGCGGTAGGAATGG
  CTTGCACCACCCCAGTCCGGATCATTTCTGGCCCCGCTTCCCGGACGCTCTGCGCCCCTT
  CGTTCCTTGGGATCAATTGGAAAACGAGGATTCCAGCGTGCACATTTCCCCCCGGCAGAA
  GCGAGATGCCAACTCCAGCATCTACAAAGGCAAGAAGTGCCGCATGGAGTCCTGCTTCGA
  TTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTACGTATACCCACAGCAAAAAGGGGA
  GAAAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGCTCCAGGTTCTACAC
  CTCGGACCCCAGCCAGGCGTGCCTCTTTGTCCTGAGTCTGGATACTTTAGACAGAGACCA
  GTTGTCACCTCAGTATGTGCACAATTTGAGATCCAAAGTGCAGAGTCTCCACTTGTGGAA
  CAATGGTAGGAATCATTTAATTTTTAATTTATATTCCGGCACTTGGCCTGACTACACCGA
  GGACGTGGGGTTTGACATCGGCCAGGCGATGCTGGCCAAAGCCAGCATCAGTACTGAAAA
  CTTCCGACCCAACTTTGATGTTTCTATTCCCCTCTTTTCTAAGGATCATCCCAGGACAGG
  AGGGGAGAGGGGGTTTTTGAAGTTCAACACCATCCCTCCTCTCAGGAAGTACATGCTGGT
  ATTCAAGGGGAAGAGGTACCTGACAGGGATAGGATCAGACACCAGGAATGCCTTATATCA
  CGTCCATAACGGGGAGGACGTTGTGCTCCTCACCACCTGCAAGCATGGCAAAGACTGGCA
  AAAGCACAAGGATTCTCGCTGTGACAGAGACAACACCGAGTATGAGAAGTATGATTATCG
  GGAAATGCTGCACAATGCCACTTTCTGTCTGGTTCCTCGTGGTCGCAGGCTTGGGTCCTT
  CAGATTCCTGGAGGCTTTGCAGGCTGCCTGCGTCCCTGTGATGCTCAGCAATGGATGGGA
  GTTGCCATTCTCTGAAGTGATTAATTGGAACCAAGCTGCCGTCATAGGCGATGAGAGATT
  GTTATTACAGATTCCTTCTACAATCAGGTCTATTCATCAGGATAAAATCCTAGCACTTAG
  ACAGCAGACACAATTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAGAAGATTGTATTAAC
  TACACTAGAGATTATTCAGGACAGAATATTCAAGCACATATCACGTAACAGTTTAATATG
  GAACAAACATCCTGGAGGATTGTTCGTACTACCACAGTATTCATCTTATCTGGGAGATTT
  TCCTTACTACTATGCTAATTTAGGTTTAAAGCCCCCCTCCAAATTCACTGCAGTCATCCA
  TGCGGTGACCCCCCTGGTCTCTCAGTCCCAGCCAGTGTTGAAGCTTCTCGTGGCTGCAGC
  CAAGTCCCAGTACTGTGCCCAGATCATAGTTCTATGGAATTGTGACAAGCCCCTACCAGC
  CAAACACCGCTGGCCTGCCACTGCTGTGCCTGTCGTCGTCATTGAAGGAGAGAGCAAGGT
  TATGAGCAGCCGTTTTCTGCCCTACGACAACATCATCACAGACGCCGTGCTCAGCCTTGA
  CGAGGACACGGTGCTTTCAACAACAGAGGTGGATTTCGCCTTCACAGTGTGGCAGAGCTT
  CCCTGAGAGGATTGTGGGGTACCCCGCGCGCAGCCACTTCTGGGATAACTCTAAGGAGCG
  GTGGGGATACACATCAAAGTGGACGAACGACTACTCCATGGTGTTGACAGGAGCTGCTAT
  TTACCACAAATATTATCACTACCTATACTCCCATTACCTGCCAGCCAGCCTGAAGAACAT
  GGTGGACCAATTGGCCAATTGTGAGGACATTCTCATGAACTTCCTGGTGTCTGCTGTGAC
  AAAATTGCCTCCAATCAAAGTGACCCAGAAGAAGCAGTATAAGGAGACAATGATGGGACA
  GACTTCTCGGGCTTCCCGTTGGGCTGACCCTGACCACTTTGCCCAGCGACAGAGCTGCAT
  GAATACGTTTGCCAGCTGGTTTGGCTACATGCCGCTGATCCACTCTCAGATGAGGCTCGA
  CCCCGTCCTCTTTAAAGACCAGGTCTCTATTTTGAGGAAGAAATACCGAGACATTGAGCG
  ACTTTGAGGAATCCGGCTGAGTGGGGGAGGGGAAGCAAGAAGGGATGGGGGTCAAGCTGC
  TCTCTCTTCCCAGTGCAGATCCACTCATCAGCAGAGCCAGATTGTGCCAACTATCCAAAA
  ACTTAGATGAGCAGAATGACAAAAAAAAAAAGGCCAATGAGAACTCAACTCCTGGCTCCT
  GGGACTGCACCAGACTGCTCCAAACTCACCTCACTGGCTTCTGTGTCCCAAGACTAGGTT
  GTGTACAGTTTAATTATGGAACATTAAATAATTATTTTTGAAATGATTGCTATGCAGGTT
  TAAACTTTTTTAATGATCAAAACTATTAAAAACCAGAGTTCTTTGTTTAATCAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000127
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000127.2, NP_000118.2
• RefSeq Size: 3376 bp
• RefSeq ORF: 2241 bp
• Locus ID: 2131
• Cytogenetics: 8q24.11
• Domains: Exostosin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: 'This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses. [provided by RefSeq, Jul 2008]'