Myosin (MYL6) (NM_079423) Human Untagged Clone

CAT#: SC120266

MYL6 (untagged)-Human myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL6
• Synonyms: ESMLC; LC17; LC17-GI; LC17-NM; LC17A; LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120266 sequence for NM_079423 edited (data generated by NextGen Sequencing)
  ATGTGTGACTTCACCGAAGACCAGACCGCAGAGTTCAAGGAGGCCTTCCAGCTGTTTGAC
  CGAACAGGTGATGGCAAGATCCTGTACAGCCAGTGTGGGGATGTGATGAGGGCCCTGGGC
  CAGAACCCTACCAACGCCGAGGTGCTCAAGGTCCTGGGGAACCCCAAGAGTGATGAGATG
  AATGTGAAGGTGCTGGACTTTGAGCACTTTCTGCCCATGCTGCAGACAGTGGCCAAGAAC
  AAGGACCAGGGCACCTATGAGGATTATGTCGAAGGACTTCGGGTGTTTGACAAGGAAGGA
  AATGGCACCGTCATGGGTGCTGAAATCCGGCATGTTCTTGTCACACTGGGTGAGAAGATG
  ACAGAGGAAGAAGTAGAGATGCTGGTGGCAGGGCATGAGGACAGCAATGGTTGTATCAAC
  TATGAAGAGCTCGTCCGCATGGTGCTGAATGGCTGA
  
  Clone variation with respect to NM_079423.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_079423 unedited
  ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCAGGAAAGGTCCCGGAGAGCTG
  AGCAGTCAAGATGTGTGACTTCACCGAAGACCAGACCGCAGAGTTCAAGGAGGCCTTCCA
  GCTGTTTGACCGAACAGGTGATGGCAAGATCCTGTACAGCCAGTGTGGGGATGTGATGAG
  GGCCCTGGGCCAGAACCCTACCAACGCCGAGGTGCTCAAGGTCCTGGGGAACCCCAAGAG
  TGATGAGATGAATGTGAAGGTGCTGGACTTTGAGCACTTTCTGCCCATGCTGCAGACAGT
  GGCCAAGAACAAGGACCAGGGCACCTATGAGGATTATGTCGAAGGACTTCGGGTGTTTGA
  CAAGGAAGGAAATGGCACCGTCATGGGTGCTGAAATCCGGCATGTTCTTGTCACACTGGG
  TGAGAAGATGACAGAGGAAAGAGTAGAGATGCTGGTGGCAGGGCATGAGGACAGCAATGG
  TTGTATCAACTATGAAGAGCTCGTCCGCATGGTGCTGAATGGCTGANGACCTTCCCAGTC
  TCCCAGAGTCCGTGCCTTTCCCTGTGTGAATTTTGTATCTAGCCTAAATTTTCCTTAGCC
  TTCTTGTCTCAGAACTTTCCATCTTGCTTTCTTGGATGATGTTGCGGCAGCATTACCAAA
  TAAACTTGTTTCTGGGAAAAAAAANANAAANNNAAAAANNANAANAAAANNAANAAAAAA
  AANANAAAAACAAAAAANNNANAANANAAAANACAAAAAAAAAAAANAANNNAAAAAACC
  TCCTTCTTTTTGGGGCGGGTATATTTTTCTAAAAACCCGGGTGATTTTGGCCCCCCCAGT
  GTTCTCGGCTGGAGGCCCTCCGGCCCCCCCTTGATAAAAAAAAGGATATTTTGGAAGGCG
  CTATGANGGGGGNGGGGGGGGGGNNNNNNNNNNNCNNNCNNNNNNACNCNCCACCACCGC
  CCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_079423 unedited
  CCTGGGCAGTTTTTTGGTGAAGCTGACGGCAACTCATCCAANANAGACAAGATGGGAAAG
  TTGCTGAGACAAGAAAGCCTAGGGAAACTTTAGGCTAGATACAAAATTCACACAGGGAAA
  GGCACGGACTCTGGGGAGACTGGGAAGGTCCTCAGCCATTCAGCACCATGCGGACGAGCT
  CTTCATAGTTGATACAACCATTGCTGTCCTCATGCCCTGCCACCAGCATCTCTACTTCTT
  CCTCTGTCATCTTCTCACCCAGTGTGACAAGAACATGCCGGATTTCAGCACCCATGACGG
  TGCCATTTCCTTCCTTGTCAAACACCCGAAGTCCTTCGACATAATCCTCATAGGTGCCCT
  GGTCCTTGTTCTTGGCCACTGTCTGCAGCATGGGCAGAAAGTGCTCAAAGTCCAGCACCT
  TCACATTCATCTCATCACTCTTGGGGTTCCCCAGGACCTTGAGCACCTCGGCGTTGGTAG
  GGTTCTGGCCCAGGGCCCTCATCACATCCCCACACTGGCTGTACAGGATCTTGCCATCAC
  CTGTTCGGTCAAACAGCTGGAAGGCCTCCTTGAACTCTGCGGTCTGGTCTTCGGTGAAGT
  CACACATCTTGACTGCTCAGCTCTCCGGGACCTTTTCCTGCAGCCTCGTGCCGAATTCGC
  GGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTT
  ATATAGACCTNCCACCGTACACGCCTACCGGCCATTTGCGTCAACGGGGCCGGGTTATTA
  CGACATTTTGGGAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATG
  GGGTGGGAGACCTGGGAATTCCCGTGAGTCAAACCGCTTCCACGCCCATTGGTGTACTGC
  CAAACCCCATTACCATGGTATAACGATGACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_079423
• Insert Size: 4880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_079423.1, NP_524147.1
• RefSeq Size: 651 bp
• RefSeq ORF: 351 bp
• Locus ID: 4637
• Cytogenetics: 12q13.2
• Domains: EFh
• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (2) is the same length as isoform 1, but the proteins differ in their C-terminal sequences.