WHIP (WRNIP1) (NM_130395) Human Untagged Clone

CAT#: SC120325

WRNIP1 (untagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WRNIP1
• Synonyms: bA420G6.2; WHIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_130395, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGTGAGCGGGCCGGAAGACGACCCCTTCCTTTCGCAGCTGCACCAGGTGCAGTGCCCCGTGTGCC
  AGCAGATGATGCCCGCCGCGCACATCAACTCGCACCTGGACCGCTGTCTGCTGCTCCACCCGGCGGGGCA
  CGCGGAGCCCGCGGCCGGGTCGCACCGCGCCGGGGAGCGGGCCAAGGGGCCCTCGCCGCCCGGCGCCAAG
  AGGCGGCGGCTGTCGGAGAGCTCGGCGCTGAAGCAGCCAGCCACCCCGACGGCAGCCGAGAGCAGCGAGG
  GCGAGGGTGAGGAGGGCGACGACGGCGGCGAGACCGAGAGCCGCGAGAGCTACGACGCGCCGCCCACACC
  CAGCGGCGCCCGCCTTATCCCCGACTTCCCGGTGGCCCGCTCCAGCAGCCCCGGGAGGAAGGGGTCGGGG
  AAGAGGCCGGCGGCCGCCGCCGCGGCGGGGAGCGCGTCTCCGCGCAGCTGGGACGAGGCGGAGGCGCAGG
  AGGAGGAGGAGGCCGTGGGCGACGGCGATGGCGACGGGGACGCGGACGCGGACGGCGAGGACGACCCGGG
  GCACTGGGACGCGGACGCTGCCGAAGCCGCCACCGCCTTCGGGGCCAGTGGCGGGGGCCGCCCGCACCCC
  CGGGCGCTGGCTGCCGAGGAGATCCGACAGATGCTACAGGGCAAGCCGCTGGCCGACACGATGCGTCCTG
  ACACGCTGCAGGATTACTTCGGGCAGAGCAAGGCCGTGGGCCAGGATACCCTGCTGCGCTCGCTCCTGGA
  GACCAACGAAATCCCCTCGCTTATCCTGTGGGGGCCGCCGGGCTGCGGCAAGACCACTCTGGCTCACATC
  ATAGCCAGCAACAGCAAGAAACATAGCATAAGGTTTGTGACATTATCTGCAACAAATGCCAAGACAAATG
  ATGTGCGAGATGTCATAAAACAAGCTCAAAATGAAAAGAGCTTTTTCAAAAGGAAAACCATCCTTTTTAT
  TGATGAGATTCATCGGTTCAATAAATCTCAGCAGGTCAACGCTGCTCTTCTGAGCCGCTGTCGAGTGATT
  GTTCTTGAGAAGCTTCCAGTAGAGGCAATGGTGACTATTTTAATGCGAGCGATCAACTCCCTGGGAATCC
  ACGTCCTAGACTCTAGCCGTCCCACTGACCCTCTGAGCCACAGCAGCAACAGCAGCTCAGAGCCCGCCAT
  GTTCATAGAGGATAAAGCAGTAGACACCCTGGCTTACCTCAGTGACGGTGACGCCCGAGCTGGGTTGAAC
  GGACTGCAGCTGGCGGTGCTGGCTAGGTTAAGCTCTAGGAAGATGTTCTGTAAGAAGAGTGGGCAATCCT
  ATTCTCCCAGTAGAGTTCTGATCACAGAGAATGACGTGAAGGAGGGCCTACAGCGATCCCACATTTTATA
  TGACCGGGCAGGTGAGGAGCATTACAACTGCATCTCCGCCCTGCACAAGTCCATGCGGGGCTCAGACCAG
  AACGCCTCCCTCTACTGGCTGGCTCGCATGCTCGAGGGAGGAGAGGACCCACTCTACGTGGCACGGAGGC
  TTGTCAGGTTTGCCAGCGAGGACATAGGTCTGGCAGACCCGTCTGCGTTAACACAAGCGGTTGCTGCCTA
  CCAAGGCTGTCATTTTATAGGCATGCCTGAATGTGAGGTGCTTCTGGCCCAGTGTGTGGTCTACTTTGCC
  AGAGCCCCAAAGTCCATTGAGGTGTACAGCGCCTACAACAACGTCAAAGCCTGCCTGAGGAACCACCAGG
  GGCCACTGCCCCCCGTGCCCCTGCACCTGAGGAACGCGCCCACTAGGCTGATGAAGGATTTGGGCTATGG
  CAAAGGCTACAAGTACAACCCCATGTACAGCGAGCCTGTGGATCAGGAGTACCTGCCTGAAGAGTTGAGG
  GGGGTAGATTTCTTCAAGCAGAGGAGGTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130395 unedited
  GTTCACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGACGACCCGG
  GGCACTGGGACGCGGACGCTGCCGAAGCCGCCACCGCCTTCGGGGCCAGCGGCGGGGGCC
  GCCCGCACCCCCGGGCGCTGGCTGCCGAGGAGATCCGACAGATGCTACAGGGCAAGCCGC
  TGGCCGACACGATGCGTCCTGACACGCTGCAGGATTACTTCGGGCAGAGCAAGGCCGTGG
  GCCAGGATACCCTGCTGCGCTCGCTCCTGGAGACCAACGAAATCCCCTCGCTTATCCTGT
  GGGGGCCGCCGGGCTGCGGCAAGACCACTCTGGCTCACATCATAGCCAGCAACAGCAAGA
  AACATAGCATAAGGTTTGTGACATTATCTGCAACAAATGCCAAGACAAATGATGTGCGAG
  ATGTCATAAAACAAGCTCAAAATGAAAAGAGCTTTTTCAAAAGGAAAACCATCCTTTTTA
  TTGATGAGATTCATCGGTTCAATAAATCTCAGCAGGACACTTTCCTTCCTCACGTGGAAT
  GTGGGACGATCACTCTGATTGGGGCAACCACTGAAAACCCTTCCTTCCAGGTCAACGCTG
  CTCTTCTGAGCCGCTGTCGAGTGATTGTTCTTGAGAAGCTTCCAGTAGAGGCAATGGTGA
  CTATNNTAATGCGAGCGATCAACTCCCTGGGAATCCACGTCCTAGACTCTAGCCGTCCCA
  CTGACCCTCTGAGCCACAGCAGCAACAGCAGCTCAGAGCCCGCCATGGTCATAGAGGATA
  AAGCAGTAGACACCCTGGCTTACCTCAGTGACGGTGACGCCCGAGCTGGGTTGAACGGAC
  TGCAGCTGGGCGTGCTGGCTANGTTAAGCTCTNAGAAAGATGTTCTGTAGAAGAGTGGGG
  CATCCTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130395 unedited
  TTTGGCCGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTACTTTGAAATAGT
  TTTATTGGCTCGTAAGACCTTTGCATATAAAAAAATACCCAAAACACTATGCAGTATTAA
  ATCACAATTACATTTTTTTACCAATTAAAACTACCCAGAATATACATTTTTTAAAGAAAA
  GAAAATCCTACAGAACTGAATTCTGAAATGACATTATGACTTAAATACTATGACAAAATA
  GATAATTCCTTATAACATTAATTCATTGCACAAAGCTGCCAGGTATTTTCATAAGCATAA
  CCGCTGCACCGAGTGCAAATGAACACAATTTCAAAGTTACACATTTATTCGAAAGAACTG
  CGCCTCCACCTATGGAACTCTTAAATTTCTGGCACAAAATGTTGGTCTGTTCTAACTTCC
  ACCAGGCAACCAACTTTGCAATCCACTAACTTTCTTTCTGGCCCTCCCTTAAAAAAGCAA
  CATCCTTCTGCTGTCGTGCCCTGAGGAGTCAGCACCTCCTCTGCTTGAAGAAATCTACCC
  CCCTCAACTCTTCAGGCAGGTACTCCTGATCCACAGGCTCGCTGTACATGGGGTTGTACT
  TGTAGCCTTTGCCATAGCCCAAATCCTTCATCAGCCTAGTGGGCGCGTTCCTCAGGTGCA
  GGGGCACGGGGGGCAGTGGCCCCTGGTGGTTNCTCAGGCAGGCTTTGACGTTGTTGTAGG
  CGCTGGACACTCAATGGGACTTGGGGCTCTGGCAAGTANACCACCACTGGGCCGNAAGCA
  CTNACATTCAGCATGCTATAAATGACGCCTGGNTAGCAGCACCGCTTGTGTAACGCANAA
  GGTCTGGCAGACTTATGTCTGCTTGCAAACTGACAGNCTNCGGGCAAGTAAAGTGTTTCT
  TTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130395
• ORF Size: 1923 bp
• Insert Size: 2250
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_130395.1, NP_569079.1
• RefSeq Size: 2592
• RefSeq ORF: 1923
• Locus ID: 56897
• Domains: AAA, AAA, ZnF_Rad18
• Gene Summary: Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (2) utilizes a different acceptor splice site for an internal coding exon, compared to transcript variant 1. This variant maintains the same reading frame but encodes an isoform (2) which is 25 aa shorter than isoform 1.