WHIP (WRNIP1) (NM_130395) Human Untagged Clone

CAT#: SC320312

WRNIP1 (untagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WRNIP1
• Synonyms: bA420G6.2; WHIP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_130395.1 CATGAGCGGCGCCCTCCTCCGGCCCGCGAGCGTCCTGCTGGTTCCCCGAGCGAGGGTCTC
  GCGGCGCGGGGCCTAGCGGAGGGCATCGAAGGCCTCCGCGTGCGCACGGGTTGCTGCGGC
  CGCGCCGGGCGCCGGGGAGGGCGGCGGCCGCCATGGAGGTGAGCGGGCCGGAAGACGACC
  CCTTCCTTTCGCAGCTGCACCAGGTGCAGTGCCCCGTGTGCCAGCAGATGATGCCCGCCG
  CGCACATCAACTCGCACCTGGACCGCTGTCTGCTGCTCCACCCGGCGGGGCACGCGGAGC
  CCGCGGCCGGGTCGCACCGCGCCGGGGAGCGGGCCAAGGGGCCCTCGCCGCCCGGCGCCA
  AGAGGCGGCGGCTGTCGGAGAGCTCGGCGCTGAAGCAGCCAGCCACCCCGACGGCAGCCG
  AGAGCAGTGAGGGCGAGGGCGAGGAGGGCGACGACGGCGGCGAGACCGAGAGCCGCGAGA
  GCTACGACGCGCCGCCCACACCCAGCGGCGCCCGCCTTATCCCCGACTTCCCGGTGGCCC
  GCTCCAGCAGCCCCGGGAGGAAGGGGTCGGGGAAGAGGCCGGCGGCCGCCGCCGCGGCGG
  GGAGCGCGTCTCCGCGCAGCTGGGACGAGGCGGAGGCGCAGGAGGAGGAGGAGGCCGTGG
  GCGACGGCGATGGCGACGGGGACGCGGACGCGGACGGCGAGGACGACCCGGGGCACTGGG
  ACGCGGACGCTGCCGAAGCCGCCACCGCCTTCGGGGCCAGCGGCGGGGGCCGCCCGCACC
  CCCGGGCGCTGGCTGCCGAGGAGATCCGACAGATGCTACAGGGCAAGCCGCTGGCCGACA
  CGATGCGTCCTGACACGCTGCAGGATTACTTCGGGCAGAGCAAGGCCGTGGGCCAGGATA
  CCCTGCTGCGCTCGCTCCTGGAGACCAACGAAATCCCCTCGCTTATCCTGTGGGGGCCGC
  CGGGCTGCGGCAAGACCACTCTGGCTCACATCATAGCCAGCAACAGCAAGAAACATAGCA
  TAAGGTTTGTGACATTATCTGCAACAAATGCCAAGACAAATGATGTGCGAGATGTCATAA
  AACAAGCTCAAAATGAAAAGAGCTTTTTCAAAAGGAAAACCATCCTTTTTATTGATGAGA
  TTCATCGGTTCAATAAATCTCAGCAGGTCAACGCTGCTCTTCTGAGCCGCTGTCGAGTGA
  TTGTTCTTGAGAAGCTTCCAGTAGAGGCAATGGTGACTATTTTAATGCGAGCGATCAACT
  CCCTGGGAATCCACGTCCTAGACTCTAGCCGTCCCACTGACCCTCTGAGCCACAGCAGCA
  ACAGCAGCTCAGAGCCCGCCATGTTCATAGAGGATAAAGCAGTAGACACCCTGGCTTACC
  TCAGTGACGGTGACGCCCGAGCTGGGTTGAACGGACTGCAGCTGGCGGTGCTGGCTAGGT
  TAAGCTCTAGGAAGATGTTCTGTAAGAAGAGTGGGCAATCCTATTCTCCCAGTAGAGTTC
  TGATCACAGAGAATGACGTGAAGGAGGGCCTACAGCGATCCCACATTTTATATGACCGGG
  CAGGTGAGGAGCATTACAACTGCATCTCCGCCCTGCACAAGTCCATGCGGGGCTCAGACC
  AGAACGCCTCCCTCTACTGGCTGGCTCGCATGCTCGAGGGAGGAGAGGACCCACTCTACG
  TGGCACGGAGGCTTGTCAGGTTTGCCAGCGAGGACATAGGTCTGGCAGACCCGTCTGCGT
  TAACACAAGCGGTTGCTGCCTACCAAGGCTGTCATTTTATAGGCATGCCTGAATGTGAGG
  TGCTTCTGGCCCAGTGTGTGGTCTACTTTGCCAGAGCCCCAAAGTCCATTGAGGTGTACA
  GCGCCTACAACAACGTCAAAGCCTGCCTGAGGAACCACCAGGGGCCACTGCCCCCCGTGC
  CCCTGCACCTGAGGAACGCGCCCACTAGGCTGATGAAGGATTTGGGCTATGGCAAAGGCT
  ACAAGTACAACCCCATGTACAGCGAGCCTGTGGATCAGGAGTACCTGCCTGAAGAGTTGA
  GGGGGGTAGATTTCTTCAAGCAGAGGAGGTGCTGACTCCTCAGGGCACGACAGCAGAAGG
  ATGTTGCTTTTTTAAGGGAGGGCCAGAAAGAAAGTTAGTGGATTGCAAAGTTGGTTGCCT
  GGTGGAAGTTAGAACAGACCAACATTTTGTGCCAGAAATTTAAGAGTTCCATAGGTGGAG
  GCGCAGTTCTTTCGAATAAATGTGTAACTTTGAAATTGTGTTCATTTGCACTCGGTGCAG
  CGGTTATGCTTATGAAAATACCTGGCAGCTTTGTGCAATGAATTAATGTTATAAGGAATT
  ATCTATTTTGTCATAGTATTTAAGTCATAATGTCATTTCAGAATTCAGTTCTGTAGGATT
  TTCTTTTCTTTAAAAAATGTATATTCTGGGTAGTTTTAATTGGTAAAAAAATGTAATTGT
  GATTTAATACTGCATAGTGTTTTGGGTATTTTTTTTATATGCAAAGGTCTTACGAGCCAA
  TAAAACTATTTCAAAGTAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130395.1 unedited
  GTGCGTTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCG
  AGGAGATCTGCCGCCGCGATCGCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGACC
  GACGCGTTAAGCGGCCGCGAATTCAGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTAA
  AACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGCA
  CGAGGCATGAGCGGCGCCCTCCTCCGGCCCGCGAGCGTCCTGCTGGTTCCCCGAGCGAGG
  GTCTCGCGGCGCGGGGCCTAGCGGAGGGCATCGAAGGCCTCCGCGTGCGCACGGGTTGCT
  GCGGCCGCGCCGGGCGCCGGGGAGGGCGGCGGCCGCCATGGAGGTGAGCGGGCCGGAAGA
  CGACCCCTTCCTTTCGCAGCTGCACCAGGTGCAGTGCCCCGTGTGCCAGCAGATGATGCC
  CGCCGCGCACATCAACTCGCACCTGGACCGCTGTCTGCTGCTCCACCCGGCGGGGCACGC
  GGAGCCCGCGGCCGGGTCGCACCGCGCCGGGGAGCGGGCCAAGGGGCCCTCGCCGCCCGG
  CGCCAAGAGGCGGCGGCTGTCGGAGAGCTCGGCGCTGAAGCAGCCAGCCACCCCGACGGC
  AGCCGAGAGCAGTGAGGGCGAGGGCGAGGAGGGCGACGACGGCGGCGAGACCGAGAGCCG
  CGAGAGCTACGACGCGCCGCCCACACCCAGCGGCGCCCGCCTTATCCCCGACTTCCCGGT
  GGCCCGCTCCAGCAGCCCCGGAGGAAGGGTCGGGGAAGAGCCGGCGCCGCCGCCGCGCGG
  GGAGCGCGTCTCGCGCAGCTGGGACAGCGAGGCGCAGAGAGAGAGGCCGTGGCGACGCGA
  TGCACGGGACCCGACCGACGGCAGGACAACCCGGGGCACTGGTACCGACGCCTGCCGAAG
  CCGCACCCGCCCTTCCAG
• Restriction Sites: Please inquire
• ACCN: NM_130395
• ORF Size: 1923 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_130395.1, NP_569079.1
• RefSeq Size: 2592
• RefSeq ORF: 1923
• Locus ID: 56897
• Domains: AAA, AAA, ZnF_Rad18
• Gene Summary: Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (2) utilizes a different acceptor splice site for an internal coding exon, compared to transcript variant 1. This variant maintains the same reading frame but encodes an isoform (2) which is 25 aa shorter than isoform 1.