PLP1 (NM_199478) Human Untagged Clone

CAT#: SC121107

PLP1 (untagged)-Human proteolipid protein 1 (PLP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLP1
• Synonyms: GPM6C; HLD1; MMPL; PLP; PLP/DM20; PMD; SPG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_199478, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCTTGTTAGAGTGCTGTGCAAGATGTCTGGTAGGGGCCCCCTTTGCTTCCCTGGTGGCCACTGGAT
  TGTGTTTCTTTGGGGTGGCACTGTTCTGTGGCTGTGGACATGAAGCCCTCACTGGCACAGAAAAGCTAAT
  TGAGACCTATTTCTCCAAAAACTACCAAGACTATGAGTATCTCATCAATGTGATCCATGCCTTCCAGTAT
  GTCATCTATGGAACTGCCTCTTTCTTCTTCCTTTATGGGGCCCTCCTGCTGGCTGAGGGCTTCTACACCA
  CCGGCGCAGTCAGGCAGATCTTTGGCGACTACAAGACCACCATCTGCGGCAAGGGCCTGAGCGCAACGTT
  TGTGGGCATCACCTATGCCCTGACCGTTGTGTGGCTCCTGGTGTTTGCCTGCTCTGCTGTGCCTGTGTAC
  ATTTACTTCAACACCTGGACCACCTGCCAGTCTATTGCCTTCCCCAGCAAGACCTCTGCCAGTATAGGCA
  GTCTCTGTGCTGATGCCAGAATGTATGGTGTTCTCCCATGGAATGCTTTCCCTGGCAAGGTTTGTGGCTC
  CAACCTTCTGTCCATCTGCAAAACAGCTGAGTTCCAAATGACCTTCCACCTGTTTATTGCTGCATTTGTG
  GGGGCTGCAGCTACACTGGTTTCCCTGCTCACCTTCATGATTGCTGCCACTTACAACTTTGCCGTCCTTA
  AACTCATGGGCCGAGGCACCAAGTTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199478 unedited
  CGCGAATTCGGCACGAGGCCTTCCAGCTGAACAAAGTCAGCCACAAAGCAGACTAGCCAG
  CCGGCTACAATTGGAGTCAGAGTCCCAAAGACATGGGCTTGTTAGAGTGCTGTGCAAGAT
  GTCTGGTAGGGGCCCCCTTTGCTTCCCTGGTGGCCACTGGATTGTGTTTCTTTGGGGTGG
  CACTGTTCTGTGGCTGTGGACATGAAGCCCTCACTGGCACAGAAAAGCTAATTGAGACCT
  ATTTCTCCAAAAACTACCAAGACTATGAGTATCTCATCAATGTGATCCATGCCTTCCAGT
  ATGTCATCTATGGAACTGCCTCTTTCTTCTTCCTTTATGGGGCCCTCCTGCTGGCTGAGG
  GCTTCTACACCACCGGCGCAGTCAGGCAGATCTTTGGCGACTACAAGACCACCATCTGCG
  GCAAGGGCCTGAGCGCAACGTTTGTGGGCATCACCTATGCCCTGACCGTTGTGTGGCTCC
  TGGTGTTTGCCTGCTCTGCTGTGCCTGTGTACATTTACTTCAACACCTGGACCACCTGCC
  AGTCTATTGCCTTCCCCAGCAAGACCTCTGCCAGTATAGGCAGTCTCTGTGCTGATGCCA
  GAATGTATGGTGTTCTCCCATGGAATGCTTTCCCTGGCAAGGGTTGTGGCTCCAACCTTC
  TGTCCATCTGCAAAACAGCTGAGTTCCAAATGACCNTNCACCTGTTTATTGCTGCATTTG
  NGGGGGCTGCAGCTACACTGGTTTCCCTGCTCACCTTCATGANTGCTGNCACTTACAACT
  TTGNCGTCCTTAACTCATGGGCCGAGGACCANTTTTGATCCCCCGTAAAAATCCCCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199478
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199478.1, NP_955772.1
• RefSeq Size: 2933 bp
• RefSeq ORF: 729 bp
• Locus ID: 5354
• Cytogenetics: Xq22.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a transmembrane proteolipid protein that is the predominant component of myelin. The encoded protein may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively splicing results in multiple transcript variants, including the DM20 splice variant. [provided by RefSeq, Feb 2015]'
  Transcript Variant: This variant (2, also known as DM20) uses an alternate in-frame donor splice site compared to variant 1. It encodes a shorter isoform (2) than isoform 1.