Calcipressin 1 (RCAN1) (NM_203418) Human Untagged Clone

CAT#: SC121454

RCAN1 (untagged)-Human regulator of calcineurin 1 (RCAN1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RCAN1
• Synonyms: ADAPT78; CSP1; DSC1; DSCR1; MCIP1; RCN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121454 sequence for NM_203418 edited (data generated by NextGen Sequencing)
  ATGCATTTTAGAAACTTTAACTACAGTTTTAGCTCCCTGATTGCCTGTGTGGCAAACAGT
  GATATCTTCAGCGAAAGTGAAACCAGGGCCAAATTTGAGTCCCTCTTTAGGACGTATGAC
  AAGGACATCACCTTTCAGTATTTTAAGAGCTTCAAACGAGTCAGAATAAACTTCAGCAAC
  CCCTTCTCCGCAGCAGATGCCAGGCTCCAGCTGCATAAGACTGAGTTTCTGGGAAAGGAA
  ATGAAGTTATATTTTGCTCAGACCTTACACATAGGAAGCTCACACCTGGCTCCGCCAAAT
  CCAGACAAGCAGTTTCTGATCTCCCCTCCCGCCTCTCCGCCAGTGGGATGGAAACAAGTG
  GAAGATGCGACCCCAGTCATAAACTATGATCTCTTATATGCCATCTCCAAGCTGGGGCCA
  GGGGAAAAGTATGAATTGCACGCAGCGACTGACACCACTCCCAGCGTGGTGGTCCATGTA
  TGTGAGAGTGATCAAGAGAAGGAGGAAGAAGAGGAAATGGAAAGAATGAGGAGACCTAAG
  CCAAAAATTATCCAGACCAGGAGGCCGGAGTACACGCCGATCCACCTCAGCTGA
  
  Clone variation with respect to NM_203418.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_203418 unedited
  NGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGAATTCGCCGAGCTCACTAGGGCT
  TGACTGCGTGGGTCTGTAGCGCTTTCACTGTAAGAAAGCAAGATGCATTTTAGAAACTTT
  AACTACAGTTTTAGCTCCCTGATTGCCTGTGTGGCAAACAGTGATATCTTCAGCGAAAGT
  GAAACCAGGGCCAAATTTGAGTCCCTCTTTAGGACGTATGACAAGGACATCACCTTTCAG
  TATTTTAAGAGCTTCAAACGAGTCAGAATAAACTTCAGCAACCCCTTCTCCGCAGCAGAT
  GCCAGGCTCCAGCTGCATAAGACTGAGTTTCTGGGAAAGGAAATGAAGTTATATTTTGCT
  CAGACCTTACACATAGGAAGCTCACACCTGGCTCCGCCAAATCCAGACAAGCAGTTTCTG
  ATCTCCCCTCCCGCCTCTCCGCCAGTGGGATGGAAACAAGTGGAAGATGCGACCCCAGTC
  ATAAACTATGATCTCTTATATGCCATCTCCAAGCTGGGGCCAGGGGAAAAGTATGAATTG
  CACGCAGCGACTGACACCACTCCCAGCGTGGTGGTCCATGTATGTGAGAGTGATCAAGAG
  AAGGAGGAAGAAGAGGAAATGGAAAGAATGAGGAGACCTAAGCCAAAAATTATCCAGACC
  AGGAGGCCGGAGTACACGCCGATCCACCTCAGCTGAACTGGCACGCGACGAGGACGCATT
  CCAAATCATACTCACGGGAGGAATCTTTTACTGTGGNAGGTGGCTGGTCACGACTTCTNT
  CGGAGTGGCAGCCGAGATCGGNGTGGCAGAAATCCCAGTTCATGTTGCTCAGAGAGAATA
  AGGCCGTGTCCCCTGTTCTATGCTGACACCGTTACTGTTCATGCACCCGGAATGACTTGG
  CCATCACTGATTGNGGTGATCGACAAGACATTTGGACTGTCTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_203418
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203418.1, NP_981963.1
• RefSeq Size: 2434 bp
• RefSeq ORF: 594 bp
• Locus ID: 1827
• Cytogenetics: 21q22.12
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]'
  Transcript Variant: This variant (3) uses an alternate 5' exon compared to variant 1. The resulting isoform (c) has a distinct N-terminus compared to isoform a.