SPHK1 (NM_182965) Human Untagged Clone

CAT#: SC121602

SPHK1 (untagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHK1
• Synonyms: SPHK
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182965, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGCTCAAGTTCTGGGATTTTTACGCAGCTGGACTCCCCTCCCCCTGGCAGCCCCGAGGGGTCCAG
  CCGCCGCAGGGAATGACGCCGGTGCTCCTGCAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCG
  GCCCTGCGACGCCCGCCTGGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCC
  CCCACAGCGCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCCCC
  GGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAAGGCCTT
  GCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACT
  GAGCGGCGGAACCACGCGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCA
  TGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCAT
  CCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCT
  GGCTATGAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGT
  CACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGCCTCTTCTCTGTGCTCAGCCTGGCCTG
  GGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTG
  GGCACCTTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAG
  TGGGTTCCAAGACACCTGCCTCCCCCGTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACT
  GGAGGAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCACTGCTG
  CACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGT
  TCTACGTGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCA
  TATGGAGTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCCTTCCGCTTGGAGCCCAAGGATGGG
  AAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACT
  ACTTCTGGATGGTCAGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGA
  AGAGCCCTTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182965 unedited
  TCTCTACCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGCGACTGGGTAGGGCCGCCCACCCTGCCTTCGCGCCGCTCGTGGC
  TCCTGTGCGGCCCGCCCTCGCGGGGCCCCGGGAACTGGGCCACTTGTCGCTTGGGCGAGA
  GCAGGCGGCAGCTGGTGGCCCGGTTCGGGCTTGGCTTTGGCGCGACCCGGGAGCGGCTCC
  CACGAGCGCCGCGCGCGTCGCAACGGAGCGGGGCCCTGAGAAGCGCGCGCCGCGGCTCCC
  ACCGCTCTGGAGCTCCGGGCAGGGGACACGGCAACCTGGATGGCTGGGGCAGGGATCCTC
  TCCCAGAACTTCGTGCCCAGCAATGTCCGCTCAAGTTCTGGGATTTTTACGCAGCTGGAC
  TCCCCTCCCCCTGGCAGCCCCGAGGGGTCCAGCCGCCGCAGGGAATGACGCCGGTGCTCC
  TACAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCCTGCGACGCCCGCCT
  GGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGC
  GCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCC
  CCGGAGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCAGCGGCAA
  GGGCAAGGGCCTGCAGCTCTTCCAGAGTCACGTGCAGCCCCTTTTGGCTTGAAGCTGAAT
  CTCCTTACGCTGATGCTCACTGAGCGGCGGACCACGCGCGGGAGCTGGTGCGGTCCGAGA
  ACTGGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182965 unedited
  TTGCACCATTGGGCGATGTGCAACTTCCCAGGNCCAGNAAAGCACTGGGGAAGGGTCACA
  GGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAAGTCA
  CTTTATTTGGATTTGGTTCCGGGGGGGGGGGTCTCAAAACAAACTAAAAGGCCTTACATA
  GGCAGCTGGGCCCAACCCGCTGGGCTCCTGACCCAAGACTTTATTCTGGCCCGTCCCCCC
  AAAACATAACCCCCACCTTCTCACCCTTTTCCAAAAGAATCTCCCCCCCCCCCACAAGAA
  CTGTGGACAGGCCCTGCAACCCTAAGGAAAGAAGAAAGGTCCCGCAAGTAAACACTAAAG
  CACAGCGCCGCCCAAGGGTCATAAAGGCTCTTCTGGCGGTGGCATCTGCTGGGGCTTCCA
  GCTGGGCGGGGGCTCCACGCCACCCCTGACCATCCAAAAATATTTTGGGTGCACCTGGCC
  CTGCACGGCCTCCCTAACCATCAATTCCCCATCCACTGGAAACACACCTTTCCCATCCTT
  GGGCTCCAAGCGGAAGGCGACCACGGGCACCTATACCAAGTAGGGGCATTCATACTCCAT
  ATGCCCGCCCTTCTCCATGGCCAGGAAGAAGCGCAACAACATGGCACGAGACACTCCCGC
  CCCCACGTAAAACAAATGCCTGACCCCCGCTGCACAACGGCCCATGGGTGCAGCAAACAT
  CTCACTGCCCACGTGCCAATGCCGCAATGCCACGACTATCACAAAATCCTCGTCGGGCCC
  ACTGTCCAGTGAGAGGGCCTGGCTCCTCCCATGGCCAAGGTGTTGCATCTACCGGGCCCT
  GCTTGGACCCAACGGGGA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_182965
• ORF Size: 1413 bp
• Insert Size: 2200
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_182965.1, NP_892010.1
• RefSeq Size: 2174
• RefSeq ORF: 1413
• Locus ID: 8877
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).