SPHK1 (NM_182965) Human Untagged Clone

CAT#: SC323952

SPHK1 (untagged)-Human sphingosine kinase 1 (SPHK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHK1
• Synonyms: SPHK
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_182965.1 GAAAAGTTTGAGGCCGGAGGGAGCGAGGCCGGGGAGTCCGCTCCAGCGGGGCGCTCCAGT
  CCCTCAGACGTGGGCTGAGCTTGGGACGAGCTGCGTTCCGCCCCAGGCCACTGTAGGGAA
  CGGCGGTGGCGCCTCCCCAGCAAACCGGACCGACTGGGTAGGGCCGCCCACCCTGCCTTC
  GCGCCGCTCGTGGCTCCTGTGCGGCCCGCCCTCGCGGGGCCCCGGGAACTGGGCCACTTG
  TCGCTTGGGCGAGAGCAGGCGGCAGCTGGTGGCCCGGTTCGGGCTTGGCTTTGGCGCGAC
  CCGGGAGCGGCTCCCACGAGCGCCGCGCGCGTCGCAACGGAGCGGGGCCCTGAGAAGCGC
  GCGCCGCGGCTCCCACCGCTCTGGAGCTCCGGGCAGGGGACACGGCAACCTGGATGGCTG
  GGGCAGGGATCCTCTCCCAGAACTTCGTGCCCAGCAATGTCCGCTCAAGTTCTGGGATTT
  TTACGCAGCTGGACTCCCCTCCCCCTGGCAGCCCCGAGGGGTCCAGCCGCCGCAGGGAAT
  GACGCCGGTGCTCCTACAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCC
  TGCGACGCCCGCCTGGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGC
  AGCGCCCCCACAGCGCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGAT
  CCAGCGGGCGGCCCCCGGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAAC
  CCGCGCGGCGGCAAGGGCAAGGCCTTGCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTG
  GCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACTGAGCGGCGGAACCACGCGCGGGAG
  CTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTGGAGACGGG
  CTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTGACTGGGAGACCGCCATCCAG
  AAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCAT
  TATGCTGGCTATGAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTG
  TGCCGCCGGCTGCTGTCACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGC
  CTCTTCTCTGTGCTCAGCCTGGCCTGGGGCTTCATTGCTGATGTGGACCTAGAGAGTGAG
  AAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTGGGCACCTTCCTGCGTCTGGCAGCC
  CTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTTCCAAGACA
  CCTGCCTCCCCCGTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAG
  GAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCA
  CTGCTGCACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCT
  GGCGTCATGCATCTGTTCTACGTGCGGGCGGGAGTGTCTCGTGCCATGCTGCTGCGCCTC
  TTCCTGGCCATGGAGAAGGGCAGGCATATGGAGTATGAATGCCCCTACTTGGTATATGTG
  CCCGTGGTCGCCTTCCGCTTGGAGCCCAAGGATGGGAAAGGTGTGTTTGCAGTGGATGGG
  GAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCTGGATGGTC
  AGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAG
  CCCTTATGACCCCTGGGCCGCGCTGTGCCTTAGTGTCTACTTGCAGGACCCTTCCTCCTT
  CCCTAGGGCTGCAGGGCCTGTCCACAGCTCCTGTGGGGGTGGAGGAGACTCCTCTGGAGA
  AGGGTGAGAAGGTGGAGGCTATGCTTTGGGGGGACAGGCCAGAATGAAGTCCTGGGTCAG
  GAGCCCAGCTGGCTGGGCCCAGCTGCCTATGTAAGGCCTTCTAGTTTGTTCTGAGACCCC
  CACCCCACGAACCAAATCCAAATAAAGTGACATTCCCAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182965.1 unedited
  GGCCATTTGTATACGACTCCTATAGGGCGGCCGGCGTATCGCGGCGCGCCGGTACCGGTC
  CGGAATTCCCGGGATGAAAAGTTTGAGGCCGGAGGGAGCGAGGCCGGGGAGTCCGCTCCA
  GCGGGGCGCTCCAGTCCCTCAGACGTGGGCTGAGCTTGGGACGAGCTGCGTTCCGCCCCA
  GGCCACTGTAGGGAACGGCGGTGGCGCCTCCCCAGCAAACCGGACCGACTGGGTAGGGCC
  GCCCACCCTGCCTTCGCGCCGCTCGTGGCTCCTGTGCGGCCCGCCCTCGCGGGGCCCCGG
  GAACTGGGCCACTTGTCGCTTGGGCGAGAGCAGGCGGCAGCTGGTGGCCCGGTTCGGGCT
  TGGCTTTGGCCCGACCCGGGAGCGGCCCCCACGAGCGCCGCGCGCCTTCCATCGTATCCC
  GGCACTGAGAAGCGAGTGCTGCAGATAACCCTCGCTCTTGAATCTTCCGGCACTTGAC
• Restriction Sites: ECoRI-NOT
• ACCN: NM_182965
• ORF Size: 1413 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_182965.1, NP_892010.1
• RefSeq Size: 2174
• RefSeq ORF: 1413
• Locus ID: 8877
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).