CENPA (NM_001809) Human Untagged Clone

CAT#: SC121879

CENPA (untagged)-Human centromere protein A (CENPA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CENPA
• Synonyms: CenH3; CENP-A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121879 sequence for NM_001809 edited (data generated by NextGen Sequencing)
  ATGGGCCCGCGCCGCCGGAGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCG
  ACCCCGACCCCCGGCCCCTCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACAC
  AGTCGGCGGAGACAAGGTTGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTC
  TTGATAAGGAAGCTGCCCTTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGT
  GGTGTGGACTTCAATTGGCAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCA
  TTTCTAGTTCATCTCTTTGAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACT
  CTCTTCCCAAAGGATGTGCAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGC
  TGA
  
  Clone variation with respect to NM_001809.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001809 unedited
  NAACGTTTTGATTTGTAAACGACTTATATAGGCGGCCGACGAATTCGCACGAGGGCAGCC
  CGAGCAGGAGCCGTGGGACCGGGCGCCGGCACCCTCTGCGGCGTGTCATGGGCCCGCGCC
  GCCGGAGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCGACCCCGACCCCCG
  GCCCCTCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACACAGTCGGCGGAGAC
  AAGGTTGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTCTTGATAAGGAAGC
  TGCCCTTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGTGGTGTGGACTTCA
  ATTGGCAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCATTTCTAGTTCATC
  TCTTTGAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTCCCAAAGG
  ATGTGCAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCTCCTGCAC
  CCAGTGTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGACTCTCCAG
  AGCCATGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTCTGAACAG
  CTGGAGTGCAGCAGCACGATCTCGGCTCACTGGCACCTCCAACTCCCAAGTCAAGCGATT
  CTCCTGCCTCAGCCTCTCGGACCCTGTCTCTATTTTTTTTTTAATAAAGAAATAAAATGC
  TGTGTAACTTCATAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGC
  TGTTTCCCTGACAGATCCCCGGTGGCATCCCTGTGACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001809 unedited
  GGCCCATTGGCGATGGTCACTTGCCAGGTCCAGNATAGCACTGGGGNAGGGTCACAGGGC
  TGCCACCCGGGTATCTGTTGAGGAAAACAGCTATGACCGCGGCCGCAATCTATAGTCGAG
  TTTTTTTTTTTTTTTTTATGAAGTTACACAGAATTTTTATTTCTTTATTTAAAAAAAAAT
  AGAGACAGGGTCCGAGAGGCTGAGGCAGGAGAATCGCTTGAACTTGGGAGTTGGAGGTTG
  CAGTGAGCCGATATCGTGCTGCTGCACTCCAGCTGTTCAGAGACAGCAAAGTCCAGACAG
  CATCGCAGAATCCATTGGATCTAGTCATGGCTCTGGAGAGTCCCCGGTATCATCCCCCCT
  GGCTGAGCAGGAAAGACTGACAGAAACACTGGGTGCAGGAGCTCAGCCGAGTCCCTCCTC
  AAGGCCCCGGATCCTCCGGGCCAGTTGCACATCCTTTGGGAAGAGAGTAACTCGGCCTGC
  ATGTAAGGTGAGGAGATAGGCGTCCTCAAAGAGATGAACTAGAAATGCTTCTGCTGCCTC
  TTGTAGGGCCAATAGGGCCTGGGCTTGCCAATTGAAGTCCACACCACGAGTGAATTTAAC
  ACATATTTCTCTTGCCAGGCGGCTGAAGGGCAGCTTCCTTATCAAGAGGTGTGTGCTCTT
  CTGAAGCTTTCGGATCTCCTTTAGCCAACCTTGTCTCCGCCGACTGTGTTGATGGGAGGA
  AGCGCCTAAAGAGGGGCCCCGCCGGGAGGGGCCGGGGGTCGGGGTCGGGCTCGGGCTGCG
  CCTCCTCGGGGCCTCGGACTTTCGGCTNCGGGGGCGCGGGCCATGACACGCCGCATAGGG
  TGCCGGGCGCCGGTCCAACGCTTCTGCTCGGGCTGCCCCTCGTGCCGAATTCGCGCCGCC
  TATAATGAGTCGTATTACAAATGCTGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001809
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001809.2, NP_001800.1
• RefSeq Size: 1389 bp
• RefSeq ORF: 423 bp
• Locus ID: 1058
• Cytogenetics: 2p23.3
• Gene Summary: 'Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).