KCNN4 (NM_002250) Human Untagged Clone

CAT#: SC121952

KCNN4 (untagged)-Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNN4
• Synonyms: DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; KCa3.1; KCA4; SK4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121952 sequence for NM_002250 edited (data generated by NextGen Sequencing)
  ATGGGCGGGGATCTGGTGCTTGGCCTGGGGGCCTTGAGACGCCGAAAGCGCTTGCTGGAG
  CAGGAGAAGTCTCTGGCCGGCTGGGCACTGGTGCTGGCAGGAACTGGCATTGGACTCATG
  GTGCTGCATGCAGAGATGCTGTGGTTCGGGGGGTGCTCGTGGGCGCTCTACCTGTTCCTG
  GTTAAATGCACGATCAGCATTTCCACCTTCTTACTCCTCTGCCTCATCGTGGCCTTTCAT
  GCCAAAGAGGTCCAGCTGTTCATGACCGACAACGGGCTGCGGGACTGGCGCGTGGCGCTG
  ACCGGGCGGCAGGCGGCGCAGATCGTGCTGGAGCTGGTGGTGTGTGGGCTGCACCCGGCG
  CCCGTGCGGGGCCCGCCGTGCGTGCAGGATTTAGGGGCGCCGCTGACCTCCCCGCAGCCC
  TGGCCGGGATTCCTGGGCCAAGGGGAAGCGCTGCTGTCCCTGGCCATGCTGCTGCGTCTC
  TACCTGGTGCCCCGCGCCGTGCTCCTGCGCAGCGGCGTCCTGCTCAACGCTTCCTACCGC
  AGCATCGGCGCTCTCAATCAAGTCCGCTTCCGCCACTGGTTCGTGGCCAAGCTTTACATG
  AACACGCACCCTGGCCGCCTGCTGCTCGGCCTCACGCTTGGCCTCTGGCTGACCACCGCC
  TGGGTGCTGTCCGTGGCCGAGAGGCAGGCTGTTAATGCCACTGGGCACCTTTCAGACACA
  CTTTGGCTGATCCCCATCACATTCCTGACCATCGGCTATGGTGACGTGGTGCCGGGCACC
  ATGTGGGGCAAGATCGTCTGCCTGTGCACTGGAGTCATGGGTGTCTGCTGCACAGCCCTG
  CTGGTGGCCGTGGTGGCCCGGAAGCTGGAGTTTAACAAGGCAGAGAAGCACGTGCACAAC
  TTCATGATGGATATCCAGTATACCAAAGAGATGAAGGAGTCCGCTGCCCGAGTGCTACAA
  GAAGCCTGGATGTTCTACAAACATACTCGCAGGAAGGAGTCTCATGCTGCCCGCAGGCAT
  CAGCGCAAGCTGCTGGCCGCCATCAACGCGTTCCGCCAGGTGCGGCTGAAACACCGGAAG
  CTCCGGGAACAAGTGAACTCCATGGTGGACATCTCCAAGATGCACATGATCCTGTATGAC
  CTGCAGCAGAATCTGAGCAGCTCACACCGGGCCCTGGAGAAACAGATTGACACGCTGGCG
  GGGAAGCTGGATGCCCTGACTGAGCTGCTTAGCACTGCCCTGGGGCCGAGGCAGCTTCCA
  GAACCCAGCCAGCAGTCCAAGTAG
  
  Clone variation with respect to NM_002250.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002250 unedited
  GGCAAAGCGCNAAAAANACNNNACTCTCCCCCCNCNNCGGTTCAGAATTTGTNATACGAC
  TCACTATAGGGCGGCCGCGAATTCGCACCAGGGCAGGAAGCTGGCTGAGCCCCAAAACCC
  CGGGGGCCATGGGCGGGGATCTGGTGCTTGGCCTGGGGGCCTTGAGACGCCGAAAGCGCT
  TGCTGGAGCAGGAGAAGTCTCTGGCCGGCTGGGCACTGGTGCTGGCAGGAACTGGCATTG
  GACTCATGGTGCTGCATGCAGAGATGCTGTGGTTCGGGGGGTGCTCGTGGGCGCTCTACC
  TGTTCCTGGTTAAATGCACGATCAGCATTTCCACCTTCTTACTCCTCTGCCTCATCGTGG
  CCTTTCATGCCAAAGAGGTCCAGCTGTTCATGACCGACAACGGGCTGCGGGACTGGCGCG
  TGGCGCTGACCGGGCGGCAGGCGGCGCAAATCGTGCTGGAGCTGGTGGTGTGTGGGCTGC
  ACCCGGCGCCCGTGCGGGGCCCGCCGTGCGTGCAGGATTTAGGGGCGCCGCTGACCTCCC
  CGCAGCCCTGGCCGGGATTCCTGGGCCAAGGGGAAGCGCTGCTGTCCCTGGCCATGCTGC
  TGCGTCTCTACCTGGTGCCCCGCGCCGTGCTCCTGCGCAGCGGCGTCCTGCTCAACGCTT
  NCTACCGCAGCATCGGCGCTCTCAATCAAGTCCGCTTCCGCCACTGGTTCGTGGCCCAGC
  TTTACATGAACACGCNACCCTGGCCGNCTGCTGNCTCGCCTCACGCTTGGCCTCTGGCTG
  ACCACCGNCTGNGTGCTGTCCGNTGGCGANAAGCTGTTAATGCCACTGGGCACCTTTAGA
  CCACTTTGGCTGACCCCATACATTCCTGACATCGNTATGGGGAACTGTGCCGGCCACATG
  TGGGGCCAGATCTCTGGCTGGCACTGGAGCATGGTGTCTGCTGACAGCCCTGCTGTGGCC
  GGGTGGCCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002250 unedited
  NCCGTTCTACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTGGCTTTAACATTTATTACAAAGAAAAGAGCAGAGGCTGGTGAGTTACACTTCTTC
  CTCCCCACCAGGTGCTCTCTGCAGCTCTGGAAAAATGGTGTCCTCTTTGTTGTCCCACCA
  GGGGGCGCCACCTCCAGCCCCGCCCCAGCCTCATACCCAGTTCTTCAGCTCGGCCAGCGG
  TAACTGAAGCCTCCCAGAATCCTGGATCCGGGCCCCTAGTACCCTCTTTCCCAGGGACCC
  AGGAGTCCTGCCTCCAGTCGCCTGCACTTGTAACTGAGAGCTGGAGGTCGTCCATAGCAG
  CATAGTGAGAGTGTTTTTGATGAGGGTATGCAGAGTGGGGGTGACCATGTTCCCACCTGG
  GGCCTCAGGTGGGCCAAGGCCTACCCACTTTAGCCAGCGTCCCCTCCAGCAGCCATCAGC
  AAGCCAACCCACTCCAAGCCAGGGCCCCCTTTGGTCCTTGCACTTGAGGTGCTTTGTTCA
  GGGCTGGGTCAGGAGTGGCAGAGACGATGTCCACCACCTCAGTACTGGGGAAAGTAGCCT
  GGTTCCTCCTCGTGGGTCCAGCTACTTGGACTGCTGGCTGGGTTCTGGAAGCTGCCTCGG
  CCCCAGGGCAGTGCTAAGCAGCTCAGTCAGGGCATCCAGCTTCCCCGCCAGCGTGTCAAT
  CTGTTTCTCCAGGGCCCGGTGTGAGCTGCTCANATTCTGCTGCAGGTCATACAGGATCAT
  GTGCATCTTGGAGATGTCCACCATGGAGTTCACTTGTTCCCGGAGCTCTCGTGTTTCAAC
  CGCACCCTGCGGACCCGTTGTGGCGGCCAGCAACTTGCGCTGATGCCTGCCGGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002250
• Insert Size: 2050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002250.2, NP_002241.1
• RefSeq Size: 2240 bp
• RefSeq ORF: 1284 bp
• Locus ID: 3783
• Cytogenetics: 19q13.31
• Domains: SK_channel, CaMBD
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]'