KCNN4 (NM_002250) Human Untagged Clone

CAT#: SC322489

KCNN4 (untagged)-Human potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (KCNN4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNN4
• Synonyms: DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCA1; KCa3.1; KCA4; SK4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002250 edited
  CCGGGGGCCATGGGCGGGGATCTGGTGCTTGGCCTGGGGGCCTTGAGACGCCGAAAGCGC
  TTGCTGGAGCAGGAGAAGTCTCTGGCCGGCTGGGCACTGGTGCTGGCAGGAACTGGCATT
  GGACTCATGGTGCTGCATGCAGAGATGCTGTGGTTCGGGGGGTGCTCGTGGGCGCTCTAC
  CTGTTCCTGGTTAAATGCACGATCAGCATTTCCACCTTCTTACTCCTCTGCCTCATCGTG
  GCCTTTCATGCCAAAGAGGTCCAGCTGTTCATGACCGACAACGGGCTGCGGGACTGGCGC
  GTGGCGCTGACCGGGCGGCAGGCGGCGCAGATCGTGCTGGAGCTGGTGGTGTGTGGGCTG
  CACCCGGCGCCCGTGCGGGGCCCGCCGTGCGTGCAGGATTTAGGGGCGCCGCTGACCTCC
  CCGCAGCCCTGGCCGGGATTCCTGGGCCAAGGGGAAGCGCTGCTGTCCCTGGCCATGCTG
  CTGCGTCTCTACCTGGTGCCCCGCGCCGTGCTCCTGCGCAGCGGCGTCCTGCTCAACGCT
  TCCTACCGCAGCATCGGCGCTCTCAATCAAGTCCGCTTCCGCCACTGGTTCGTGGCCAAG
  CTTTACATGAACACGCACCCTGGCCGCCTGCTGCTCGGCCTCACGCTTGGCCTCTGGCTG
  ACCACCGCCTGGGTGCTGTCCGTGGCCGAGAGGCAGGCTGTTAATGCCACTGGGCACCTT
  TCAGACACACTTTGGCTGATCCCCATCACATTCCTGACCATCGGCTATGGTGACGTGGTG
  CCGGGCACCATGTGGGGCAAGATCGTCTGCCTGTGCACTGGAGTCATGGGTGTCTGCTGC
  ACAGCCCTGCTGGTGGCCGTGGTGGCCCGGAAGCTGGAGTTTAACAAGGCAGAGAAGCAC
  GTGCACAACTTCATGATGGATATCCAGTATACCAAAGAGATGAAGGAGTCCGCTGCCCGA
  GTGCTACAAGAAGCCTGGATGTTCTACAAACATACTCGCAGGAAGGAGTCTCATGCTGCC
  CGCAGGCATCAGCGCAAGCTGCTGGCCGCCATCAACGCGTTCCGCCAGGTGCGGCTGAAA
  CACCGGAAGCTCCGGGAACAAGTGAACTCCATGGTGGACATCTCCAAGATGCACATGATC
  CTGTATGACCTGCAGCAGAATCTGAGCAGCTCACACCGGGCCCTGGAGAAACAGATTGAC
  ACGCTGGCGGGGAAGCTGGATGCCCTGACTGAGCTGCTTAGCACTGCCCTGGGGCCGAGG
  CAGCTTCCAGAACCCAGCCAGCAGTCCAAGTAGCTGGACCCACGAGGAGGAACCAGGCTA
  CTTTCCCCAGTACTGAGGTGGTGGACATCGTCTCTGCCACTCCTGACCCAGCCCTGAACA
  AAGCACCTCAAGTGCAAGGACCAAAGGGGGCCCTGGCTTGGAGTGGGTTGGCTTGCTGAT
  GGCTGCTGGAGGGGACGCTGGCTAAAGTGGGTAGGCCTTGGCCCACCTGAGGCCCCAGGT
  GGGAACATGGTCACCCCCACTCTGCATACCCTCATCAAAAACACTCTCACTATGCTGCTA
  TGGACGACCTCCAGCTCTCAGTTACAAGTGCAGGCGACTGGAGGCAGGACTCCTGGGTCC
  CTGGGAAAGAGGGTACTAGGGGCCCGGATCCAGGATTCTGGGAGGCTTCAGTTACCGCTG
  GCCGAGCTGAAGAACTGGGTATGAGGCTGGGGCGGGGCTGGAGGTGGCGCCCCCTGGTGG
  GACAACAAAGAGGACACCATTTTTCCAGAGCTGCAGAGAGCACCTGGTGGGGAGGAAGAA
  GTGTAACTCACCAGCCTCTGCTCTTATCTTTGTAATAAATGTTAAAGCCAAAAAAAAAAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002250
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002250.2, NP_002241.1
• RefSeq Size: 2240 bp
• RefSeq ORF: 1284 bp
• Locus ID: 3783
• Cytogenetics: 19q13.31
• Domains: SK_channel, CaMBD
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]'