SFRP2 (NM_003013) Human Untagged Clone

CAT#: SC122029

SFRP2 (untagged)-Human secreted frizzled-related protein 2 (SFRP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFRP2
• Synonyms: FRP-2; SARP1; SDF-5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122029 sequence for NM_003013 edited (data generated by NextGen Sequencing)
  ATGCTGCAGGGCCCTGGCTCGCTGCTGCTGCTCTTCCTCGCCTCGCACTGCTGCCTGGGC
  TCGGCGCGCGGGCTCTTCCTCTTTGGCCAGCCCGACTTCTCCTACAAGCGCAGCAATTGC
  AAGCCCATCCCGGCCAACCTGCAGCTGTGCCACGGCATCGAATACCAGAACATGCGGCTG
  CCCAACCTGCTGGGCCACGAGACCATGAAGGAGGTGCTGGAGCAGGCCGGCGCTTGGATC
  CCGCTGGTCATGAAGCAGTGCCACCCGGACACCAAGAAGTTCCTGTGCTCGCTCTTCGCC
  CCCGTCTGCCTCGATGACCTAGACGAGACCATCCAGCCATGCCACTCGCTCTGCGTGCAG
  GTGAAGGACCGCTGCGCCCCGGTCATGTCCGCCTTCGGCTTCCCCTGGCCCGACATGCTT
  GAGTGCGACCGTTTCCCCCAGGACAACGACCTTTGCATCCCCCTCGCTAGCAGCGACCAC
  CTCCTGCCAGCCACCGAGGAAGCTCCAAAGGTATGTGAAGCCTGCAAAAATAAAAATGAT
  GATGACAACGACATAATGGAAACGCTTTGTAAAAATGATTTTGCACTGAAAATAAAAGTG
  AAGGAGATAACCTACATCAACCGAGATACCAAAATCATCCTGGAGACCAAGAGCAAGACC
  ATTTACAAGCTGAACGGTGTGTCCGAAAGGGACCTGAAGAAATCGGTGCTGTGGCTCAAA
  GACAGCTTGCAGTGCACCTGTGAGGAGATGAACGACATCAACGCGCCCTATCTGGTCATG
  GGACAGAAACAGGGTGGGGAGCTGGTGATCACCTCGGTGAAGCGGTGGCAGAAGGGGCAG
  AGAGAGTTCAAGCGCATCTCCCGCAGCATCCGCAAGCTGCAGTGCTAG
  
  Clone variation with respect to NM_003013.2
  132 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003013 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCCGCCGGCTGCCAGCT
  TTTCGGGGCCCCGAGTCGCACCCAGCGAAGAGAGCGGGCCCGGGACAAGCTCGAACTCCG
  GCCGCCTCGCCCTTCCCCGGCTCCGCTCCCTCTGCCCCCTCGGGGTCGCGCGCCCACGAT
  GCTGCAGGGCCCTGGCTCGCTGCTGCTGCTCTTCCTCGCCTCGCACTGCTGCCTGGGCTC
  GGCGCGCGGGCTCTTCCTCTTTGGCCAGCCCGACTTCTCCTACAAGCGCAGCAATTGCAA
  GCCCATCCCGGCCAACCTGCAGCTGTGCCACGGCATCGAATACCAGAACATGCGGCTGCC
  CAACCTGCTGGGCCACGAGACCATGAAGGAGGTGCTGGAGCAGGCCGGCGCTTGGATCCC
  GCTGGTCATGAAGCAGTGCCACCCGGACACCAAGAAGTTCCTGTGCTCGCTCTTCGCCCC
  CGTCTGCCTCGATGACCTAGACGAGACCATCCAGCCATGCCACTCGCTCTGCGTGCAGGT
  GAAGGACCGCTGCGCCCCGGTCATGTCCGCCTTCGGCTTCCCCTGGCCCGACATGCTTGA
  GTGCGACCGTTTCCCCCAGGACAACGACCTTTGCATCCCCCTCGCTAGCAGCGACCACCT
  CCTGCCAGCCACCGAGGAAGCTCCAAAGGTATGTGAAGCCTGCANAAATANAAATGATGA
  TGACCACGACATAATGGAAACGCTNTGTAAAATGATTCTGCACTGAAAATAAAAGTGAAN
  GGAGATACCTACCATCACCGAGATACCANAATCATCCTGGNAGACCCAGAGCAGACCATT
  TACAAGCCTGAACGGTGTGTCCGAAAGGGACCTGAGNAAATCGNTGCTGTGGCTCAAGAA
  CAGCTGCAGTGCACCCTGTGAGGAGATGAACGACATCACGCGCCTCATCTGTCATGGGAC
  GAAACAGGNNTGGGAGCTGTGATACCTCGTNGAACGGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003013
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003013.2, NP_003004.1
• RefSeq Size: 2005 bp
• RefSeq ORF: 888 bp
• Locus ID: 6423
• Cytogenetics: 4q31.3
• Protein Families: Secreted Protein
• Protein Pathways: Wnt signaling pathway
• Gene Summary: 'This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]'