CLCNKB (BC020873) Human Untagged Clone

CAT#: SC122220

CLCNKB (untagged)-Human cDNA clone MGC:24087 IMAGE:4608625, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCNKB
• Synonyms: Chloride channel, kidney, B; chloride channel Kb; ClC-K2; CLCKB; hClC-Kb; MGC24087; OTTHUMP00000011120
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC020873 edited
  GGGGGGAGGATGTTGATTGTTGGAACACACACCTGTCCAGGTTCAGGGGAGCTGGAGGCT
  CTGTGAGAGGAGGGCCAGCTCAGCCACAGCAGGAGGACTGACAGGGGCCTGATGGAGGAG
  TTTGTGGGGCTGCGTGAAGGCTCCTCAGGGAACCCTGTGACTCTGCAGGAGCTGTGGGGC
  CCCTGTCCCCGCATCCGCCGAGGCATCCGAGGTGGCCTGGAGTGGCTGAAGCAGAAGCTC
  TTCCGCCTGGGCGAGGACTGGTACTTCCTGATGACCCTCGGGGTGCTCATGGCCCTGGTC
  AGCTGTGCCATGGACTTGGCTGTTGAGAGTGTGGTCCGAGCGCACCAGTGGCTGTACAGG
  GAGATTGGGGACAGCCACCTGCTCCGGTATCTCTCCTGGACTGTGTACCCTGTGGCCCTC
  GTCTCTTTCTCTTCGGGCTTCTCTCAGAGCATCACACCCTCCTCTGGAGGTTCTGGAATC
  CCGGAGGTGAAGACCATGTTGGCGGGTGTGGTCTTGGAGGACTACCTGGATATCAAGAAC
  TTTGGGGCCAAAGTGGTGGGCCTCTCCTGCACCCTGGCCTGTGGCAGCACCCTCTTCCTC
  GGCAAAGTGGGCCCTTTCGTGCACCTGTCTGTGATGATGGCTGCCTACCTGGGCCGTGTG
  CGCACCACGACCATCGGGGAGCCTGAGAACAAGAGCAAGCCTGTGTACTCCGCTCTGGCC
  ACCTTGGTTCTCGCCTCCATCACCTACCCACCCAGCGCCGGCCGCTTCCTAGCTTCTCGG
  CTGTCCATGAAGCAGCATCTGGACTCGCTGTTCGACAACCACTCCTGGGCGCTGATGACC
  CAGAACTCCAGCCCACCCTGGCCCGAGGAGCTCGACCCCCAGCACCTGTGGTGGGAATGG
  TACCACCCGCGGTTCACCATCTTTGGGACCCTTGCCTTCTTCCTGGTTATGAAGTTCTGG
  ATGCTGATTCTGGCCACCACCATCCCCATGCCTGCCGGGTACTTCATGCCCATCTTTGTC
  TATGGAGCTGCTATCGGGCGCCTCTTTGGGGAGACTCTCTCTTTTATCTTCCCTGAGGGC
  ATCGTGGCTGGAGGGATCACCAATCCCATCATGCCAGGGGGGTATGCTCTGGCAGGGGCT
  GCAGCCTTCTCAGGGGCTGTGACCCACACCATCTCCACGGCGCTGCTGGCCTTCGAGGTG
  ACCGGCCAGATAGTGCATGCACTGCCCGTGCTGATGGCGGTGCTGGCAGCCAACGCCATT
  GCACAGAGCTGCCAGCCCTCCTTCTATGATGGCACCGTCATTGTCAAGAAGCTGCCATAC
  CTGCCACGGATTCTGGGCCGCAACATCGGTTCCCACCGCGTGAGGGTGGAGCACTTCATG
  AACCACAGCATCACCACACTGGCCATCACATTAATGAATGATGAGATTGGAGTACACTGT
  CACCAAGGGCAGGCACAGATGGCTTCTGGGGTTGTCTGGTTCCCAGTGAGAGGCTCCTGA
  GAAAAATAAAGCTGGTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC020873 unedited
  NNNCCACTTTGTTCGCATTTGTGTACGACTCATATAGGCGGCCGCGTAATTCGCCATTAC
  GGCCGGGGGGGGGGAGGATGTTGATTGTTGGAACACACACCTGTCCAGGTTCAGGGGAGC
  TGGAGGCTCTGTGAGAGGAGGGCCAGCTCAGCCACAGCAGGAGGACTGACAGGGGCCTGA
  TGGAGGAGTTTGTGGGGCTGCGTGAAGGCTCCTCAGGGAACCCTGTGACTCTGCAGGAGC
  TGTGGGGCCCCTGTCCCCGCATCCGCCGAGGCATCCGAGGTGGCCTGGAGTGGCTGAAGC
  AGAAGCTCTTCCGCCTGGGCGAGGACTGGTACTTCCTGATGACCCTCGGGGTGCTCATGG
  CCCTGGTCAGCTGTGCCATGGACTTGGCTGTTGAGAGTGTGGTCCGAGCGCACCAGTGGC
  TGTACAGGGAGATTGGGGACAGCCACCTGCTCCGGTATCTCTCCTGGACTGTGTACCCTG
  TGGCCCTCGTCTCTTTCTCTTCGGGCTTCTCTCAGAGCATCACACCCTCCTCTGGAGGTT
  CTGGAATCCCGGAGGTGAAGACCATGTTGGCGGGTGTGGTCTTGGAGGACTACCTGGATA
  TCAAGAACTTTGGGGCCAAAGTGGTGGGCCTCTCCTGCACCCTGGCCTGTGGCAGCACCC
  TCTTCCTCGGCAAAGTGGGCCCTTTCGTGCACCTGTCTGTGATGATGGCTGCCTACCTGG
  GCCGTGTGCGCACCACGACCCATCGGGAGCCTGAGAACAAGAGCAAGCCTGTGTACTCCG
  CTCTGGCCCCTTGGTTCTCGCCTTCATCACCTACCCACCCAGCGCCGGCCGCTTCCTAGC
  TTCTCGGCTGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC020873 unedited
  GCATGTCACTTCCACGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGGGCCGAGGCGGCCG
  ACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGGAACCAGCTTTATTTTTCTCA
  GGAGCCTCTCACTGGGAACCAGACAACCCCAGAAGCCATCTGTGCCTGCCCTTGGTGACA
  GTGTACTCCAATCTCATCATTCATTAATGTGATGGCCAGTGTGGTGATGCTGTGGTTCAT
  GAAGTGCTCCACCCTCACGCGGTGGGAACCGATGTTGCGGCCCAGAATCCGTGGCAGGTA
  TGGCAGCTTCTTGAACAATGACGGTGCCATCATAGAAAGAGGGCTGGCAGCTCTGTGCAA
  TGGCGTTGGGCTGCCAGCCACNCGCCATCAGCACGGGCCAGTGCATTGCACTTATCTTGC
  CCGGGTCACCCTCGAAAGGGCCANCAANCGCCCGTTGGNAAAATGGGTGGTGGGGGTTCA
  CCAAGGCCCCCCTGTAAGAAAGGGGCTTGGCAAGCCCCCCCTTGCCCCAGGAGAGCATTT
  ACCCCCCCCCCTTGGGCCATTGTGATGGGGGGAATTTGGGGGTGGATTCCCCCTTTCCCA
  AGCCCCACCGGAATTGGCCCCTCCAGGGGGAGAGGAATATAAAAGGAAGAGGAGTTCTTC
  CCCCAAAAAGAAGGCCGCCCCGGATTAGCAAGCTTCCATAAGACAAAAGATGGGGCATGA
  AAGTACCCCGGCAGGCATGGGNGATGGTGGTTGCCAAGAATCAGCATCCAGAACTTCTAA
  CCCGGAAGAAGGCAAGGTCCCA
  
• Restriction Sites: Please inquire
• ACCN: BC020873
• Insert Size: 1552 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020873.1, AAH20873.1
• RefSeq Size: 1552 bp
• RefSeq ORF: 1386 bp
• Locus ID: 1188
• Cytogenetics: 1p36.13
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the family of voltage-gated chloride channels. Chloride channels have several functions, including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. This gene is expressed predominantly in the kidney and may be important for renal salt reabsorption. Mutations in this gene are associated with autosomal recessive Bartter syndrome type 3 (BS3). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'