CENPA (BC002703) Human Untagged Clone

CAT#: SC122235

CENPA (untagged)-Human centromere protein A, 17kDa (cDNA clone MGC:3892 IMAGE:3626578), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CENPA
• Synonyms: CENP-A; centromere protein A; centromere protein A, 17kDa; OTTHUMP00000122589
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC002703 edited
  GGCAGCCCGAGCAGGAGCCGTGGGACCGGGCGCCGGCACCCTCTGCGGCGTGTCATGGGC
  CCGCGCCGCCGGAGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCGACCCCG
  ACCCCCGGCCCCTCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACACAGTCGG
  CGGAGACAAGGTTGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTCTTGATA
  AGGAAGCTGCCCTTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGTGGTGTG
  GACTTCAATTGGCAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCATTTCTA
  GTTCATCTCTTTGAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTC
  CCAAAGGATGTGCAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCT
  CCTGCACCCAGTGTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGAC
  TCTCCAGAGCCATGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTC
  TGAACAGCTGGAGTGCAGCAGCACGATCTCGGCTCACTGCAACCTCCAACTCCCAAGTTC
  AAGCGATTCTCCTGCCTCAGCCTCTCGGACCCTGTCTCTATTTTTTTTTAAATAAAGAAA
  TAAAAATTCTGTGTAACTTCATAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC002703 unedited
  CAAAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGCAGCCCGAGC
  AGGAGCCGTGGGACCGGGCGCCGGCACCCTCTGCGGCGTGTCATGGGCCCGCGCCGCCGG
  AGCCGAAAGCCCGAGGCCCCGAGGAGGCGCAGCCCGAGCCCGACCCCGACCCCCGGCCCC
  TCCCGGCGGGGCCCCTCCTTAGGCGCTTCCTCCCATCAACACAGTCGGCGGAGACAAGGT
  TGGCTAAAGGAGATCCGAAAGCTTCAGAAGAGCACACACCTCTTGATAAGGAAGCTGCCC
  TTCAGCCGCCTGGCAAGAGAAATATGTGTTAAATTCACTCGTGGTGTGGACTTCAATTGG
  CAAGCCCAGGCCCTATTGGCCCTACAAGAGGCAGCAGAAGCATTTCTAGTTCATCTCTTT
  GAGGACGCCTATCTCCTCACCTTACATGCAGGCCGAGTTACTCTCTTCCCAAAGGATGTG
  CAACTGGCCCGGAGGATCCGGGGCCTTGAGGAGGGACTCGGCTGAGCTCCTGCACCCAGT
  GTTTCTGTCAGTCTTTCCTGCTCAGCCAGGGGGGATGATACCGGGGACTCTCCAGAGCCA
  TGACTAGATCCAATGGATTCTGCGATGCTGTCTGGACTTTGCTGTCTCTGAACAGCTGGA
  GTGCAGCAGCACGATCTCGGCTCACTGCAACCTCCAACTCCCAAGTTCAAGCGATTCTCC
  TGCCTCAGCCTCTCGGACCCTGTCTCTATTTTTTTTTAAATAAAGAAATAAAAATTCTGT
  GTAACTTCATAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGNT
  TCCCTGACAGATCCCGGNTGGCATCCCTGTGACCCTNCCCAGTGCCTCTCCTGGCCTTGG
  NAAGTGCACTCAGTGCCCACCAGCCTGTCTATAAAAATAGTGCACATTTGCTGCTAGTGT
  CTCTTAATAGGAGGGGGG
  
• Restriction Sites: Please inquire
• ACCN: BC002703
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002703.2, AAH02703.1
• RefSeq Size: 759 bp
• Locus ID: 1058
• Cytogenetics: 2p23.3
• Gene Summary: 'Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]'