U2AF65 (U2AF2) (BC008740) Human Untagged Clone

CAT#: SC122300

U2AF2 (untagged)-Human U2 (RNU2) small nuclear RNA auxiliary factor 2 (cDNA clone MGC:1210 IMAGE:3346087), complete cds


Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol U2AF2
Synonyms splicing factor U2AF 65 kD subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit; U2AF65
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for BC008740 edited
CTGGAGCGGGCGGCAAGGCGAGGCGAAAGCTGCACAGGGCCCTACGCGGCCGCCTCAGCA
TGTCGGACTTCGACGAGTTCGAGCGGCAGCTCAACGAGAATAAACAAGAGCGGGACAAGG
AGAACCGGCATCGGAAGCGCAGCCACAGCCGCTCTCGGAGCCGGGACCGCAAACGCCGGA
GCCGGAGCCGCGACCGGCGCAACCGGGACCAGCGGAGCGCCTCCCGGGACAGGCGACGAC
GCAGCAAACCTTTGACCAGAGGCGCTAAAGAGGAGCACGGTGGACTGATTCGTTCCCCCC
GCCACGAGAAGAAGAAGAAGGTCCGTAAATACTGGGACGTGCCACCCCCAGGCTTTGAGC
ACATCACCCCAATGCAGTACAAGGCCATGCAAGCTGCGGGTCAGATTCCAGCCACTGCTC
TTCTCCCCACCATGACCCCTGACGGTCTGGCTGTGACCCCAACGCCGGTGCCCGTGGTCG
GGAGCCAGATGACCAGACAAGCCCGGCGCCTCTACGTGGGCAACATCCCCTTTGGCATCA
CTGAGGAGGCCATGATGGATTTCTTCAACGCCCAGATGCGCCTGGGGGGGCTGACCCAGG
CCCCTGGCAACCCAGTGTTGGCTGTGCAGATTAACCAGGACAAGAATTTTGCCTTTTTGG
AGTTCCGCTCAGTGGACGAGACTACCCAGGCTATGGCCTTTGATGGCATCATCTTCCAGG
GCCAGTCACTAAAGATCCGCAGGCCTCACGACTACCAGCCGCTTCCTGGCATGTCAGAGA
ACCCCTCCGTCTATGTGCCTGGGGTTGTGTCCACTGTGGTCCCCGACTCTGCCCACAAGC
TGTTCATCGGGGGCTTACCCAACTACCTGAACGATGACCAGGTCAAAGAGCTGCTGACAT
CCTTTGGGCCCCTCAAGGCCTTCAACCTGGTCAAGGACAGTGCCACGGGGCTCTCCAAGG
GCTACGCCTTCTGTGAGTACGTGGACATCAACGTCACGGATCAGGCCATTGCGGGGCTGA
ACGGCATGCAGCTGGGGGATAAGAAGCTGCTGGTCCAGAGGGCGAGTGTGGGAGCCAAGA
ATGCCACGCTGAGCACCATCAATCAGACGCCTGTGACCCTGCAAGTGCCGGGCTTGATGA
GCTCCCAGGTGCAGATGGGCGGCCACCCGACTGAGGTCCTGTGCCTCATGAACATGGTGC
TGCCTGAGGAGCTGCTGGACGACGAGGAGTATGAGGAGATCGTGGAGGATGTGCGGGACG
AGTGCAGCAAGTACGGGCTTGTCAAGTCCATCGAGATCCCCCGGCCTGTGGACGGCGTCG
AGGTGCCCGGCTGCGGAAAGATCTTTGTGGAGTTCACCTCTGTGTTTGACTGCCAGAAAG
CCATGCAGGGCCTGACGGGCCGCAAGTTCGCCAACAGAGTGGTTGTCACAAAATACTGTG
ACCCCGACTCTTATCACCGCCGGGACTTCTGGTAGAGGCGGCTGGGGGAGGGTGGGGGCA
GGGCTGGCTGGGGGCTTCTCCCCACTCCCGCCCCCCCCTTATCCCCCTCTGAAGACGATG
GGCAGAGGAGTGACAGCCGCAGACACACGACAGCCGGCAGCAACTGGAATGGCAGCAATT
AAGGGTGGGGGGCGGGGGTTGGGGGGTTGGGGGGTTAGGGCAGGGAGGGGACTGGGGAAG
TGCGCACACAGCCCACACAGACAACACGCACCCACACAGACACAGAGGGAAGGGGTTGGG
ATGGGGACAGGGTGCACAGCAGGGCGGGGTAGGACCCCAGCCCCTCCCAAAACAGCCTCT
CCTTCTCCCATAGACCCCTTTCTTCTCCCCTTCCCCACGGTAGGAACATAGCGTGTTTAT
ATTTTATGGCCAAACTATTTTGAATTTTGTTGTCCGGCCCTCAGTGCCCTGCCCTCTCCC
TTACCAGGACCACAGCTCTGTTCCTTCGGCCTCTGGTCCTCTCTGGTCCCCTCCTGGGTT
TCTTACGTAGTTGATTTTTCCTCTTTAGTCTCCCCCGACCTGCGCCCAGCCCCGTGGCCC
CTGCCCCTCTCCTACTCTCTGTGGCAGTTTCATATTTGCTAAGACGAATTTGCTCATTAA
ACATTTTGTTGTATTTTACTTTAAAAAAAAAAAAAAAAAA
>OriGene 5' read for BC008740 unedited
NGCGCCGGAATTGTATACGACTTATATAGGCGGCACGCGAATTCGCACGAGGCTGGAGCG
GGCGGCAAGGCGAGGCGAAAGCTGCACATGGCCCTACGCGGCCGCCTCAGCATGTCGGAC
TTCGACGAGTTCGAGCGGCAGCTCAACGAGAATAAACAAGAGCGGGACAAGGAGAACCGG
CATCGGAAGCGCAGCCACAGCCGCTCTCGGAGCCGGGACCGCAAACGCCGGAGCCGGAGC
CGCGACCGGCGCAACCGGGACCAGCGGAGCGCCTCCCGGGACAGGCGACGACGCAGCAAA
CCTTTGACCAGAGGCGCTAAAGAGGAGCACGGTGGACTGATTCGTTCCCCCCGCCACGAG
AAGAAGAAGAAGGTCCGTAAATACTGGGACGTGCCACCCCCAGGCTTTGAGCACATCACC
CCAATGCAGTACAAGGCCATGCAAGCTGCGGGTCAGATTCCAGCCACTGCTCTTCTCCCC
ACCATGACCCCTGACGGTCTGGCTGTGACCCCAACGCCGGTGCCCGTGGTCGGGAGCCAG
ATGACCAGACAAGCCCGGCGCCTCTACGTGGGCAACATCCCCTTTGGCATCACTGAGGAG
GCCATGATGGATTTCTTCAACGCCCAGATGCGCCTGGGGGGGCTGACCCAGGCCCCTGGC
AACCCAGTGTTGGCTGTGCAGATTAACCAGGACAAGAATTTTGCCTTTTTGGAGTTCCGC
TCAGTGGACGAGACTACCCAGGCTATGGCCTTTGATGGCATCATCTTCCAGGGCCAGTCA
CTAAAGATCCCGCAGCCTCACGACTACCAGCCGCTTCCTGGCATGTCAGAGAACCCCTCC
GTCTATGTGCCTGGGGTTGTGTCCACTGTGGGTCCCGACTCTGCCCACNAGCTGNTCATC
GGGGGC
Restriction Sites Please inquire     
ACCN BC008740
Insert Size 2149
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Reference Data
RefSeq BC008740.2, AAH08740.1
RefSeq Size 2140
Locus ID 11338
Protein Pathways Spliceosome
Gene Summary U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]

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