SFTPB (NM_000542) Human Untagged Clone

CAT#: SC122565

SFTPB (untagged)-Human surfactant protein B (SFTPB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFTPB
• Synonyms: PSP-B; SFTB3; SFTP3; SMDP1; SP-B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000542 edited
  GCCATGGCTGAGTCACACCTGCTGCAGTGGCTGCTGCTGCTGCTGCCCACGCTCTGTGGC
  CCAGGCACTGCTGCCTGGACCACCTCATCCTTGGCCTGTGCCCAGGGCCCTGAGTTCTGG
  TGCCAAAGCCTGGAGCAAGCATTGCAGTGCAGAGCCCTAGGGCATTGCCTACAGGAAGTC
  TGGGGACATGTGGGAGCCGATGACCTATGCCAAGAGTGTGAGGACATCGTCCACATCCTT
  AACAAGATGGCCAAGGAGGCCATTTTCCAGGACACGATGAGGAAGTTCCTGGAGCAGGAG
  TGCAACGTCCTCCCCTTGAAGCTGCTCATGCCCCAGTGCAACCAAGTGCTTGACGACTAC
  TTCCCCCTGGTCATCGACTACTTCCAGAACCAGACTGACTCAAACGGCATCTGTATGCAC
  CTGGGCCTGTGCAAATCCCGGCAGCCAGAGCCAGAGCAGGAGCCAGGGATGTCAGACCCC
  CTGCCCAAACCTCTGCGGGACCCTCTGCCAGACCCTCTGCTGGACAAGCTCGTCCTCCCT
  GTGCTGCCCGGGGCCCTCCAGGCGAGGCCTGGGCCTCACACACAGGATCTCTCCGAGCAG
  CAATTCCCCATTCCTCTCCCCTATTGCTGGCTCTGCAGGGCTCTGATCAAGCGGATCCAA
  GCCATGATTCCCAAGGGTGCGCTAGCTGTGGCAGTGGCCCAGGTGTGCCGCGTGGTACCT
  CTGGTGGCGGGCGGCATCTGCCAGTGCCTGGCTGAGCGCTACTCCGTCATCCTGCTCGAC
  ACGCTGCTGGGCCGCATGCTGCCCCAGCTGGTCTGCCGCCTCGTCCTCCGGTGCTCCATG
  GATGACAGCGCTGGCCCAAGGTCGCCGACAGGAGAATGGCTGCCGCGAGACTCTGAGTGC
  CACCTCTGCATGTCCGTGACCACCCAGGCCGGGAACAGCAGCGAGCAGGCCATACCACAG
  GCAATGCTCCAGGCCTGTGTTGGCTCCTGGCTGGACAGGGAAAAGTGCAAGCAATTTGTG
  GAGCAGCACACGCCCCAGCTGCTGACCCTGGTGCCCAGGGGCTGGGATGCCCACACCACC
  TGCCAGGCCCTCGGGGTGTGTGGGACCATGTCCAGCCCTCTCCAGTGTATCCACAGCCCC
  GACCTTTGATGAGAACTCAGCTGTCCAGCTGCAAAGGAAAAGCCAAGTGAGACGGGCTCT
  GGGACCATGGTGACCAGGCTCTTCCCCTGCTCCCTGGCCCTCGCCAGCTGCCAGGCTGAA
  AAGAAGCCTCAGCTCCCACACCGCCCTCCTCACCGCCCTTCCTCGGCAGTCACTTCCACT
  GGTGGACCACGGGCCCCCAGCCCTGTGTCGGCCTTGTCTGTCTCAGCTCAACCACAGTCT
  GACACCAGAGCCCACTTCCATCCTCTCTGGTGTGAGGCACAGCGAGGGCAGCATCTGGAG
  GAGCTCTGCAGCCTCCACACCTACCACGACCTCCCAGGGCTGGGCTCAGGAAAAACCAGC
  CACTGCTTTACAGGACAGGGGGTTGAAGCTGAGCCCCGCCTCACACCCACCCCCATGCAC
  TCAAAGATTGGATTTTACAGCTACTTGCAATTCAAAATTCAGAAGAATAAAAAATGGGAA
  CATACAGAACTCTAAAAGATAGACATCAGAAATTGTTAAGTTAAGCTTTTTCAAAAAATC
  AGCAATTCCCCAGCGTAGTCAAGGGTGGACACTGCACGCTCTGGCATGATGGGATGGCGA
  CCGGGCAAGCTTTCTTCCTCGAGATGCTCTGCTGCTTGAGAGCTATTGCTTTGTTAAGAT
  ATAAAAAGGGGTTTCTTTTTGTCTTTCTGTAAGGTGGACTTCCAGCTTTTGATTGAAAGT
  CCTAGGGTGATTCTATTTCTGCTGTGATTTATCTGCTGAAAGCTCAGCTGGGGTTGTGCA
  AGCTAGGGACCCATTCCTGTGTAATACAATGTCTGCACCAATGCTAATAAAGTCCTATTC
  TCTTTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000542 unedited
  GCGCTCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCCCGGGATGCCATG
  GCTGAGTACACCTGCTGCAGTGGCTGCTGCTGCTGCTGCCCACGCTCTGTGGCCCAGGCA
  CTGCTGCCTGGACCACCTCATCCTTGGCCTGTGCCCAGGGCCCTGAGTTCTGGTGCCAAA
  GCCTGGAGCAAGCATTGCAGTGCAGAGCCCTAGGGCATTGCCTACAGGAAGTCTGGGGAC
  ATGTGGGAGCCGATGACCTATGCCAAGAGTGTGAGGACATCGTCCACATCCTTAACAAGA
  TGGCCAAGGAGGCCATTTTCCAGGACACGATGAGGAAGTTCCTGGAGCAGGAGTGCAACG
  TCCTCCCCTTGAAGCTGCTCATGCCCCAGTGCAACCAAGTGCTTGACGACTACTTCCCCC
  TGGTCATCGACTACTTCCAGAACCAGACTGACTCAAACGGCATCTGTATGCACCTGGGCC
  TGTGCAAATCCCGGCAGCCAGAGCCAGAGCAGGAGCCAGGGATGTCAGACCCCCTGCCCA
  AACCTCTGCGGGACCCTCTGCCAGACCCTCTGCTGGACAAGCTCGTCCTCCCTGTGCTGC
  CCGGGGCCCTCCAGGCGAGGCCTGGGCCTCACACACAGGATCTCTCCGAGCAGCAATTCC
  CCATTCCTCTCCCCTATTGCTGGCTCTGCAGGGCTCTGATCAAGCGGATCCAAGCCATGA
  TTCCCAAGGGTGCGCTAGCTGTGGCAGTGGCCCANGTGTGCCGCGTGGTACCTCTGGTGG
  CGGGCGGCATCTGCCAGTGCCTGGCTGAGCGCTACTCCGTCATCCTGCTCGACACGCTGC
  TGGGCCGCATGCTGCCCCAGCTGGTCTGCCGCCTCGTCCTCCGGGTGCTCATGGATGACA
  GCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_000542
• Insert Size: 2026 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000542.2, NP_000533.2
• RefSeq Size: 2026 bp
• RefSeq ORF: 1146 bp
• Locus ID: 6439
• Cytogenetics: 2p11.2
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]'
  Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein.