DIO3 (NM_001362) Human Untagged Clone

CAT#: SC122591

DIO3 (untagged)-Human deiodinase, iodothyronine, type III (DIO3) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Human Untagged Clone
• Symbol: DIO3
• Synonyms: 5DIII; D3; DIOIII; TXDI3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001362 edited
  CGCCAGGCCACGTCGCGGTTGGTGGTCGGAGAGGGCGAGGGGTCCCAGGGGGCTTCGGGG
  CCTGCAGCCACCATGCTCCGCTCCCTGCTGCTTCACTCCTTGAGGCTCTGCGCCCAGACC
  GCCTCGTGCCTCGTGCTCTTCCCGCGCTTCCTCGGCACGGCCTTCATGCTCTGGCTTCTC
  GATTTCTTGTGTATCCGCAAGCATTTCCTGGGCCGCCGCCGCCGGGGGCAGCCCGAGCCC
  GAAGTGGAGCTCAACAGTGAAGGCGAGGAGGTGCCTCCCGATGACCCGCCCATCTGCGTG
  TCCGACGACAACCGCCTGTGCACCCTGGCGTCGCTCAAGGCGGTGTGGCATGGCCAGAAG
  TTGGATTTCTTCAAGCAGGCGCACGAGGGCGGTCCGGCGCCCAACTCCGAGGTGGTTCTG
  CCCGACGGCTTCCAGAGCCAGCACATCCTCGACTACGCGCAAGGGAACCGCCCGCTGGTT
  CTCAATTTCGGCAGCTGCACCTGACCACCGTTCATGGCGCGCATGAGCGCCTTCCAGCGC
  CTGGTCACTAAGTACCAGCGCGACGTCGACTTCCTCATCATCTACATCGAGGAAGCGCAC
  CCCTCCGACGGCTGGGTCACCACGGACTCTCCCTACATCATCCCACAGCACCGGAGCCTG
  GAGGACCGGGTCAGCGCAGCGAGGGTACTGCAGCAAGGTGCACCCGGCTGCGCTCTGGTC
  CTCGACACCATGGCCAACTCCAGCAGCTCGGCCTATGGCGCCTACTTCGAGCGTCTCTAT
  GTCATCCAGAGTGGCACTATTATGTACCAGGGCGGCCGTGGCCCCGACGGCTACCAGGTC
  TCTGAGCTGCGCACTTGGTTGGAACGCTATGATGAGCAACTGCACGGCGCTCGGCCCCGG
  AGGGTGTAAACATCCAACGGACAATTGACTGAACTTGGTGGGCTGGGCCTTCGAGCCTTC
  GAAGCCCACGTGCAAGCGCCTCAAACCAAGTCACGCTTGGCGAGGCCCCAGTGACACTGA
  TGTGCTGAGCCACCATTTCAGACTGAGTCTGCACCCTCAGCCACATGAACAATCTCCCCT
  ACCTCCCTGGGACTCTGCTTCTGTAACTGTCTCATTCACACCTGCCTGGCTCACTGGAAA
  TCCTCTTTTGAGCGCGGGATATGGCTTGCCCTTGTCCGTGTGCCCCCAGGACTTTGCCTC
  TACAGCATTTTCTTACACCCCCTCCCCAGCGTGCCCTCAGCCAAGTGCTTTGGCCCGGTG
  CTTCCCGCAGCTGCACAGAGACCTTGGCCACGCCCGCGCGCCCTGAGCGCAGCTGGGTTC
  CAGGAGACTCTCAGCTCAGCTGAGCTAGTTGCCTGGCACCCACCTGTCGCGCGCGGAGAG
  GGGGTTCCCTGTTGCTTTTGTGTCTGTTTCCTGTCCCTGGTAGGGGAAGTGATGTCGTGG
  ATGGGGAGGGGTGGGCAGGGTAGTTTCCCCCGCTTGTTTTGGGTGCACAGGAGCCCCACT
  GCTGATGACGAACTATCTCTAACTGGTCTTGACCACGAGCTAGTTCTGAATTGCAGGGGC
  CTCAAAGCAGCACCTAAACCTTGAGGGGGAGGGTGCTCTGGGTTTCCGTGAGGTAACCAC
  CTTAAATGGGAGGGAAGTTGGGGTGTCTGCTTTGGGACCAGAGGAAGATAGCTTGAGAGG
  CATTGGCGAGGTTCGCAGCGCCCCAGGGAGAGAGAAAAAGCTGAGACTCCTGGGGAATGA
  CGTTGGGGTGATGGAGTCCGGGGAAAGAGAGGTGGGGGGAGAGCCTGAGGTCCCCAAGTG
  AGGGGAGTCCTAGGCAGAGCTGCTGATTGTGGGGCTGGGAGGTGGAGGGCCCCTGATTCG
  AAGGCCATTTGGTGAGTGTTTTGCTGGAAATATTTCCTGTATATAAACTTCTTTCAATCT
  ACAATAATAAAGGCTTGAGGTAAACTGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001362 unedited
  NGGGGATAGGATTTGTATACGACTTATATAGGCGGCCGCGCATTCCCGGGATATCGTCGA
  CCCACGCGTCCGCGCCAGGCCACGTCGCGGTTGGTGGTCGGAGAGGGCGAGGGGTCCCAG
  GGGGCTTCGGGGCCTGCAGCCACCATGCTCCGCTCCCTGCTGCTTCACTCCTTGAGGCTC
  TGCGCCCAGACCGCCTCGTGCCTCGTGCTCTTCCCGCGCTTCCTCGGCACGGCCTTCATG
  CTCTGGCTTCTCGATTTCTTGTGTATCCGCAAGCATTTCCTGGGCCGCCGCCGCCGGGGG
  CAGCCCGAGCCCGAAGTGGAGCTCAACAGTGAAGGCGAGGAGGTGCCTCCCGATGACCCG
  CCCATCTGCGTGTCCGACGACAACCGCCTGTGCACCCTGGCGTCGCTCAAGGCGGTGTGG
  CATGGCCAGAAGTTGGATTTCTTCAAGCAGGCGCACGAGGGCGGTCCGGCGCCCAACTCC
  GAGGTGGTTCTGCCCGACGGCTTCCAGAGCCAGCACATCCTCGACTACGCGCAAGGGAAC
  CGCCCGCTGGTTCTCAATTTCGGCAGCTGCACCTGACCACCGTTCATGGCGCGCATGAGC
  GCCTTCCAGCGCCTGGTCACTAAGTACCAGCGCGACGTCGACTTCCTCATCATCTACATC
  GAGGAAGCGCACCCCTCCGACGGCTGGGTCACCACGGACTCTCCCTACATCATCCCACAG
  CACCGGAGCCTGGAGGACCGGGTCAGCGCAGCGAGGGTACTGCAGCAAGGTGCACCCGGC
  TGCGCTCTGGTCCTCGACACCATGGCCAACTCCAGCAGCTCGGCCTATGGCGCCTACTTC
  GAGCGTCTCTATGTCATCCAGAGTGGCACTATTATGTACCCAGGCGGCCGTGGCCCCGAC
  GGCTN
  
• Restriction Sites: Please inquire
• ACCN: NM_001362
• Insert Size: 2098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001362.2, NP_001353.3
• RefSeq Size: 2120 bp
• Locus ID: 1735
• Cytogenetics: 14q32.31
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, May 2016]'