Nkx2.5 (NKX2-5) (NM_004387) Human Untagged Clone

CAT#: SC122678

NKX2 (untagged)-Human NK2 transcription factor related, locus 5 (Drosophila) (NKX2-5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NKX2-5
• Synonyms: CHNG5; CSX; CSX1; HLHS2; NKX2.5; NKX2E; NKX4-1; VSD3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004387 edited
  GACGGGTGCGCGGGCGGGCGGCGGCACCATGCAGGGAAGCTGCCAGGGGCCGTGGGCAGC
  GCCGCTTTCTGCCGCCCACCTGGCGCTGTGAGACTGGCGCTGCCACCATGTTCCCCAGCC
  CTGCTCTCACGCCCACGCCCTTCTCAGTCAAAGACATCCTAAACCTGGAACAGCAGCAGC
  GCAGCCTGGCTGCCGCCGGAGAGCTCTCTGCCCGCCTGGAGGCGACCCTGGCGCCCTCCT
  CCTGCATGCTGGCCGCCTTCAAGCCAGAGGCCTACGCTGGGCCCGAGGCGGCTGCGCCGG
  GCCTCCCAGAGCTGCGCGCAGAGCTGGGCCGCGCGCCTTCACCGGCCAAGTGTGCGTCTG
  CCTTTCCCGCCGCCCCCGCCTTCTATCCACGTGCCTACAGCGACCCCGACCCAGCCAAGG
  ACCCTAGAGCCGAAAAGAAAGAGCTGTGCGCGCTGCAGAAGGCGGTGGAGCTGGAGAAGA
  CAGAGGCGGACAACGCGGAGCGGCCCCGGGCGCGACGGCGGAGGAAGCCGCGCGTGCTCT
  TCTCGCAGGCGCAGGTCTATGAGCTGGAGCGGCGCTTCAAGCAGCAGCGGTACCTGTCGG
  CCCCCGAACGCGACCAGCTGGCCAGCGTGCTGAAACTCACGTCCACGCAGGTCAAGATCT
  GGTTCCAGAACCGGCGCTACAAGTGCAAGCGGCAGCGGCAGGACCAGACTCTGGAGCTGG
  TGGGGCTGCCCCCGCCGCCGCCGCCGCCTGCCCGCAGGATCGCGGTGCCAGTGCTGGTGC
  GCGATGGCAAGCCATGCCTAGGGGACTCGGCGCCCTACGCGCCTGCCTACGGCGTGGGCC
  TCAATCCCTACGGTTATAACGCCTACCCCGCCTATCCGGGTTACGGCGGCGCGGCCTGCA
  GCCCTGGCTACAGCTGCACTGCCGCTTACCCCGCCGGGCCTTCCCCAGCGCAGCCGGCCA
  CTGCCGCCGCCAACAACAACTTCGTGAACTTCGGCGTCGGGGACTTGAATGCGGTTCAGA
  GCCCCGGGATTCCGCAGAGCAACTCGGGAGTGTCCACGCTGCATGGTATCCGAGCCTGGT
  AGGGAAGGGACCCGCGTGGCGCGACCCTGACCGATCCCACCTCAACAGCTCCCTGACTCT
  CGGGGGGAGAAGGGGCTCCCAACATGACCCTGAGTCCCCTGGATTTTGCATTCACTCCTG
  CGGAGACCTAGGAACTTTTTCTGTCCCACGCGCGTTTGTTCTTGCGCACGGGAGAGTTTG
  TGGCGGCGATTATGCAGCGTGCAATGAGTGATCCTGCAGCCTGGTGTCTTAGCTGTCCCC
  CCAGGAGTGCCCTCCGAGAGTCCATGGGCACCCCCGGTTGGAACTGGGACTGAGCTCGGG
  CACGCAGGGCCTGAGATCTGGCCGCCCATTCCGCGAGCCAGGGCCGGGCGCCCGGGCCTT
  TGCTATCTCGCCGTCGCCCGCCCACGCACCCACCCGTATTTATGTTTTTACCTATTGCTG
  TAAGAAATGACGATCCCCTTCCCATTAAAGAGAGTGCGTTGAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004387 unedited
  NGCGGTTCAGATTTTGTAATACGACTTATTATAGGGCGGCCGCGATTCCCGGGATGACGG
  GTGCGCGGGCGGGCGGCGGCACCATGCAGGGAAGCTGCCAGGGGCCGTGGGCAGCGCCGC
  TTTCTGCCGCCCACCTGGCGCTGTGAGACTGGCGCTGCCACCATGTTCCCCAGCCCTGCT
  CTCACGCCCACGCCCTTCTCAGTCAAAGACATCCTAAACCTGGAACAGCAGCAGCGCAGC
  CTGGCTGCCGCCGGAGAGCTCTCTGCCCGCCTGGAGGCGACCCTGGCGCCCTCCTCCTGC
  ATGCTGGCCGCCTTCAAGCCAGAGGCCTACGCTGGGCCCGAGGCGGCTGCGCCGGGCCTC
  CCAGAGCTGCGCGCAGAGCTGGGCCGCGCGCCTTCACCGGCCAAGTGTGCGTCTGCCTTT
  CCCGCCGCCCCCGCCTTCTATCCACGTGCCTACAGCGACCCCGACCCAGCCAAGGACCCT
  AGAGCCGAAAAGAAAGAGCTGTGCGCGCTGCAGAAGGCGGTGGAGCTGGAGAAGACAGAG
  GCGGACAACGCGGAGCGGCCCCGGGCGCGACGGCGGAGGAAGCCGCGCGTGCTCTTCTCG
  CAGGCGCANGTCTATGAGCTGGAGCGGCGCTTCAAGCAGCAGCGGTACCTGTCGGCCCCC
  GAACGCGACCAGCTGGCCAGCGTGCTGAAACTCACGTCCACGCAGGTCAAGATCTGGTTC
  CAGAACCCGCGCTANCAGTGCAAGCGGCAGCGGCAGGACCAGACTCTGGAGCTGGGGGGG
  CTGCCCCGNCGGCGCGGCGCCTGCCCGNAGATCGCGGTGCAGTGCTGTGCGCGATGGCAG
  CCATGCCTAGGGACTCGGCGCCTACCGCCTGCTACGCGTGGGCCTCAATCCTACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004387 unedited
  GGACATTCTTCCCCTTTATAGGGNAAGGGTTCCGTNCATTTCTTACAGCAATAGGGTAAA
  AACATAAATACGGGTGGGTGCGTGGGCGGGCGACGGCGAAGAAGCAAAGGCCCGGGCGCC
  CGGCCCTGGCTCGCGGAATGGGCGGCCAGATCTCAGGCCCTGCGTGCCCGAGCTCAGTCC
  CAGTTCCAACCGGGGGTGCCCATGGACTCTCGGAGGGCACTCCTGGGGGGACAGCTAAGA
  CACCAGGCTGCAGGATCACTCATTGCACGCTGCATAATCGCCGCCACAAACTCTCCCGTG
  CGCAAGAACAAACGCGCGTGGGACAGAAAAAGTTCCTAGGTCTCCGCAGGAGTGAATGCA
  AAATCCAGGGGACTCAGGGTCATGTTGGGAGCCCCTTCTCCCCCCGAGAGTCAGGGAGCT
  GTTGAGGTGGGATCGGTCAGGGTCGCGCCACGCGGGTCCCTTCCCTACCAGGCTCGGATA
  CCATGCAGCGTGGACACTCCCGAGTTGCTCTGCGGAATCCCGGGGCTCTGAACCGCATTC
  AAGTCCCCGACGCCGAAGTTCACGAAGTTGTTGTTGGCGGCGGCAGTGGCCGGCTGCGCT
  GGGGAAGGCCCGGCGGGGTAAGCGGCAGTGCAGCTGTAGCCAGGGCTGCAGGCCGCGCCG
  CCGTAACCCGGATAGGCGGGGTAGGCGTTATAACCGTAGGGATTGAGGCCCACGCCGTAA
  GCAGGCGCGTAGGGCGCCGAGTCCCCTAGGCATGGCTTGCCATCGCGCACAACACTGGCA
  CCGCGATCCTGCGGCAAGCGGCGGCCGCCGGCGGGGCAACCCCCCCCGCTCCAGAATCTG
  GTCCTGGCGCTGCCGCTTGACTTGTACGCCCGGTTCTGGACCAGATCTTGACCCGCGTGG
  ACCTGAATTTAACAAACTGGCCACTTGGTG
  
• Restriction Sites: Please inquire
• ACCN: NM_004387
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004387.2, NP_004378.1
• RefSeq Size: 1585 bp
• RefSeq ORF: 975 bp
• Locus ID: 1482
• Cytogenetics: 5q35.1
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (1) represents the shortest transcript but encodes the longest isoform (1).