KCNJ1 (NM_153767) Human Untagged Clone

CAT#: SC123939

KCNJ1 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), transcript variant rom-k5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ1
• Synonyms: KIR1.1; ROMK; ROMK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153767, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCAAACATCTTCGGAAATGGGTCGTCACTCGCTTTTTTGGGCATTCTCGGCAAAGAGCAAGGCTAG
  TCTCCAAAGATGGAAGGTGCAACATAGAATTTGGCAATGTGGAGGCACAGTCAAGGTTTATATTCTTTGT
  GGACATCTGGACAACGGTACTTGACCTCAAGTGGAGATACAAAATGACCATTTTCATCACAGCCTTCTTG
  GGGAGTTGGTTTTTCTTTGGTCTCCTGTGGTATGCAGTAGCGTACATTCACAAAGACCTCCCGGAATTCC
  ATCCTTCTGCCAATCACACTCCCTGTGTGGAGAATATTAATGGCTTGACCTCAGCTTTTCTGTTTTCTCT
  GGAGACTCAAGTGACCATTGGATATGGATTCAGGTGTGTGACAGAACAGTGTGCCACTGCCATTTTTCTG
  CTTATCTTTCAGTCTATACTTGGAGTTATAATCAATTCTTTCATGTGTGGGGCCATCTTAGCCAAGATCT
  CCAGGCCCAAAAAACGTGCCAAGACCATTACGTTCAGCAAGAACGCAGTGATCAGCAAACGGGGAGGGAA
  GCTTTGCCTCCTAATCCGAGTGGCTAATCTCAGGAAGAGCCTTCTTATTGGCAGTCACATTTATGGAAAG
  CTTCTGAAGACCACAGTCACTCCTGAAGGAGAGACCATTATTTTGGACCAGATCAATATCAACTTTGTAG
  TTGACGCTGGGAATGAAAATTTATTCTTCATCTCCCCATTGACAATTTACCATGTCATTGATCACAACAG
  CCCTTTCTTCCACATGGCAGCGGAGACCCTTCTCCAGCAGGACTTTGAATTAGTGGTGTTTTTAGATGGC
  ACAGTGGAGTCCACCAGTGCTACCTGCCAAGTCCGGACATCCTATGTCCCAGAGGAGGTGCTTTGGGGCT
  ACCGTTTTGCTCCCATAGTATCCAAGACAAAGGAAGGGAAATACCGAGTGGATTTCCATAACTTTAGCAA
  GACAGTGGAAGTGGAGACCCCTCACTGTGCCATGTGCCTTTATAATGAGAAAGATGTTAGAGCCAGGATG
  AAGAGAGGCTATGACAACCCCAACTTCATCTTGTCAGAAGTCAATGAAACAGATGACACCAAAATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153767 unedited
  GCATTCGGCACCAGCCGGTCTGAGCTGGCCCACAGACTCATAAAATCAACAGGGCCTCGG
  GTACCCTCACCCAGCATATCCAAACTCTTGCATCAAAGGTGCAGGGACTTGCTCACATCG
  AGAATCTGGTTGCTTTCTTGGAGACCAAGAAAATGAGTTTTTGTTTCTACATTTACTCCA
  GCAATCCATGAGGACTTTATAGGAATTTTGCACCATTCTGAATGGATACATTTGGATTTC
  TCAACATTTGTTCAGCTTCCTAATGACTGTTGTGACAATTGCTCTATACCAGTGAATGCC
  AACTGTTTATCTCTGCTCTGAACAGGTTTCTTCTCTACTGTGCCTTCATCCCCATCCTGG
  GCCCTGACAAAGACAGTGTGACAAGTTCTGAGCCCCACAGGTGGAATCCCAAACCAAGCA
  GCTCTTGCTGGTCACCCCAAGATCTACTTATACATGAAGTTTTGAAAATCATCTCCTGAA
  CCTCTTCTGACAGAGATCAGGGTGTTGACAGAAAGTATGTTCAAACATCTTCGGAAATGG
  GTCGTCACTCGCTTTTTTGGGCATTCTCGGCAAAGAGCAAGGCTAGTCTCCAAAGATGNA
  AGGTGCAACATAGAATTTGGAATGTGGAGCACAGTCAGGTTTATATTCTTGTGACATCTG
  GACACGGTACTGANCTAAGTGAGATACAAATGACATTTTATCACAGCTCTTGGAGAGTGT
  TTTCTTGTCTCCGGTATGAATACGTACATCCAAGACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153767 unedited
  GGTGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA
  TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTGGTT
  TTTTTTTTTTTTTTTTTTTTTTCCACAGTTTCAGAAATTTACTATTTTAATCAACACACT
  GAAAACATTTTGATTCCTAAGCAGCTTTGAGTACCAGTTTTTTTCCAGGACACATTCGGA
  ATGATGCAGGTGGGGATCTGAAAAAAACTTTCACAGTAAAACTTTCCCCTCAAGGTCGAG
  CCTTCCATAACCTGGCCCTTTTAAGAAAAATGGCTCTCCAGGAAAAGAAGACATAATAAC
  CTTTTGGCTGTGGGGGTCCATCCCATTTCTCTCTCTTTCCCACTTCTTCTTTAGAAATAG
  CATTCCAGTTCAAACTCTAGGGGGGGGGAACACATGAAACTGTTTCTTCTAGGTGCATTA
  ATGATACATTCCCTCTCTACTGACTTTGACCTTCCAAACTCCTCATTGCTGTCTTTGAAG
  AGCAGGGAAATGCTCTTTATAACCGGATTTAATGCCCCTTTAAGGCCTTCCAATTCCGAG
  TTGGAGGAAATTTTCTCCCCCCCTTTTAAATTAAAACTTTTCGGTCTTGGAGGAGGGTTT
  CCTTCAAGATTTCTTGGGGCATTTTTTACCTTGGGTTACCTTTCAACTTGAAAAGCTTAG
  GCCCCAATTTGGTCTTTGGGAAACCTTTTTGGGCCAGAAGGGGGTGTTTTCTCCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_153767
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153767.1, NP_722451.1
• RefSeq Size: 2749 bp
• RefSeq ORF: 1119 bp
• Locus ID: 3758
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5, also known as rom-k5) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter at the N-terminus than isoform a. Variants 2, 4 and 5 all encode isoform b but differ in their 5' UTRs.