KIR2.3 (KCNJ4) (NM_004981) Human Untagged Clone

CAT#: SC123960

KCNJ4 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 4 (KCNJ4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ4
• Synonyms: HIR; HIRK2; HRK1; IRK-3; IRK3; Kir2.3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004981, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCACGGACACAGCCGCAACGGCCAGGCCCACGTGCCCCGGCGGAAGCGCCGCAACCGCTTCGTCAAGA
  AGAACGGCCAATGCAACGTGTACTTCGCCAACCTGAGCAACAAGTCGCAGCGCTACATGGCGGACATCTT
  CACCACCTGCGTGGACACGCGCTGGCGCTACATGCTCATGATCTTCTCCGCGGCCTTCCTTGTCTCCTGG
  CTCTTTTTCGGCCTCCTCTTCTGGTGTATCGCCTTCTTCCACGGTGACCTGGAGGCCAGCCCAGGGGTGC
  CTGCGGCGGGGGGCCCGGCGGCGGGTGGTGGCGGAGCAGCCCCGGTGGCCCCCAAGCCCTGCATCATGCA
  CGTGAACGGCTTCCTGGGTGCCTTCCTGTTCTCGGTGGAGACGCAGACGACCATCGGCTATGGGTTCCGG
  TGCGTGACAGAGGAGTGCCCGCTGGCAGTCATCGCTGTGGTGGTCCAGTCCATCGTGGGCTGCGTCATCG
  ACTCCTTCATGATTGGCACCATCATGGCCAAGATGGCGCGGCCCAAGAAGCGGGCGCAGACGTTGCTGTT
  CAGCCACCACGCGGTCATTTCGGTGCGCGACGGCAAGCTCTGCCTCATGTGGCGCGTGGGCAACCTGCGC
  AAGAGCCACATTGTGGAGGCCCACGTGCGGGCCCAGCTCATCAAGCCCTACATGACCCAGGAGGGCGAGT
  ACCTGCCCCTGGACCAGCGGGACCTCAACGTGGGCTATGACATCGGCCTGGACCGCATCTTCCTGGTGTC
  GCCCATCATCATTGTCCACGAGATCGACGAGGACAGCCCGCTTTATGGCATGGGCAAGGAGGAGCTGGAG
  TCGGAGGACTTTGAGATCGTGGTCATCCTGGAGGGCATGGTGGAGGCCACGGCCATGACCACCCAGGCCC
  GCAGCTCCTACCTGGCCAGCGAGATCCTGTGGGGCCACCGCTTTGAGCCTGTGGTCTTCGAGGAGAAGAG
  CCACTACAAGGTGGACTACTCACGTTTTCACAAGACCTACGAGGTGGCCGGCACGCCCTGCTGCTCGGCC
  CGGGAGCTGCAGGAGAGTAAGATCACCGTGCTGCCCGCCCCACCGCCCCCTCCCAGTGCCTTCTGCTACG
  AGAACGAGCTGGCCCTTATGAGCCAGGAGGAAGAGGAGATGGAGGAGGAGGCAGCTGCGGCGGCCGCGGT
  GGCCGCAGGCCTGGGCCTGGAGGCGGGTTCCAAGGAGGAGGCGGGCATCATCCGGATGCTGGAGTTCGGC
  AGCCACCTGGACCTGGAGCGCATGCAGGCTTCCCTCCCGCTGGACAACATCTCCTACCGCAGGGAGTCTG
  CCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004981 unedited
  NGGAGTCAGGATTTGTAACCGACTTACTATAGGCGGCCGCGCAATCGGCACGAGCCTGAC
  TGTCACATTGGATTCTCCCCTCACCACTGCTCCTAACAAGTGTGGGCACTGGGCAGGAAT
  TTGGGCTCAAGGACTCAAAGCAACACTGGGAGAGAAGAGTCTAAAAAAGGAGCTCAGACC
  CAGGCCCTGCGTCTTCCCAGGTGACCACGCCGGCTTTTTGACATGCACGGACACAGCCGC
  AACGGCCAGGCCCACGTGCCCCGGCGGAAGCGCCGCAACCGCTTCGTCAAGAAGAACGGC
  CAATGCAACGTGTACTTCGCCAACCTGAGCAACAAGTCGCAGCGCTACATGGCGGACATC
  TTCACCACCTGCGTGGACACGCGCTGGCGCTACATGCTCATGATCTTCTCCGCGGCCTTC
  CTTGTCTCCTGGCTCTTTTTCGGCCTCCTCTTCTGGTGTATCGCCTTCTTCCACGGTGAC
  CTGGAGGCCAGCCCAGGGGTGCCTGCGGCGGGGGGCCCGGCGGCGGGTGGTGGCGGAGCA
  GCCCCGGTGGCCCCCAAGCCCTGCATCATGCACGTGAACGGCTTCCTGGGTGCCTTCCTG
  TTCTCGGTGGAGACGCAGACGACCATCGGCTATGGGTTCCGGTGCGTGACAGAGGAGTGC
  CCGCTGGCAGTCATCGCTGTGGTGGTCCAGTCCATCGTGGGCTGCGTCATCGACTCCTTC
  ATGATTGGCACCATCATGGCCAAGATGGCGCGGCCCAAGAAGCGGGCGCAGACGTTGCTG
  TTCAGCCACCACGCGGTCATTTCGGTGCGCGACGGCNAGCTCTGCCTCATGTGGCGCGTG
  GGCAACCTGCGCAAGAGCCACATTGTGGAGGCCCACCTGCGGGCCAGCTCATCAAGCCCT
  AA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004981 unedited
  NTTGCCTGCATGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
  TTTTTGTTTTTTTCCATCTGTCTCCATTTATAGATATTTACAAAAGAAAGTCATGAGCAA
  CAGGCCACAGTCATGCAAAAGAAACGCACCCCCCCTCCCCAAACTAACCCCCTGCCCACC
  CTGAAACCCCGAGATGTCCCCCGCCCAGCAGTCCAGCCACCTTCCCCAAGGTTCTGAGAG
  CAAAGAGGGCACGTCCTTGAAAAATCANTGGGGGAAGGGTGGTGGATCCGGGGACCTAGA
  GCCACCAAAAGTAGGCGCTGGCGGAACTCAGGCTGATCGGGGCCGAGCTCTTCCCAGGCC
  TGGGTTGCTGAGTCANNGAGAAGGGTCCCCCAGTCTTTGAACCCCCACTTTCTCAGAANG
  GTCACGACCCAGGTGGCTCTGTCTGAGTTGGGAGAGGTCTGCATCCACCCCGCGAGCTCT
  GTGGCATGTGAGGCCGGCTGAGTAATGCNACTCCTGCGAGAATGTGTCACGAGGAGCTGC
  TCCTCAGTCAGTGTGCGACTCACATCGATAGCCGCTCTNCTGACNGCTCAGCCAGCTGGG
  CACGGCGCGACTGTCTCTATTCTTCTCTGTAAAGCAATGTTCTCAAGCTGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_004981
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004981.1, NP_004972.1
• RefSeq Size: 1913 bp
• RefSeq ORF: 1338 bp
• Locus ID: 3761
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family. The encoded protein has a small unitary conductance compared to other members of this protein family. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.