Deoxyguanosine kinase (DGUOK) (NM_080918) Human Untagged Clone

CAT#: SC124293

DGUOK (untagged)-Human deoxyguanosine kinase (DGUOK), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGUOK
• Synonyms: dGK; MTDPS3; NCPH; PEOB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124293 sequence for NM_080918 edited (data generated by NextGen Sequencing)
  ATGGCCGCGGGCCGCCTCTTTCTAAGTCGGCTTCGAGCACCCTTCAGTTCCATGGCCAAG
  AGCCCACTCGAGGGCGTTTCCTCCTCCAGAGGCCTGCACGCGGGGCGCGGGCCCCGAAGG
  CTCTCCATCGAAGGCAACATTGCTGTGGGAAAGTCCACGTTTGTGAAGTTACTCACGAAA
  ACTTACCCAGAATGGCACGTAGCTACAGAACCTGTAGCAACATGGCAGAATATCCAGGCT
  GCTGGCACCCAAAAAGCCTGCACTGCCCAAAGTCTTGGAAACTTGCTGGATATGATGTAC
  CGGGAGCCAGCACGATGGTCCTACACATTCCAGACATTTTCCTTTTTGAGCCGCCTGAAA
  GTACAGCTGGAGCCCTTCCCTGAGAAACTCTTACAGGCCAGGAAGCCAGTACAGATCTTT
  GAGAGGTCTGTGTACAGTGACAGGCTCCACTTTGAGGCTCTGATGAACATTCCAGTGCTG
  GTGTTGGATGTCAATGATGATTTTTCTGAGGAAGTAACCAAACAAGAAGACCTCATGAGA
  GAGGTAAACACCTTTGTAAAGAATCTGTAA
  
  Clone variation with respect to NM_080918.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080918 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGATCGCTGTGTGAATCG
  TGGGTGGGATGGCCGCGGGCCGCCTCTTTCTAAGTCGGCTTCGAGCACCCTTCAGTTCCA
  TGGCCAAGAGCCCACTCGAGGGCGTTTCCTCCTCCAGAGGCCTGCACGCGGGGCGCGGGC
  CCCGAAGGCTCTCCATCGAAGGCAACATTGCTGTGGGAAAGTCCACGTTTGTGAAGTTAC
  TCACGAAAACTTACCCAGAATGGCACGTAGCTACAGAACCTGTAGCAACATGGCAGAATA
  TCCAGGCTGCTGGCACCCAAAAAGCCTGCACTGCCCAAAGTCTTGGAAACTTGCTGGATA
  TGATGTACCGGGAGCCAGCACGATGGTCCTACACATTCCAGACATTTTCCTTTTTGAGCC
  GCCTGAAAGTACAGCTGGAGCCCTTCCCTGAGAAACTCTTACAGGCCAGGAAGCCAGTAC
  AGATCTTTGAGAGGTCTGTGTACAGTGACAGGCTCCACTTTGAGGCTCTGATGAACATTC
  CAGTGCTGGTGTTGGATGTCAATGATGATTTTTCTGAAGAAGTAACCAAACAAGAAGACC
  TCATGAGAGAGGTAAACACCTTTGTGAAGAATCTGTAACCAATACCATGAAGTTCAGGCT
  GTGATCTGGGCTCCCTGACCTTCTGAAGCTAGAAAAATGGTGTGTCTTCCAACCACCCTT
  TCCATCCCAGCCCCTTTCATTCCTTGGAGCCACTCTGGCGCTCAAGAGCTGGGTTGGTAA
  TTATGGTTAGACCTTGCCATGGTTTCTTTTGTACCTGAAGCATTTTGAAATAAAGGTTAC
  CTCAAGTTATGCCTGGTTTTCTTACAAAAAAAAAAAAAAACTCGATCCTAAATTGCGGCC
  GCGGGCATAACTGTTTCCTGACACGATCCCGGGGGGCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080918
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080918.1, NP_550440.1
• RefSeq Size: 880 bp
• RefSeq ORF: 570 bp
• Locus ID: 1716
• Cytogenetics: 2p13.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Gene Summary: 'In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b)has the same N- and C-termini but is shorter compared to isoform 1.