CISH (NM_145071) Human Untagged Clone

CAT#: SC124560

CISH (untagged)-Human cytokine inducible SH2-containing protein (CISH), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CISH
• Synonyms: BACTS2; CIS; CIS-1; G18; SOCS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124560 sequence for NM_145071 edited (data generated by NextGen Sequencing)
  ATGGTCCTCTGCGTTCAGGGACCTCGTCCTTTGCTGGCTGTGGAGCGGACTGGGCAGCGG
  CCCCTGTGGGCCCCGTCCCTGGAACTGCCCAAGCCAGTCATGCAGCCCTTGCCTGCTGGG
  GCCTTCCTCGAGGAGGTGGCAGAGGGTACCCCAGCCCAGACAGAGAGTGAGCCAAAGGTG
  CTGGACCCAGAGGAGGATCTGCTGTGCATAGCCAAGACCTTCTCCTACCTTCGGGAATCT
  GGCTGGTATTGGGGTTCCATTACGGCCAGCGAGGCCCGACAACACCTGCAGAAGATGCCA
  GAAGGCACGTTCTTAGTACGTGACAGCACGCACCCCAGCTACCTGTTCACGCTGTCAGTG
  AAAACCACTCGTGGCCCCACCAATGTACGCATTGAGTATGCCGACTCCAGCTTCCGTCTG
  GACTCCAACTGCTTGTCCAGGCCACGCATCCTGGCCTTTCCGGATGTGGTCAGCCTTGTG
  CAGCACTATGTGGCCTCCTGCACTGCTGATACCCGAAGCGACAGCCCCGATCCTGCTCCC
  ACCCCGGCCCTGCCTATGCCTAAGGAGGATGCGCCTAGTGACCCAGCACTGCCTGCTCCT
  CCACCAGCCACTGCTGTACACCTAAAACTGGTGCAGCCCTTTGTACGCAGAAGCAGTGCC
  CGCAGCCTGCAACACCTGTGCCGCCTTGTCATCAACCGTCTGGTGGCCGACGTGGACTGC
  CTGCCACTGCCCCGGCGCATGGCCGACTACCTCCGACAGTACCCCTTCCAGCTCTGA
  
  Clone variation with respect to NM_145071.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145071 unedited
  AAANGGGGAAAACAAACCCCTCCCNCNGNCCGGTTCAAATTTGTATACGACTCCTATAGG
  CGGCCGCGNAATTCGCACGAGCGGNATCGCCGCTGCCGCGGGGACATGGTCCTCTGCGTT
  CAGGGACCTCGTCCTTTGCTGGCTGTGGAGCGGACTGGGCAGCGGCCCCTGTGGGCCCCG
  TCCCTGGAACTGCCCAAGCCAGTCATGCAGCCCTTGCCTGCTGGGGCCTTCCTCGAGGAG
  GTGGCAGAGGGTACCCCAGCCCAGACAGAGAGTGAGCCAAAGGTGCTGGACCCAGAGGAG
  GATCTGCTGTGCATAGCCAAGACCTTCTCCTACCTTCGGGAATCTGGCTGGTATTGGGGT
  TCCATTACGGCCAGCGAGGCCCGACAACACCTGCAGAAGATGCCAGAAGGCACGTTCTTA
  GTACGTGACAGCACGCACCCCAGCTACCTGTTCACGCTGTCAGTGAAAACCACTCGTGGC
  CCCACCAATGTACGCATTGAGTATGCCGACTCCAGCTTCCGTCTGGACTCCAACTGCTTG
  TCCAGGCCACGCATCCTGGCCTTTCCGGATGTGGTCAGCCTTGTGCAGCACTATGTGGCC
  TCCTGCACTGCTGATACCCGAAGCGACAGCCCCGATCCTGCTCCCACCCCGGCCCTGCCT
  ATGCCTAAGGANGATGCGCCTAGTGACCCAGCACTGCCTGCTCCTNCACCAGCCACTGCT
  GTACACCTAAAACTGGTGCAGCCCTTTGTACGCAGAAGCAGTGCCCGCAGCCCTGCACAC
  CTGTGCCGNCTTGTCATCAACCGTCTGGTGGCCGACGTGGACTGCCTGCCACTGGCCCGN
  NGCATGGGCGACTACCTNCGAAAGTACCCCTTNCAGCTTTGACTGTACGGGGACATTTGC
  CCACCCTTACCCAGNTGCACCCCTGGAGGGGAATCAACCCCAGCTGGACTGGGGNCCCCA
  CTGNCCTTCTCCAGCATNCTGNNGGCTGCATACCTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145071
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145071.1, NP_659508.1
• RefSeq Size: 2035 bp
• RefSeq ORF: 777 bp
• Locus ID: 1154
• Cytogenetics: 3p21.2
• Domains: SH2, SOCS
• Protein Families: Druggable Genome
• Protein Pathways: Jak-STAT signaling pathway
• Gene Summary: 'The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]'
  Transcript Variant: This variant (2) lacks a segment in the 5' UTR and coding region compared to variant 1. The translation begins at an in-frame upstream start codon, and results in an isoform (2) with a different N-terminus, as compared to isoform 1.