TBX5 (NM_181486) Human Untagged Clone

CAT#: SC124969

TBX5 (untagged)-Human T-box 5 (TBX5), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX5
• Synonyms: HOS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_181486, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCCTGACGCAAAAGACCTGCCCT
  GCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCGTCCCCGCAGGCCGCCTTCAC
  CCAGCAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTG
  GGCACGGAAATGATCATAACCAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCC
  TTAATCCCAAAACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGC
  AGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGAC
  TCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACC
  ACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAAATACCAGCCTAGATTACACATCGTGAA
  AGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTTCCTGAGACTGCG
  TTTATAGCAGTGACTTCCTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCA
  AAGGATTTCGGGGCAGTGATGACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGT
  GGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTCTCGAGCT
  CTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACC
  TCCTGCCTCCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAA
  AGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAGCCCTACATGGAGACATCACCCAGTGAAGAA
  GATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCAC
  AGCGGCAAGCTTGCATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAG
  CTGCAACACGTGGCCAAGCATGCCTTCCTACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGG
  CTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCTGGCATGGCCA
  ACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGT
  CAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTCCCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCT
  CATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCACGGAGTTGGCATGGTGCCAGAGT
  GGAGCGACAATAGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181486 unedited
  NGGTTCACAATTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGAGTTG
  GCTTGTATTTCAGAGAGAGAGAGAGAGGGAGAGAGAGTGAGAGAGACTGACTCTTACCTC
  GAATCCGGGAACTTTAATCCTGAAAGCTGCGCTCAGAAAGGACTTCGACCATTCACTGGG
  CTTCCAACTTTCCCTCCCTGGGGGTGTAAAGGAGGAGCGGGGCACTGAGATTATATGGTT
  GCCGGTGCTCTTGGAGGCTATTTTGTGTTCTTTGGCGCTTGCCAACTGGGAAGTATTTAG
  GGAGAGCAAGCGCACAGCAGAGGAGGTGTGTGTTGGAGGTGGGCAGTCGCCGCGGAGGCT
  CCAGCGGTAGGTGCGCCCTAGTAGGCAGCAGTAGCCGCTATTCTGGGTAAGCAGTAAACC
  CCGCATAAACCCCGGAGCCACCATGCCTGCTCCCCCGCCTCACCGCCGGCTTCCCTGCTA
  GGAGCAGCAGAGGATGTGGTGAATGCACCGGCTTCACCGAACGAGAGCAGAACCTTGCGC
  GGGCACAGGGCCCTGGGCGCACCATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACA
  CGCCTCTGGAGCCTGACGCAAAAGACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCG
  GNGCCCCCAGCAAGTCCCCGTCGTCCCCGCAGGCCGCCTTCACCCAGCAGGGCATGGAGG
  GAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAANATTCCACGAAGTGGGCACGG
  AAATGATCATAAACCAAGCTGGAAGGCNGATGTTTCCCAGTTACAAAGTGAAGGTGACGG
  GCCTTAATCCCAAACGAATACATTCTTCTCATGGACATTGTACCTGCCGACGACACAGAT
  ACAATCGCAGATATAATGGTCTGTGACGGNCAAGCTGAGCCGCCATGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181486
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181486.1, NP_852259.1
• RefSeq Size: 3749 bp
• RefSeq ORF: 1557 bp
• Locus ID: 6910
• Cytogenetics: 12q24.21
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.