Lysozyme (LYZ) (NM_000239) Human Untagged Clone

CAT#: SC125273

LYZ (untagged)-Human lysozyme (LYZ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LYZ
• Synonyms: LYZF1; LZM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000239 edited
  ATGAAGGCTCTCATTGTTCTGGGGCTTGTCCTCCTTTCTGTTACGGTCCAGGGCAAGGTC
  TTTGAAAGGTGTGAGTTGGCCAGAACTCTGAAAAGATTGGGAATGGATGGCTACAGGGGA
  ATCAGCCTAGCAAACTGGATGTGTTTGGCCAAATGGGAGAGTGGTTACAACACACGAGCT
  ACAAACTACAATGCTGGAGACAGAAGCACTGATTATGGGATATTTCAGATCAATAGCCGC
  TACTGGTGTAATGATGGCAAAACCCCAGGAGCAGTTAATGCCTGTCATTTATCCTGCAGT
  GCTTTGCTGCAAGATAACATCGCTGATGCTGTAGCTTGTGCAAAGAGGGTTGTCCGTGAT
  CCACAAGGCATTAGAGCATGGGTGGCATGGAGAAATCGTTGTCAAAACAGAGATGTCCGT
  CAGTATGTTCAAGGTTGTGGAGTGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000239 unedited
  CCCGTTCAAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAAACCT
  AGCAGTCAACATGAAGGCTCTCATTGTTCTGGGGCTTGTCCTCCTTTCTGTTACGGTCCA
  GGGCAAGGTCTTTGAAAGGTGTGAGTTGGCCAGAACTCTGAAAAGATTGGGAATGGATGG
  CTACAGGGGAATCAGCCTAGCAAACTGGATGTGTTTGGCCAAATGGGAGAGTGGTTACAA
  CACACGAGCTACAAACTACAATGCTGGAGACAGAAGCACTGATTATGGGATATTTCAGAT
  CAATAGCCGCTACTGGTGTAATGATGGCAAAACCCCAGGAGCAGTTAATGCCTGTCATTT
  ATCCTGCAGTGCTTTGCTGCAAGATAACATCGCTGATGCTGTAGCTTGTGCAAAGAGGGT
  TGTCCGTGATCCACAAGGCATTAGAGCATGGGTGGCATGGAGAAATCGTTGTCAAAACAG
  AGATGTCCGTCAGTATGTTCAAGGTTGTGGAGTGTAACTCCAGAATTTTCCTTCTTCAGC
  TCATTTTGTCTCTCTCACATTAAGGGAGTAGGAATTAAGTGAAAGGTCACACTACCATTA
  TTTCCCCTTCAAACAAATAATATTTTTACAGAAGCAGGAGCAAAATATGGCCTTTCTTCT
  AAGAGATATAATGTTCACTAATGTGGTTATTTTATATTAAGCCTACAACATTNTTCAGTT
  TGCANATAGAACTAATACTGGTGAAAATTTACCTAAAACCTTGGTTATCAAATACATCTC
  CAGTACATTCCGTTCTTTTTTTTTTTGAGACAGTCTCGCTCTGTCGCCCCAGCTGGAGTG
  CAGTGGCGCAATCTCGGCTCACTGCAACCTNCACCTCCCGGGTTCACGCCATTCTNCTGC
  CTCACCTNCCGAGTAGCTGGGATTACCGGCGCCCGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000239
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000239.1, NP_000230.1
• RefSeq Size: 1487 bp
• RefSeq ORF: 447 bp
• Locus ID: 4069
• Cytogenetics: 12q15
• Domains: lys
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]'