ARG2 (NM_001172) Human Untagged Clone

CAT#: SC125302

ARG2 (untagged)-Human arginase, type II (ARG2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARG2
• Synonyms: arginase, type II; arginase 2; kidney arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125302 sequence for NM_001172 edited (data generated by NextGen Sequencing)
  ATGTCCCTAAGGGGCAGCCTCTCGCGTCTCCTCCAGACGCGAGTGCATTCCATCCTGAAG
  AAATCCGTCCACTCCGTGGCTGTGATAGGAGCCCCGTTCTCACAAGGGCAGAAAAGAAAA
  GGAGTGGAGCATGGTCCCGCTGCCATAAGAGAAGCTGGCTTGATGAAAAGGCTCTCCAGT
  TTGGGCTGCCACCTAAAAGACTTTGGAGATTTGAGTTTTACTCCAGTCCCCAAAGATGAT
  CTCTACAACAACCTGATAGTGAATCCACGCTCAGTGGGTCTTGCCAACCAGGAACTGGCT
  GAGGTGGTTAGCAGAGCTGTGTCAGATGGCTACAGCTGTGTCACACTGGGAGGAGACCAC
  AGCCTGGCAATCGGTACCATTAGTGGCCATGCCCGACACTGCCCAGACCTTTGTGTTGTC
  TGGGTTGATGCCCATGCTGACATCAACACACCCCTTACCACTTCATCAGGAAATCTCCAT
  GGACAGCCAGTTTCATTTCTCCTCAGAGAACTACAGGATAAGGTACCACAACTCCCAGGA
  TTTTCCTGGATCAAACCTTGTATCTCTTCTGCAAGTATTGTGTATATTGGTCTGAGAGAC
  GTGGACCCTCCTGAACATTTTATTTTAAAGAACTATGATATCCAGTATTTTTCCATGAGA
  GATATTGATCGACTTGGTATCCAGAAGGTCATGGAACGAACATTTGATCTGCTGATTGGC
  AAGAGACAAAGACCAATCCATTTGAGTTTTGATATTGATGCATTTGACCCTACACTGGCT
  CCAGCCACAGGAACTCCTGTTGTCGGGGGACTAACCTATCGAGAAGGCATGTATATTGCT
  GAGGAAATACACAATACAGGGTTGCTATCAGCACTGGATCTTGTTGAAGTCAATCCTCAG
  TTGGCCACCTCAGAGGAAGAGGCGAAGACTACAGCTAACCTGGCAGTAGATGTGATTGCT
  TCAAGCTTTGGTCAGACAAGAGAAGGAGGGCATATTGTCTATGACCAACTTCCTACTCCC
  AGTTCACCAGATGAATCAGAAAATCAAGCACGTGTGAGAATTTAG
  
  Clone variation with respect to NM_001172.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001172 unedited
  GAGGTTCAGCATTTGTNATACGACTCACTATAGGGCGGCCGCNGATTCCCGGGCTCACGG
  GGCGGGCGGACGCTGGCGCGGGTAGGTAAGAGCAGCGGCGGGCGGTGGCGCTCACTCCCG
  GCTTCCAACCGCGCGGAGCCTCTGCCTTGGAGATTCTCAGTGCTGCGGATCATGTCCCTA
  AGGGGCAGCCTCTCGCGTCTCCTCCAGACGCGAGTGCATTCCATCCTGAAGAAATCCGTC
  CACTCCGTGGCTGTGATAGGAGCCCCGTTCTCACAAGGGCAGANAAGAAAAGGAGTGGAG
  CATGGTCCCGCTGCCATAAGAGAAGCTGGCTTGATGAAAAGGCTCTCCAGTTTGGGCTGC
  CACCTAAAAGACTTTGGAGATTTGAGTTTTACTCCAGTCCCCAAAGATGATCTCTACAAC
  AACCTGATAGTGAATCCACGCTCAGTGGGTCTTGCCAACCAGGAACTGGCTGAGGTGGTT
  AGCAGAGCTGTGTCAGATGGCTACAGCTGTGTCACACTGGGAGGAGACCACAGCCTGGCA
  ATCGGTACCATTAGTGGCCATGCCCGACACTGCCCAGACCTTTGTGTTGTCTGGGTTGAT
  GCCCATGCTGACATCAACACACCCCCTTACCACGGCATCAGGAAATCTCCATGGACAGCC
  AGTTTCATTTCTCCTCAGAGAACTACAGGGTAAGGTACCACAACTCCCAGGATTTTCCTG
  GATCAAACCCTGTATCTCTTCTGCAAGTATTGTGTATATTGGTCTGAAAGACGTGGACCC
  TCCTGGAACTTTTATTTTAAAGAACTTGAATCCCGTATTTTTCCTGAAAGATATTGATCG
  ACTGGGTTTCCAAAGGTCCATGGGACGAAATTTGATCTGCTGGTTGCCAAGAAAAAAAAA
  CCAATCCCTTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001172 unedited
  AATTGCATTTTAGCTTTGGTTTTTATGTCTCATTTAACATTTATGTGAGGTAGACAGTGT
  TATCCCTAAGTGACAGCTAAAGAACCTTATGAGTACTTCCTGCTAAGTGGCTGTGTGATC
  AAACATACAGCCTCAGAGTATCCAGCCGTGGTCCCAGGTCTAACCCCAAAATGTTTAGAA
  GGAAGGTTATCCAAACTTCTGACTACTCCCCACTTTGCTTCTCTTTGTAGAGTGACAACC
  AACAAGCTGCTGCTTTCCATTATCATCTTACTGGAGCTCGCCATCCTGGGAGGCCTGGTT
  TACTACAAATTCTTTCGCAGCCATTGAACTTCTATAGGGAAGGGTTTGTGGACCAGAACT
  TTGACACTTGTGAATGCATGATGTTAGGGATGTGGATAGAATAAGCATATTGCTGCTGTG
  GGCTGACAGTTCAAGGATGCACTGTCTAGCCAGGCTGTGGGAGGAGGGAGGAAAGATGAA
  AAACCACTTATATGTGAATGAACAACAGCAACTAGACCAGTATGATATACCAAGGTAATA
  AATGCTGTTTATGACTTCTTTAATGTACATAGTACTGTTACATATTAAATACCCTGGGGA
  CCGCCAAAAACCACATACCTTTACAGTAGTTCTTGGTCACCCAAATAGAGGGGAAACTTT
  ACAATTGTAAGAATGCGGAAATGTTCTTATTAAAGCAGGATTTGCCCCAGAAAACAATTA
  ATTTTTGATTTATCCCCTCAAGGCCTCTAAATTATGGAAAGCCTGTTGTGGAACAAGTTC
  ACGGCAAAGTGGTCTCCTAAATTGCCCACCGTGCTTGGATTTTCTGATCATTTTGGGAAC
  CGGGTATAGGAAGGTGGGAATAACAAAATACCCATCTTTTTTGTTTATGATGAACTTGGA
  GCAATCCTTCGACCGCGCCAGTAAAGCTGATCGTATN
  
• Restriction Sites: Please inquire
• ACCN: NM_001172
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001172.3.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172.3, NP_001163.1
• RefSeq Size: 1981 bp
• RefSeq ORF: 1065 bp
• Locus ID: 384
• Cytogenetics: 14q24.1
• Domains: arginase
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: ' Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]'