CHI3L1 (NM_001276) Human Untagged Clone

CAT#: SC125328

CHI3L1 (untagged)-Human chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHI3L1
• Synonyms: ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; hCGP-39; YK-40; YKL-40; YKL40; YYL-40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125328 sequence for NM_001276 edited (data generated by NextGen Sequencing)
  ATGGGTGTGAAGGCGTCTCAAACAGGCTTTGTGGTCCTGGTGCTGCTCCAGTGCTGCTCT
  GCATACAAACTGGTCTGCTACTACACCAGCTGGTCCCAGTACCGGGAAGGCGATGGGAGC
  TGCTTCCCAGATGCCCTTGACCGCTTCCTCTGTACCCACATCATCTACAGCTTTGCCAAT
  ATAAGCAACGATCACATCGACACCTGGGAGTGGAATGATGTGACGCTCTACGGCATGCTC
  AACACACTCAAGAACAGGAACCCCAACCTGAAGACTCTCTTGTCTGTCGGAGGATGGAAC
  TTTGGGTCTCAAAGATTTTCCAAGATAGCCTCCAACACCCAGAGTCGCCGGACTTTCATC
  AAGTCAGTACCGCTATTTCTGCGCACCCATGGCTTTGATGGGCTGGACCTTGCCTGGCTC
  TACCCTGGACGGAGAGACAAACAGCATTTTACCACCCTAATCAAGGAAATGAAGGCCGAA
  TTTATAAAGGAAGCCCAGCCAGGGAAAAAGCAGCTCCTGCTCAGCGCAGCACTGTCTGCG
  GGGAAGGTCACCATTGACAGCAGCTATGACATTGCCAAGATATCCCAACACCTGGATTTC
  ATTAGCATCATGACCTACGATTTTCATGGAGCCTGGCGTGGGACCACAGGCCATCACAGT
  CCCCTGTTCCGAGGTCAGGAGGATGCAAGTCCTGACAGATTCAGCAACACTGACTATGCT
  GTGGGGTACATGTTGAGGCTGGGGGCTCCTGCCAGTAAGCTGGTGATGGGCATCCCCACC
  TTCGGGAGGAGCTTCACTCTGGCTTCTTCTGAGACTGGTGTTGGAGCCCCAATCTCAGGA
  CCGGGAATTCCAGGCCGGTTCACCAAGGAGGCAGGGACCCTTGCCTACTATGAGATCTGT
  GACTTCCTCCGCGGAGCCACAGTCCATAGAATCCTCGGCCAGCAGGTCCCCTATGCCACC
  AAGGGCAACCAGTGGGTAGGATACGACGACCAGGAAAGCGTCAAAAGCAAGGTGCAGTAC
  CTGAAGGACAGGCAGCTGGCGGGCGCCATGGTATGGGCCCTGGACCTGGATGACTTCCAG
  GGCTCCTTCTGCGGCCAGGATCTGCGCTTCCCTCTCACCAATGCCATCAAGGATGCACTC
  GCTGCAACGTAG
  
  Clone variation with respect to NM_001276.2
  374 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001276 unedited
  TTATAATACGGTGATCTATAGTTGGCCGCGAATTCGGCACGAGGGCCTCGTGCTTTAATT
  CGGCACGAGGGGTATATAAAGGAAGTACAGGGCCTGGGGAAGAGGCCCTGTCTAGGTAGC
  TGGCACCAGGAGCCGTGGGCAAGGGAAGAGGCCACACCCTGCCCTGCTCTGCTGCAGCCA
  GAATGGGTGTGAAGGCGTCTCAAACAGGCTTTGTGGTCCTGGTGCTGCTCCAGTGCTGCT
  CTGCATACAAACTGGTCTGCTACTACACCAGCTGGTCCCAGTACCGGGAAGGCGATGGGA
  GCTGCTTCCCAGATGCCCTTGACCGCTTCCTCTGTACCCACATCATCTACAGCTTTGCCA
  ATATAAGCAACGATCACATCGACACCTGGGAGTGGAATGATGTGACGCTCTACGGCATGC
  TCAACACACTCAAGAACAGGAACCCCAACCTGAAGACTCTCTTGTCTGTCGGAGGATGGA
  ACTTTGGGTCTCAAAGATTTTCCAAGATAGCCTCCAACACCCAGAGTCGCCGGACTTTCA
  TCAAGTCAGTACCGCTATTTCTGCGCACCCATGGCTTTGATGGGCTGGACCTTGCCTGGC
  TCTACCCTGGACGGAGAGACAAACAGCATTTTACCACCCTAATCAAGGAAATGAAGGCCG
  AATTTATAAAGGAAGCCCAGCCAGGGAAAAAGCAGCTCCTGCTCAGCGCAGCCCTGTCTG
  CGGGGAAGGTCACCATTGACAGCAGCTATGACATTGCCAAGAATCCCACACCTGNATTTC
  ATTGCATCATGACTACGATTTTATGGAGCCTGGGTGGGACCACAGCCATACAGTCCCCTG
  TTCGAGGTCAGAGATGCAAGCCTGACGATCAGCACACTGACTTGCTTGGGGTACAGTTAG
  CTGGGGCTCTGCCATAGCTGGGATGGCTCCCCCTTCGAAGAACTCCTTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001276 unedited
  AACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCCCTTGTACTTTATTGACCACCTGGGG
  AATTAGGAAGGGGAAGTAGGATAGGGGACACAATTACCTTCCTAAGGGAAGGTTTCAAGC
  TGGGCTTTGCAGGGGGTATAATTAATATGGCCACTGGGTCTTGTAGGATGTTTGGCTCCT
  TGGGGAAAAAACTTGCCAAAATGGGGCCCTTTTATAAAGAGGGCTGGGGGGCAGGGATTT
  AAAAAAATTCCCTTGCCAGGCTTGGGGATCTGTAAACTTTTCCATTAATCAACAAGGGTG
  TACTAATCCCGAGTTTTACTTTGGGAAGCCTCACTTTCCCCAAAGCCCCATCCCTACCTT
  TTTGCCCACCAGGGTTGAGCTTAAATCTTGGTGTTGGGGACCCTTGCTTAGGCCCCAAGG
  GAGGGAAACTGAGGCTCACCCTGGGACTCAACAGGGCAGGTGATCCGGCTCCCGCCCCCT
  TGTACCCAAAGGGGGACGGGGCTTTCTTGGCCCCCGTTCTTTGTGCAAAACAAAGGGCTA
  CCTTGCAACGAGGGCTTCCTCTTTGGCTTTGCGGAAAGGAAAACCCAAATCCTGTCCCCA
  AAAAGGAACCTGGAAATTTTCCAGATCCCAGGGCCCATACCATGGGGCCCCCCAACTTGC
  CTTGCCTTTAAGTACTGCCCCTTGTTTTGAACTTTTTCTGGCCGCAATTCCCCACCACAT
  TGTTCCCTTTTGGGGCATTGGGCACTCGTTGCCCGAAATCTTAGGACTGGGGCCCCCGGA
  AGAACACCCACCTTATAATCCAGGCGCCCTCCTCTTGGAAACCCCTGTAATCCCGCCTTG
  ATGGGCCCCCCCCCCCCCAAAAACCAAAAAACCCCTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001276
• Insert Size: 1850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276.1, NP_001267.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 1152 bp
• Locus ID: 1116
• Cytogenetics: 1q32.1
• Domains: Glyco_18
• Protein Families: Secreted Protein
• Gene Summary: 'Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]'