AMPK beta 1 (PRKAB1) (NM_006253) Human Untagged Clone

CAT#: SC125443

PRKAB1 (untagged)-Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKAB1
• Synonyms: AMPK; HAMPKb
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006253, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAATACCAGCAGTGAGCGCGCCGCGCTGGAGCGGCATGGTGGCCATAAGACGCCCCGGAGGGACA
  GCTCGGGGGGCACCAAGGACGGGGACAGGCCCAAGATCCTGATGGACAGCCCCGAAGACGCCGACCTCTT
  CCACTCCGAGGAAATCAAGGCACCAGAGAAGGAGGAATTCCTGGCCTGGCAGCATGATCTGGAAGTGAAT
  GATAAAGCTCCCGCCCAGGCTCGGCCAACGGTGTTTCGATGGACGGGGGGCGGAAAGGAAGTTTACTTAT
  CTGGGTCCTTCAACAACTGGAGTAAACTTCCCCTCACCAGAAGCCACAATAACTTTGTAGCCATCCTGGA
  TCTGCCGGAAGGAGAGCATCAGTACAAGTTCTTTGTGGATGGTCAGTGGACGCACGACCCTTCCGAGCCC
  ATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGTGAAGAAAACTGACTTTGAAGTATTTG
  ATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGATGTGTCTGAGCTGTCCAGTTCTCCCCCAGGACCCTA
  CCATCAGGAGCCCTACGTCTGCAAACCCGAAGAGCGCTTTCGGGCACCCCCTATTCTCCCCCCACATCTC
  CTCCAGGTCATCCTGAACAAGGACACGGGGATTTCCTGTGATCCAGCTTTGCTTCCTGAGCCCAATCACG
  TCATGCTGAACCACCTATACGCGCTGTCTATCAAGGATGGAGTGATGGTGCTCAGCGCAACCCACCGGTA
  CAAGAAGAAGTACGTCACCACCTTGTTATACAAGCCCATATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006253 unedited
  NNGGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCAGGTA
  AAGCGCGATTGCGAGAGCTCGGCAACCCTGCCGACTCAGCCGGAACCGGCTCCCGGCCCG
  AGGGGCGTGGTGTCCTGGTGCTCCGACTCCTTCCGCAGGCTCCTTGGGACCCGCGGTTCC
  GGGAGTCCCTTGCTCAGGGTCCCTTTCCTGCAGTGAGGCGCCGTCCGCCTTCCCTGTGTC
  CCCGCAGACCCCCATCATGGGCAATACCAGCAGTGAGCGCGCCGCGCTGGAGCGGCATGG
  TGGCCATAAGACGCCCCGGAGGGACAGCTCGGGGGGCACCAAGGACGGGGACAGGCCCAA
  GATCCTGATGGACAGCCCCGAAGACGCCGACCTCTTCCACTCCGAGGAAATCAAGGCACC
  AGAGAAGGAGGAATTCCTGGCCTGGCAGCATGATCTGGAAGTGAATGATAAAGCTCCCGC
  CCAGGCTCGGCCAACGGTGTTTCGATGGACGGGGGGCGGAAAGGAAGTTTACTTATCTGG
  GTCCTTCAACAACTGGAGTAAACTTCCCCTCACCAGAAGCCACAATAACTTTGTAGCCAT
  CCTGGATCTGCCGGAAGGAGAGCATCAGTACAAGTTCTTTGTGGATGGTCAGTGGACGCA
  CGACCCTTCCGAGCCCATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGT
  GAAGAAAACTGACTNTGAAGTATTTGATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGA
  TGTGTCTGAGCTGTCCAGTTCTCCCCCAGACCCTACCATCANGAGCCCTACGTCTGCAAC
  CCCGAAGAGCGCTTTCGGGCACCCNCTATTCTCCCCCACATCTNCTNCAGNTCATTNCTG
  ACAAGGACACNGNGATTTCCTGTGATCCAGCTTTGCTTCCTGAGCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006253 unedited
  NGACCTTTGGGATGGCAATCTCCATTTCCAGNANAGCACTGGGGCAGGGTCACAGGGATG
  CCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT
  TTTTTTTTTTTTTTACAAAGTTTTATTGAAACTTGTCCATCAAGGTGACAGTAATTCACT
  GTTTTGAAAAGTCACAAAAAAGGGTACCCCCAAACTCAGGTATACCAAGTAAATACTGGA
  ACCCAGGGAGCTCACTCCTCCCTCTCCCACCAGGAGCAAAGGGCATAACGGGAGCTTCCG
  CTGACTCACAGCAACCTGGGCTAGTGGGTACATCTGTGGCGACTCCCGGGAAACTGAGCT
  GCGTATAAAGAAGTCTGCTTTGGTCCAAAGGACATCTTCCTGACCCAGACTCAGAAGGCT
  GCTGTCAAGTTCTACATTCCTTTCAGAATAAGCAACAGCAACTGCTGCAGTCTTGCTAAC
  TAGGGGCAGAGGTCCTGAGCAAGGTGCCACGGGACCTTGTAAAATCACGCTGTGTGATTT
  TATAGGGAAGAGGGCAGAGTGCCTCTCAAGTTTTCACGGTTAACACTGTCACCTATTATG
  ACTGGAATAAAAAGCTGGACAACCTGCCAGCTAACTGGTCAGTCCTGAGGATCATCCAGC
  TGAATGGAGAAATCCGGCAGCAGGTTGAAAAGTCTGTTCTGAGAACAGATTATTGCTGAG
  CAGAGTTCATGTGACTTCTAGACGTGGTGACTTAAAAAATGGCCTTAANGCTGCAGAGCC
  AGCCACCTCTGCTTACAAAAAGAGTACTTAGTGCACATGACTGTNAGAAACATTTGTAAA
  ACCTCATCTAGAAATCAGAAAGCTTNCTATTTCTATANAATGACACCTNCCTGGNACCGA
  GAGACATTCTTGTGTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006253
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006253.4, NP_006244.2
• RefSeq Size: 2412 bp
• RefSeq ORF: 813 bp
• Locus ID: 5564
• Cytogenetics: 12q24.23
• Domains: isoamylase_N, AMPKBI
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]'