SPINK1 (NM_003122) Human Untagged Clone

CAT#: SC125664

SPINK1 (untagged)-Human serine peptidase inhibitor, Kazal type 1 (SPINK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPINK1
• Synonyms: PCTT; PSTI; Spink3; TATI; TCP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003122 edited
  CGCAGAACTTCAGCCATGAAGGTAACAGGCATCTTTCTTCTCAGTGCCTTGGCCCTGTTG
  AGTCTATCTGGTAACACTGGAGCTGACTCCCTGGGAAGAGAGGCCAAATGTTACAATGAA
  CTTAATGGATGCACCAAGATATATGACCCTGTCTGTGGGACTGATGGAAATACTTATCCC
  AATGAATGCGTGTTATGTTTTGAAAATCGGAAACGCCAGACTTCTATCCTCATTCAAAAA
  TCTGGGCCTTGCTGAGAACCAAGGTTTTGAAATCCCATCAGGTCACCGCGAGGCCTGACT
  GGCCTTATTGTTGAATAAATGTATCTGAATATCAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003122 unedited
  TCTGATTTGTATACGACTCACTATAGGCGGCCGCGTATTCAGATCTGGTACCGGCTCCGT
  AATTCCCGGGATCGCAGAACTTCAGCCATGAAGGTAACAGGCATCTTTCTTCTCAGTGCC
  TTGGCCCTGTTGAGTCTATCTGGTAACACTGGAGCTGACTCCCTGGGAAGAGAGGCCAAA
  TGTTACAATGAACTTAATGGATGCACCAAGATATATGACCCTGTCTGTGGGACTGATGGA
  AATACTTATCCCAATGAATGCGTGTTATGTTTTGAAAATCGGAAACGCCAGACTTCTATC
  CTCATTCAAAAATCTGGGCCTTGCTGAGAACCAAGGTTTTGAAATCCCATCAGGTCACCG
  CGAGGCCTGACTGGCCTTATTGTTGAATAAATGTATCTGAATATCNNANAAAAAAAAAAA
  AAAAAAAAAAAAAGGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCAT
  CCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACC
  AGCCTTGTCCTAATAAAATTAAATTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATA
  TTATGGGGTGGAGGGGGGTGGTATTGAGCAAGGGGGCAAGTTGGGAAAACAACCCTGTAG
  GCCTGGGGGGTCTATTGGGACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAA
  TCCTCCCCTCTGGGGTCAAGCGATTTTCCTGGCCTAACCTCCCGAGTTGTTGGGATTCCC
  AGCCTGCATGACCAGGCTAAATATATTTTGTTTTTTTGTAAAAACGGGGTTCCCCTTTTG
  CCAGGGGGGGTCCAACCCTAAACCGGGAGTCACCCCCCTTGGCCCCCAATTGGGGGTAAC
  AGGGGGAACAAGGGCCTTCCGGGCCTCTTTATAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003122
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003122.2, NP_003113.2
• RefSeq Size: 438 bp
• RefSeq ORF: 240 bp
• Locus ID: 6690
• Cytogenetics: 5q32
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.