T box 2 (TBX2) (NM_005994) Human Untagged Clone

CAT#: SC125962

TBX2 (untagged)-Human T-box 2 (TBX2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX2
• Synonyms: VETD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125962 sequence for NM_005994 edited (data generated by NextGen Sequencing)
  
  
  ATGAGAGAGCCGGCGCTGGCGGCCAGCGCCATGGCTTACCACCCGTTCCACGCGCCACGGCCCGCCGACT
  TCCCCATGTCCGCCTTTCTGGCGGCGGCGCAGCCCTCCTTCTTCCCGGCACTCGCGCTGCCGCCCGGCGC
  GCTGGCCAAGCCGCTGCCCGACCCGGGCCTGGCGGGGGCGGCGGCCGCGGCGGCGGCGGCGGCAGCAGCG
  GCCGAGGCGGGGCTGCACGTCTCGGCACTGGGCCCGCACCCGCCCGCCGCGCATCTGCGCTCCCTCAAGA
  GCCTGGAGCCCGAGGACGAGGTGGAGGACGACCCCAAGGTGACGCTGGAGGCCAAGGAGCTGTGGGACCA
  GTTCCACAAGCTAGGCACGGAGATGGTCATCACCAAGTCCGGGAGGCGGATGTTCCCCCCCTTCAAGGTG
  CGAGTCAGCGGCCTGGACAAGAAGGCCAAGTATATCCTGCTGATGGACATTGTAGCCGCTGACGATTGCC
  GCTATAAGTTCCACAACTCGCGCTGGATGGTGGCGGGCAAGGCCGACCCTGAGATGCCCAAACGCATGTA
  CATCCACCCAGACAGCCCAGCCACGGGGGAGCAGTGGATGGCTAAGCCTGTGGCCTTCCACAAGCTGAAG
  CTGACCAACAACATCTCTGACAAGCACGGCTTCACCATCCTAAACTCCATGCACAAGTACCAGCCGCGCT
  TCCACATAGTGCGAGCCAACGACATCCTGAAGCTGCCTTACAGCACCTTCCGCACCTACGTGTTCCCGGA
  GACCGACTTCATCGCCGTCACTGCCTACCAGAATGACAAGATCACACAGCTGAAGATCGACAACAACCCG
  TTTGCCAAGGGCTTCCGGGACACCGGGAACGGCCGGCGGGAGAAAAGGAAGCAGCTGACGCTGCCGTCTC
  TACGCTTGTACGAGGAGCACTGCAAACCCGAGCGCGATGGCGCGGAGTCAGACGCCTCGTCGTGCGACCC
  TCCCCCCGCGCGGGAACCACCCACCTCCCCGGGCGCAGCGCCCAGTCCGCTGCGCCTGCACCGGGCCCGA
  GCTGAGGAGAAGTCGTGCGCCGCGGACAGCGACCCGGAGCCTGAGCGGTTGAGCGAGGAGCGTGCGGGGG
  CGCCGCTAGGCCGCAGCCCGGCTCCAGACAGCGCCAGCCCCACTCGCTTGACCGAACCCGAGCGCGCCCG
  GGAGCGGCGTAGTCCCGAGAGGGGCAAGGAGCCGGCCGAGAGCGGCGGGGACGGCCCGTTCGGCCTGAGG
  AGCCTGGAGAAGGAGCGCGCCGAAGCTCGGAGGAAGGACGAGGGGCGCAAGGAGGCGGCCGAGGGCAAGG
  AGCAGGGCCTGGCGCCGCTGGTGGTGCAGACAGACAGTGCGTCCCCCCTGGGCGCCGGACACCTGCCCGG
  CCTGGCCTTTTCCAGCCACTTGCACGGGCAGCAGTTCTTTGGGCCGCTGGGAGCCGGCCAGCCGCTCTTC
  CTGCACCCTGGACAGTTCACCATGGGCCCTGGCGCCTTCTCCGCCATGGGCATGGGTCACCTACTGGCCT
  CGGTGGCAGGCGGCGGCAACGGCGGAGGTGGCGGGCCTGGGACCGCCGCGGGGCTGGACGCAGGCGGGCT
  GGGTCCCGCGGCCAGCGCAGCAAGCACCGCCGCGCCCTTCCCGTTCCACCTCTCCCAGCACATGCTGGCA
  TCTCAGGGAATTCCAATGCCCACTTTCGGAGGCCTCTTCCCCTACCCCTACACCTACATGGCAGCAGCAG
  CCGCAGCCGCCTCGGCTTTGCCCGCCACTAGTGCTGCAGCTGCCGCCGCCGCAGCCGCCGGCTCCCTCTC
  CCGGAGTCCCTTCCTGGGCAGTGCCCGGCCCCGACTGCGTTTCAGCCCCTATCAGATCCCGGTCACCATC
  CCGCCTAGCACTAGCCTCCTCACCACCGGGCTGGCCTCTGAGGGCTCCAAGGCCGCTGGTGGAAACAGCC
  GGGAGCCTAGCCCCCTGCCCGAGCTGGCTCTCCGCAAAGTAGGGGCCCCATCCCGCGGTGCCCTGTCGCC
  CAGTGGCTCGGCCAAGGAGGCGGCCAATGAACTGCAGAGCATCCAGAGACTGGTGAGTGGGCTGGAGAGC
  CAGCGAGCCCTCTCCCCAGGCCGGGAGTCGCCCAAGTGA
  
  Clone variation with respect to NM_005994.3
  1827:t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005994 unedited
  GGGGTTGCCATTTGTATACGACTTACTTNGGGCGGCCGCGCAATTCGCACGAGGCAGAGA
  TCACGACAAGATCTAACCAGTCGCGCGTGGTCCCCGGCGCCGGAGCGGGCCAGCTCAGCC
  CGGCCCAGCCCGGCCCCGCGCAGAGCCCCCGCCGCCCCCGCGCACAGAGCCGGGTGCCCC
  TTGCGGTGCGCCGGACGGGAAGCCCCGAGGAGCAGCTGCTGCGCCCGCCACCCGGGTCGT
  CCGTCCACCGCGCGCGCCGCCGCCCGGGCCGGGGGTCCGAGCCGCGCGCCCCCGGCCCCG
  GCCCCGGCCCCCGGGCGCCTGGGCCGGATGTCCCGATGAGAGAGCCGGCGCTGGCGGCCA
  GCGCCATGGCTTACCACCCGTTCCACGCGCCACGGCCCGCCGACTTCCCCATGTCCGCCT
  TTCTGGCGGCGGCGCAGCCCTCCTTCTTCCCGGCACTCGCGCTGCCGCCCGGCGCGCTGG
  CCAAGCCGCTGCCCGACCCGGGCCTGGCGGGGGCGGCGGCCGCGGCGGCGGCGGCGGCAG
  CAGCGGCCGAGGCGGGGCTGCACGTCTCGGCACTGGGCCCGCACCCGCCCGCCGCGCATC
  TGCGCTCCCTCAAGAGCCTGGAGCCCGAGGACGAGGTGGAGGACGACCCCAAGGTGACGC
  TGGAGGCCAAGGAGCTGTGGGACCAGTTCCACAAGCTAGGCACGGAGATGGTCATCACCA
  AGTCCGGGAGGCGGATGTTCCCCCCCTTCAAGTGCGAGTCAGCGGCCTGGGACAGAGGCC
  AAGTATATCCTGCTGATGGCATTGTAGCCGCTGACGATTGCCGCTTTAGTTTCACAACTC
  GCGCTGGATGTGGCGGGCAATGCCGACCTGAGATGCCCAACGCTGTTCATCCACCAGACG
  CCCACCACGGGGGACA
  
• Restriction Sites: Please inquire
• ACCN: NM_005994
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005994.3, NP_005985.3
• RefSeq Size: 3396 bp
• RefSeq ORF: 2139 bp
• Locus ID: 6909
• Cytogenetics: 17q23.2
• Protein Families: Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product is the human homolog of mouse Tbx2, and shares strong sequence similarity with Drosophila omb protein. Expression studies indicate that this gene may have a potential role in tumorigenesis as an immortalizing agent. Transcript heterogeneity due to alternative polyadenylation has been noted for this gene. [provided by RefSeq, Jul 2008]'