PRAS40 (AKT1S1) (NM_032375) Human Untagged Clone

CAT#: SC126007

AKT1S1 (untagged)-Human AKT1 substrate 1 (proline-rich) (AKT1S1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKT1S1
• Synonyms: Lobe; PRAS40
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032375 edited
  GACAGCTGGAAGAGCCGAACGGATAAGAGAAGAGGAGGTGAGAGGAGCTGTACACCACAA
  GAGGCACTGAGGGACTCAGGATAACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAAGCG
  GCCCCGGACGAATTTCTGAGTCACCGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAG
  CCTGGCAAACCGAAGCAAGAAATGCTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAA
  TGGGAGCAGGTTGGAGGGAGGGAGAGCCAATATACACTATGGGCTGATTAAGCCCGGTTG
  GCTGCCATGTTGTTAACGAGCACCGATTTCCTCTACTTTTGTCGAAGAAGTTTATTGTGG
  GTCAGGGACGTCAGGTCGCTTGCCTTCGTTTACTGTGGTCATGATTGAGCATATGAGGAC
  GGCCATTATTGTTGGGGGCAAATGGAAATGCTCTAGGCGGGGCCATTTTTCTTAGGGGCA
  AGCTGTCGTCACCCTTGTCAACTGGTTCGGATGAAGCCCCTGTGGCCGCCATCTTGATCT
  CGGGCGGCCCCGATAAGGGAGGCGGAGTGTGCGGAGAGGAGGCGGGGCAACTGCGCGGAC
  GTGACGCAAGGCGCCGCCATGTCTTTTGAGGGCGGTGACGGCGCCGGGCCGGCCATGCTG
  GCTACGGGCACGGCGCGGATGGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTG
  GGGGCCGCTGAGCGCTTCCGGGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCC
  CCGCCGCCACCACCCCGCCCGGGCCCCTGTGCCTATCCTGCCCATGGTCGAGGAGCCCTG
  GCGGAGGCAGCGCGCCGTTGCCTCCACGACATCGCACTGGCCCACAGGGCTGCCACTGCT
  GCTCGGCCTCCTGCGCCCCCACCAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCC
  CGGCCTACCCTGGCCAGAGAGGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAG
  ACCTCCGGGGAGCAGCTGGGCATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGAC
  GCCACCCTCCAGGACCTTCCCCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGGC
  AGCCTGAGCGAGGAGACCCCCGCCGGCCCCCCCACCTGCTCAGTGCCCCCAGCCTCAGCC
  CTACCCACACAGCAGTACGCCAAGTCCCTGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAG
  GAGAAGAGGACAGAGGCGCGGTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGAC
  CTGGACCGCATCGCGGCGAGCATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAG
  GTCTTCGGGGACCTGCCACGGCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCGG
  AAATATTGAAGTCCAGGGAGGGAGCGCCCCGGGCCGCGTCCGCCCCGTCCCACACTACGC
  CCCCGCCCCACTCCCGGGGCCTGCTAATCTGAGGCCGATCCGGGACCGGCCTCCTTGCGT
  CTCCCATTCCCAAGATTGTCCCGCCTCTGCCAATCCCCGCCGTCCTTCCAGCCCACGACC
  TGCCGCGCCGAGGAGCGGCATCTGTCCCGTTTCCCGATTGGGTCTGTCGTCTCTCTCCGC
  CTAGCGACAGATTCCTTCTATTAAGGGATTGGCTCGCTGAGTTCTAAGCTCTAAATGGGT
  CAACTCCTTTGTTTTCCGCCTAGCGACAAGGGATTTGCTCGCACGGCATTGGCTCCATCC
  CCTAGTCGCTGGACAGCTCTTTTTTTGATTGGCTCAAATCCTGTAAAGGGCTTGACCAGT
  CTCTACATAGTCACCGTCCGCTTTTCCTGAGTTCTCCCTCCCAATTGGCTCCAGCTTCCT
  GGGGGCGTGGCCAAGCCCTCCTCTTCCCAGAATTGGCCCGGGGCCTTCAATTTACGTTCT
  TTACACTACGGGGACTGGGGTCGTCTTTGCCCACGTCCCGACAACTTGTTCCCTGACCCC
  CTCAGGGATGGCCCCAAACTGTCCCTGCCTCTGGCACCCCCTTTCATTGGTTCCATCCAT
  CCCCACAACAGCCTGCCAATCGAAGCCCGTCCCTGCATCCAGGATGGTACCAGCTCCCGC
  CCCTCGCCCCCCACCTCCACAGGTGCCTTAAAGGGCCCTCGTCCACCCAAGGTGGGGGGC
  AGGGGCCCTCACTCTCCGGCCCTGGTGTGGGGGAGAGAGTGAGGGGTTGGGGGATCGGCA
  GTTGGGAGGGGCGCTCTGAGATTAAAGAGTTTTACCTCTGAGATAAAAAAAAAAAAAAAA
  AAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032375 unedited
  GGTTCGGATTTGTATACGACTTATATAGGGCGGCCGCGAATTCGCACGAGGGACAGCTGG
  AAGAGCCGAACGGATAAGAGAAGAGGAGGTGAGAGGAGCTGTACACCACAAGAGGCACTG
  AGGGACTCAGGATAACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAAGCGGCCCCGGAC
  GAATTTCTGAGTCACCGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAGCCTGGCAAA
  CCGAAGCAAGAAATGCTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAATGGGAGCAG
  GTTGGAGGGAGGGAGAGCCAATATACACTATGGGCTGATTAAGCCCGGTTGGCTGCCATG
  TTGTTAACGAGCACCGATTTCCTCTACTTTTGTCGAAGAAGTTTATTGTGGGTCAGGGAC
  GTCAGGTCGCTTGCCTTCGTTTACTGTGGTCATGATTGAGCATATGAGGACGGCCATTAT
  TGTTGGGGGCAAATGGAAATGCTCTAGGCGGGGCCATTTTTCTTAGGGGCAAGCTGTCGT
  CACCCTTGTCAACTGGTTCGGATGAAGCCCCTGTGGCCGCCATCTTGATCTCGGGCGGCC
  CCGATAAGGGAGGCGGAGTGTGCGGAGAGGAGGCGGGGCAACTGCGCGGACGTGACGCAA
  GGCGCCGCCATGTCTTTTGAGGGCGGTGACGGCGCCGGGGCCGCCATGCTGGCTACGGGC
  ACGGCGCGGATGGCGTCNGGGCGCCCCGAGGAGCTGTGGAAGCCCTGGTTTGNGGNCNNT
  TANNNNTTTTCGGNNCCGNCTGGCCCGGGCTTGTGCTGCTTGCCGGGGCCCCGCGCCCCC
  TCCGGGCCGGTCCGGGTCCTTTCCTTGCCTTGTCCNGGGCCCCGGGGGTGGCCCCGCCGT
  TGCTCCCCGCCTGTCCGGTCGCTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_032375
• ORF Size: 771 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_032375.2, NP_115751.1
• RefSeq Size: 2344
• RefSeq ORF: 771
• Locus ID: 84335
• Gene Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.