GFI1 (NM_005263) Human Untagged Clone

CAT#: SC126131

GFI1 (untagged)-Human growth factor independent 1 transcription repressor (GFI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFI1
• Synonyms: GFI-1; GFI1A; SCN2; ZNF163
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005263 edited
  CCACGCGTCCGGGTGCCCGCGCCGTGACTCCGCAGGTCTCCTCCGGGCTGGGGCTGAGCA
  AGCCCTCGGAGTGACCGTGGGTGACAGCGGCTCCAGGGACTCTTGGGGCGCAGTGGGGAA
  AGTGCCGGACCACCATGCCGCGCTCATTTCTCGTCAAAAGCAAGAAGGCTCACAGCTACC
  ACCAGCCGCGCTCCCCAGGACCAGACTATTCCCTCCGTTTAGAGAATGTACCGGCGCCTA
  GCCGAGCAGACAGCACTTCAAATGCAGGCGGGGCGAAGGCGGAGCCCCGGGACCGTTTGT
  CCCCCGAATCGCAGCTGACCGAAGCCCCAGACAGAGCCTCCGCATCCCCAGACAGCTGCG
  AAGGCAGCGTCTGCGAACGGAGCTCGGAGTTTGAGGACTTCTGGAGGCCCCCGTCACCCT
  CCGCGTCTCCAGCCTCGGAGAAGTCAATGTGCCCATCGCTGGACGAAGCCCAGCCCTTCC
  CCCTGCCTTTCAAACCGTACTCATGGAGCGGCCTGGCGGGTTCTGACCTGCGGCACCTGG
  TGCAGAGCTACCGACCGTGTGGGGCCCTGGAGCGTGGCGCTGGCCTGGGCCTCTTCTGTG
  AACCCGCCCCGGAGCCTGGCCACCCGGCCGCGCTGTACGGCCCGAAGCGGGCTGCCGGCG
  GCGCGGGGGCCGGGGCGCCAGGGAGCTGCAGCGCAGGGGCCGGTGCCACCGCTGGCCCTG
  GCCTAGGGCTCTACGGCGACTTCGGGTCTGCGGCAGCCGGGCTGTATGAGAGGCCCACGG
  CAGCGGCGGGCTTGCTGTACCCCGAGCGTGGCCACGGGCTGCACGCAGACAAGGGCGCTG
  GCGTCAAGGTGGAGTCGGAGCTGCTGTGCACCCGCCTGCTGCTGGGCGGCGGCTCCTACA
  AGTGCATCAAGTGCAGCAAGGTGTTCTCCACGCCGCACGGGCTCGAGGTGCACGTGCGCA
  GGTCCCACAGCGGTACCAGACCCTTTGCCTGCGAGATGTGCGGCAAGACCTTCGGGCACG
  CGGTGAGCCTGGAGCAGCACAAAGCCGTGCACTCGCAGGAACGGAGCTTTGACTGTAAGA
  TCTGTGGGAAGAGCTTCAAGAGGTCATCCACACTGTCCACACACCTGCTTATCCACTCAG
  ACACTCGGCCCTACCCCTGTCAGTACTGTGGCAAGAGGTTCCACCAGAAGTCAGACATGA
  AGAAACACACTTTCATCCACACTGGTGAGAAGCCTCACAAGTGCCAGGTGTGCGGCAAGG
  CATTCAGCCAGAGCTCCAACCTCATCACCCACAGCCGCAAACACACAGGCTTCAAGCCCT
  TCGGCTGCGACCTCTGTGGGAAGGGTTTCCAGAGGAAGGTGGACCTCCGAAGGCACCGGG
  AGACGCAGCATGGGCTCAAATGAGCACCCTGGCTGGCTGCAAGCAGCAGCTACACAACAC
  TACAGAGGGCAGCCTCCCTGCTTGCCACCACTCTGCTCCCTGCTTGCCTCCACTCCCTTC
  TGACTTTCCAGACCCCAGGTCCAGTCTGCAGATCCTACCAGGTTGCTCCTCCTTCGCCTT
  ACCTCCTGGAGCTGCCAGAAGAAATGAGGTACCTTTTCAAAGTGCAGCCGAGAGTGAGAA
  CCAAGTGACTCCCTAGGCTTCGGACACAAATAGGCTCCTCTACACCTGAAGACAAAGGCA
  AAGTCAAATGGGGACCAGAATAAATCTTAGACCCCACAGTCCTTCCCATTTCCAGCCCTA
  ATCTACAGACAGGAATGCCCTTCAGGTTTCTTCCCTCCCCCCTCTTGACCTACCCCAGAT
  ATTTGTGTGGAAGAGGAGGAATCACCATTTACAAGGTGGACAAATGCTAATATTTTTATC
  TAGAAAGAAGAGTGAGTGTTAACTTTTATTTTTTTCCTTCTGGGGGGTCTGTTGACTCCT
  TTCTTTTGGGTGCTGCCTATAAATCTTGGAGGAATCATTTCTCCTCCTCAAAAACTGATT
  CAGAAACTGACTTGGGGAAGGAATTTAATACTTTGAAGTCATGAGATGCACCATCGAGGC
  TACCCCCAAGAAGAAGCAGAAGAGAAGTTGGTAATGAGAGGGGATTAGAGGTCCTCCCTT
  CAGTAGGGCTGTGAAAACCTCATCACTGGAGGTAAAAGCACAAGCAATGCCTGTGGACAA
  GATGTCATTCATTCACTCAGCAAATGTTCATGGATCACCGGCTACCAAGGTACCAGGCAC
  CATGCTAGGTATTGGGGAAGAGAGACTGAAGTCACAACCCCTGACTGCTCCTCAAAAGCT
  AACGGTTGCACCTCCAAGTGGCTGGGTCTGTTCTTACTCTTGGAGGGAATTCTGAGAAGA
  CAGCACAGAATTGTAAACCTTCCCTTTTGACCCTTTTGGATTTTATCAGGTGTAAACAAA
  AAGCTGAACAGTTACTTCAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005263 unedited
  GGGTTTTTTATTTGTATACGACTTATATAGGCGGCCGCGNAATTCNATCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGTGCCCGCGCCGTGACTCCGCAGG
  TCTCCTCCGGGCTGGGGCTGAGCAAGCCCTCGGAGTGACCGTGGGTGACAGCGGCTCCAG
  GGACTCTTGGGGCGCAGTGGGGAAAGTGCCGGACCACCATGCCGCGCTCATTTCTCGTCA
  AAAGCAAGAAGGCTCACAGCTACCACCAGCCGCGCTCCCCAGGACCAGACTATTCCCTCC
  GTTTAGAGAATGTACCGGCGCCTAGCCGAGCAGACAGCACTTCAAATGCAGGCGGGGCGA
  AGGCGGAGCCCCGGGACCGTTTGTCCCCCGAATCGCAGCTGACCGAAGCCCCAGACAGAG
  CCTCCGCATCCCCAGACAGCTGCGAAGGCAGCGTCTGCGAACGGAGCTCGGAGTTTGAGG
  ACTTCTGGAGGCCCCCGTCACCCTCCGCGTCTCCAGCCTCGGAGAAGTCAATGTGCCCAT
  CGCTGGACGAAGCCCAGCCCTTCCCCCTGCCTTTCAAACCGTACTCATGGAGCGGCCTGG
  CGGGTTCTGACCTGCGGCACCTGGTGCAGAGCTACCGACCGTGTGGGGCCCTGGAGCGTG
  GCGCTGGCCTGGGCCTCTTCTGTGAACCCGCCCCGGAGCCTGGCCACCCGGCCGCGCTGT
  ACGGCCCGAAGCGGGCTGCCGGCGGCGCGGGGGCCGGGGCGCCAGGGAGCTGCAGCGCAG
  GGGCCGGTGCCACCGCTGGCCCTGGCCTAGGGCTCTACNGCGACTTCGGGTCTGCNGCAG
  CCGGGCTGTATGAGAAGCCCACGGCAGCGGCGGGCTTGCTGTACCCCGAGCGTGGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005263
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005263.2, NP_005254.2
• RefSeq Size: 2784 bp
• RefSeq ORF: 1269 bp
• Locus ID: 2672
• Cytogenetics: 1p22.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.