CST6 (NM_001323) Human Untagged Clone

CAT#: SC126202

CST6 (untagged)-Human cystatin E/M (CST6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CST6
• Synonyms: cystatin 6; cystatin E; cystatin M; cysteine proteinase inhibitor; E; M
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001323 edited
  GAGGGCTCCGACGGCACTGACGGCCATGGCGCGTTCGAACCTCCCGCTGGCGCTGGGCCT
  GGCCCTGGTCGCATTCTGCCTCCTGGCGCTGCCACGCGACGCCCGGGCCCGGCCGCAGGA
  GCGCATGGTCGGAGAACTCCGGGACCTGTCGCCCGACGACCCGCAGGTGCAGAAGGCGGC
  GCAGGCGGCCGTGGCCAGCTACAACATGGGCAGCAACAGCATCTACTACTTCCGAGACAC
  GCACATCATCAAGGCGCAGAGCCAGCTGGTGGCCGGCATCAAGTACTTCCTGACGATGGA
  GATGGGGAGCACAGACTGCCGCAAGACCAGGGTCACTGGAGACCACGTCGACCTCACCAC
  TTGCCCCCTGGCAGCAGGGGCGCAGCAGGAGAAGCTGCGCTGTGACTTTGAGGTCCTTGT
  GGTTCCCTGGCAGAACTCCTCTCAGCTCCTAAAGCACAACTGTGTGCAGATGTGATAAGT
  CCCCGAGGGCGAAGGCCATTGGGTTTGGGGCCATGGTGGAGGGCACTTCAGGTCCGTGGG
  CCGTATCTGTCACAATAAATGGCCAGTGCTGCTTCTTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001323 unedited
  GGTTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGAGGGCTC
  CGACGGCACTGACGGCCATGGCGCGTTCGAACCTCCCGCTGGCGCTGGGCCTGGCCCTGG
  TCGCATTCTGCCTCCTGGCGCTGCCACGCGACGCCCGGGCCCGGCCGCAGGAGCGCATGG
  TCGGAGAACTCCGGGACCTGTCGCCCGACGACCCGCAGGTGCAGAAGGCGGCGCATGCGG
  CCGTGGCCAGCTACAACATGGGCAGCAACAGCATCTACTACTTCCGAGACACGCACATCA
  TCAAGGCGCAGAGCCAGCTGGTGGCCGGCATCAAGTACTTCCTGACGATGGAGATGGGGA
  GCACAGACTGCCGCAAGACCAGGGTCACTGGAGACCACGTCGACCTCACCACTTGCCCCC
  TGGCAGCAGGGGCGCATCAGGAGAAGCTGCGCTGTGACTTTGAGGTCCTTGTGGTTCCCT
  GGCAGAACTCCTCTCAGCTCCTAAAGCACAACTGTGTGCAGATGTGATAAGTCCCCGAGG
  GCGAAGGCCATTGGGTTTGGGGCCATGGTGGAGGGCACTTCANGTCCGTGGGCCGTATCT
  GTCACAATAAATGGGCAGTGCTGCTTCTTAAAAAAAAAAAAAAAAAACTCGACTCTAGAT
  TGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTC
  CCCAGTGCCTCTCCTGGGCCCTGGAAGTGCCACTCCAGTGCCCACCAGCCTTGTGCTAAA
  TAAAATAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATATGGGGTGGAGG
  GGGGGGGGTGGGACCAAGGGGCAATTGGAAAACACCTTTAGGGCCGCGGGGCTATGGGGA
  CCAACTTGGGGGAGGGGCCCAACTGGTAATGAGTTTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001323
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001323.2, NP_001314.1
• RefSeq Size: 627 bp
• RefSeq ORF: 450 bp
• Locus ID: 1474
• Cytogenetics: 11q13.1
• Protein Families: Secreted Protein
• Gene Summary: 'The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008]'