CPN1 (BC027897) Human Untagged Clone

CAT#: SC126271

CPN1 (untagged)-Human carboxypeptidase N, polypeptide 1, 50kD, mRNA (cDNA clone MGC:34611 IMAGE:5227795), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPN1
• Synonyms: anaphylatoxin inactivator; arginine carboxypeptidase; carboxypeptidase N, polypeptide 1, 50kD; carboxypeptidase N catalytic subunit; carboxypeptidase N polypeptide 1 50 kD; carboxypeptidase N small subunit; CPN; FLJ40792; kininas; OTTHUMP00000020269; SCPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC027897 edited
  TGCAAAAAACCTTTCAAAAGGCTTTCCTGGATTAGAGAAAGAAAGGGAGTGAGGGAGGAG
  AGATGAGTGGCTATTCCAGAACGACATAAAGAATTTCCAGCCTTGGACGGACAGCTGGGA
  ACGTCTTCCAATTTGGACTGGTGTTTACAAGCGGGAAGCTAGGTGGACCTTGGATTTTGG
  CGGGTGAAGAGGCTAGGTTGTTTAAGGAGGTGGGGCGCGTTTCAGTGGCTCTCTTTGAAA
  AAGCCCAGCAAGATGTCAGACCTGCTCTCAGTCTTCCTCCACCTCCTCCTTCTCTTCAAG
  TTGGTTGCCCCGGTGACCTTTCGCCACCACCGCTATGATGATCTTGTGCGGACGCTGTAC
  AAGGTGCAAAACGAATGCCCCGGCATCACGCGGGTCTACAGCATTGGGCGCAGCGTGGAG
  GGGAGACACCTCTACGTGCTGGAGTTCAGCGACCACCCTGGAATCCACGAGCCCTTGGAA
  CCAGAGGTCAAGTATGTGGGGAACATGCACGGCAACGAAGCGTTGGGCCGCGAGCTGATG
  CTGCAGCTGTCGGAGTTTCTGTGCGAGGAGTTCCGGAACAGGAACCAGCGCATCGTCCAG
  CTCATCCAGGACACGCGCATTCACATCCTGCCATCCATGAACCCCGACGGCTACGAGGTG
  GCTGCTGCCCAGGGCCCAAACAAGCCTGGGTATCTAGTTGGCAGGAACAATGCAAATGGA
  GTGGACCTGAACCGCAACTTCCCTGATCTCAATACCTATATCTACTATAACGAGAAGTAC
  GGAGGCCCCAACCACCACCTGCCCCTTCCAGACAACTGGAAAAGTCAGGTGGAACCCGAG
  ACCCGGGCGGTGATCCGGTGGATGCACTCCTTCAACTTTGTTCTTTCAGCCAATCTCCAC
  GGAGGGGCGGTGGTGGCCAATTACCCGTATGACAAGTCCTTTGAGCACCGGGTCCGAGGG
  GTCCGCCGCACCGCCAGCACCCCCACGCCTGACGACAAGCTCTTCCAGAAGCTGGCCAAG
  GTCTACTCCTATGCACATGGATGGATGTTCCAAGGTTGGAACTGCGGAGATTACTTCCCA
  GATGGCATCACCAATGGAGCTTCCTGGTATTCTCTCAGCAAGGGAATGCAAGACTTTAAT
  TATCTCCATACCAACTGCTTTGAGATCACGCTGGAACTGAGTTGCGACAAGTTTCCCCCC
  GAAGAGGAGTTACAGCGGGAGTGGCTGGGTAATCGGGAAGCCCTAATCCAGTTCCTGGAA
  CAGGTTCACCAGGGCATCAAGGGAATGGTGCTTGATGAGAATTACAATAATCTCGCCAAT
  GCTGTCATTTCTGTCAGTGGGATTAACCATGATGTCACTTCAGGTGACCATGGTGATTAC
  TTCCGGCTGCTGCTTCCAGGTATCTACACTGTTAGTGCCACAGCACCTGGGTATGACCCA
  GAGACAGTAACTGTGACCGTGGGTCCTGCGGAACCAACGTTGGTTAACTTCCACCTCAAA
  AGAAGCATCCCTCAAGTAAGCCCTGTGAGGAGAGCTCCCAGCAGAAGGCACGGAGTCAGA
  GCCAAAGTGCAGCCCCAAGCCAGAAAGAAAGAAATGGAGATGAGGCAGCTGCAGAGAGGC
  CCTGCCTGAAACCCACAGTGCCAGGCAACCCTTCAGAAAGGCTTTGCTCCTGCTCTCAGA
  TCAGATCAAGCATTCTTTCTATTTTATTATCTGGGACATATTTAAATACAAACATATTCA
  GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC027897 unedited
  GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCANATCTGGTACCGGTCCGGA
  ATTCCCGGGATTGCAAAAAACCTTTCAAAAGCTTTCCTGGATTAGAGAAAGAAAGGGAGT
  GAGGGAGGAGAGATGAGTGGCTATTCCAGAACGACATAAAGAATTTCCAGCCTTGGACGG
  ACAGCTGGGAACGTCTTCCAATTTGGACTGGTGTTTACAAGCGGGAAGCTAGGTGGACCT
  TGGATTTTGGCGGGTGAAGAGGCTAGGTTGTTTAAGGAGGTGGGGCGCGTTTCAGTGGCT
  CTCTTTGAAAAAGCCCAGCAAGATGTCAGACCTGCTCTCAGTCTTCCTCCACCTCCTCCT
  TCTCTTCAAGTTGGTTGCCCCGGTGACCTTTCGCCACCACCGCTATGATGATCTTGTGCG
  GACGCTGTACAAGGTGCAAAACGAATGCCCCGGCATCACGCGGGTCTACAGCATTGGGCG
  CAGCGTGGAGGGGAGACACCTCTACGTGCTGGAGTTCAGCGACCACCCTGGAATCCACGA
  GCCCTTGGAACCAGAGGTCAAGTATGTGGNGAACATGCACGGCAACGAAGCGTTGGGCCG
  CGAGCTGATGCTGCAGCTGTCGGAGTTTCTGTGCGAGGAGTTCCGGAACANGAACCAGCG
  CATCGTCCAGCTCATCCAGGACACGCGCATTCACATCCTGCCATCCATGAACCCCCGACG
  CTACAAGGTGGCTGCTGCCCAGGGCCAAAACAGCCTGGGTATCTAGTTGGCAGGGAACAT
  GCAAATGGGAGTGGACCTGAACCCGCACTTCCTGGATCTCATACCTATTTCTACTTTACG
  AGGAGTACGGGAGGCCCCAACAACACCTGGCCCCTTCCGAAACTGGAAAAATCAGGTGGG
  AACCCGAACCCGGGCCGGGGATCCGGGGGAGCCCTCCTTCCACTTTGGTCTTTAACCCAA
  TTCCCCGGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: BC027897
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027897.1, AAH27897.1
• RefSeq Size: 1784 bp
• Locus ID: 1369
• Cytogenetics: 10q24.2
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: 'Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]'