CTBP1 (NM_001012614) Human Untagged Clone

CAT#: SC126399

CTBP1 (untagged)-Human C-terminal binding protein 1 (CTBP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTBP1
• Synonyms: BARS; HADDTS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001012614 edited
  GCGCGGCTCAGCGGCGCGGCGGAGACTCGGCACGGCGGCGGCCAGGCGCAGGCGGGCGGG
  CGGAGCAGCGGACGGCGCCGAGGCCGCCCCACGCGCCGGGCTCGCGGCGGCGCAGCGCGC
  CGGAGTGGTCGGGGCCCGCGGCCGCTCGCGCCTCTCGATGGGCAGCTCGCACTTGCTCAA
  CAAGGGCCTGCCGCTTGTAAGACTTTGACCCCGACGCAGGCTTCAGTGATTGCCGCGGAT
  CAGAAGCCTGAGCACTTTGGCTCGAGACAATTAAGGACGTGGGATGAGGCTCCGAGACAG
  GACGCGGTTCTGCCTGGGGATCCTGAAGATAAAAAGCTTTGAAAAGTCGAATTCATGGTC
  GTGGAAGCTGAGCCCATATTAAGAGATGTCAGGCGTCCGACCTCCGATCATGAACGGGCC
  CCTGCACCCGCGGCCCCTGGTGGCATTGCTGGATGGCCGGGACTGCACAGTGGAGATGCC
  CATCCTGAAGGACGTGGCCACTGTGGCCTTCTGCGACGCGCAGTCCACGCAGGAGATCCA
  TGAGAAGGTCCTGAACGAGGCTGTGGGGGCCCTGATGTACCACACCATCACTCTCACCAG
  GGAGGACCTGGAGAAGTTCAAAGCCCTCCGCATCATCGTCCGGATTGGCAGTGGTTTTGA
  CAACATCGACATCAAGTCGGCCGGGGATTTAGGCATTGCCGTCTGCAACGTGCCCGCGGC
  GTCTGTGGAGGAGACGGCCGACTCGACGCTGTGCCACATCCTGAACCTGTACCGGCGGGC
  CACCTGGCTGCACCAGGCGCTGCGGGAGGGCACACGAGTCCAGAGCGTCGAGCAGATCCG
  CGAGGTGGCGTCCGGCGCTGCCAGGATCCGCGGGGAGACCTTGGGCATCATCGGACTTGG
  TCGCGTGGGGCAGGCAGTGGCGCTGCGGGCCAAGGCCTTCGGCTTCAACGTGCTCTTCTA
  CGACCCTTACTTGTCGGATGGCGTGGAGCGGGCGCTGGGGCTGCAGCGTGTCAGCACCCT
  GCAGGACCTGCTCTTCCACAGCGACTGCGTGACCCTGCACTGCGGCCTCAACGAGCACAA
  CCACCACCTCATCAACGACTTCACCGTCAAGCAGATGAGACAAGGGGCCTTCCTGGTGAA
  CACAGCCCGGGGTGGCCTGGTGGATGAGAAGGCGCTGGCCCAGGCCCTGAAGGAGGGCCG
  GATCCGCGGCGCGGCCCTGGATGTGCACGAGTCGGAACCCTTCAGCTTTAGCCAGGGCCC
  TCTGAAGGATGCACCCAACCTCATCTGCACCCCCCATGCTGCATGGTACAGCGAGCAGGC
  ATCCATCGAGATGCGAGAGGAGGCGGCACGGGAGATCCGCAGAGCCATCACAGGCCGGAT
  CCCAGACAGCCTGAAGAACTGTGTCAACAAGGACCATCTGACAGCCGCCACCCACTGGGC
  CAGCATGGACCCCGCCGTCGTGCACCCTGAGCTCAATGGGGCTGCCTATAGGTACCCTCC
  GGGCGTGGTGGGCGTGGCCCCCACTGGCATCCCAGCTGCTGTGGAAGGTATCGTCCCCAG
  CGCCATGTCCCTGTCCCACGGCCTGCCCCCTGTGGCCCACCCGCCCCACGCCCCTTCTCC
  TGGCCAAACCGTCAAGCCCGAGGCGGATAGAGACCACGCCAGTGACCAGTTGTAGCCCGG
  GAGGAGCTCTCCAGCCTCGGCGCCTGGGCAGAGGGCCCGGAAACCCTCGGACCAGAGTGT
  GTGGAGGAGGCATCTGTGTGGTGGCCCTGGCACTGCAGAGACTGGTCCGGGCTGTCAGGA
  GGCGGGAGGGGGCAGCGCTGGGCCTCGTGTCGCTTGTCGTCGTCCGTCCTGTGGGCGCTC
  TGCCCTGTGTCCTTCGCGTTCCTCGTTAAGCAGAAGAAGTCAGTAGTTATTCTCCCATGA
  ACGTTCTTGTCTGTGTACAGTTTTTAGAACATTACAAAGGATCTGTTTGCTTAGCTGTCA
  ACAAAAAGAAAACCTGAAGGAGCATTTGGAAGTCAATTTGAGGTTTTTTTTTTTGGTTTT
  TTTTTTTTTGTATGTTGGAACGTGCCCCAGAATGAGGCAGTTGGCAAACTTCTCAGGACA
  ATGAATCCTTCCCGTTTTTCTTTTTATGCCACACAGTGCATTGTTTTTTCTACCTGCTTG
  TCTTATTTTTAGAATAATTTAGAAAAACAAAACAAAGGCTGTTTTTCCTAATTTTGGCAT
  GAACCCCCCCTTGTTCCAAATGAAGACGGCATCACGAAGCAGCTCCAAAAGGAAAAGCTT
  GGGCGGTGCCCAGCGTGCCCGCTGCCCATCGACGTCTGTCCTGGGGACGTGGAGGGTGGC
  AGCGTCCCCGCCTGCACCAGTGCCGTCCTGCTGATGTGGTAGGCTAGCAATATTTTGGTT
  AAAATCATGTTTGTGACTGTAACCATTTGTATGAATTATTTTAAAGAAATAAAAATCCTG
  GAAAGAGAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001012614 unedited
  NNNCCGTACACCATTTGTAATACGACTCCACTATAGGCGGCCGCCATTCTGGTCGTGGAA
  GCTGAGCCCATATTAAGAGATGTCAGGCGTCCGACCTCCGATCATGAACGGGCCCCTGCA
  CCCGCGGCCCCTGGTGGCATTGCTGGATGGCCGGGACTGCACAGTGGAGATGCCCATCCT
  GAAGGACGTGGCCACTGTGGCCTTCTGCGACGCGCAGTCCACGCAGGAGATCCATGAGAA
  GGTCCTGAACGAGGCTGTGGGGGCCCTGATGTACCACACCATCACTCTCACCAGGGAGGA
  CCTGGAGAAGTTCAAAGCCCTCCGCATCATCGTCCGGATTGGCAGTGGTTTTGACAACAT
  CGACATCAAGTCGGCCGGGGATTTAGGCATTGCCGTCTGCAACGTGCCCGCGGCGTCTGT
  GGAGGAGACGGCCGACTCGACGCTGTGCCACATCCTGAACCTGTACCGGCGGGCCACCTG
  GCTGCACCAGGCGCTGCGGGAGGGCACACGAGTCCAGAGCGTCGAGCAGATCCGCGAGGT
  GGCGTCCGGCGCTGCCAGGATCCGCGGGGAGACCTTGGGCATCATCGGACTTGGTCGCGT
  GGGGCANGCAGTGGCGCTGCGGGCCAAGGCCTTCGGCTTCAACGTGCTCTTCTACACCCT
  TANCTTGTCGATGGCGTGGAGCGGGCGCTGGGGCTGCAGCGTGTCAGCACCCTGCANGAC
  CTGCTCTTCCCAGCGACTGCGTGACCCTGCACTGCGGCCTCAACGAGCACAACCACCACC
  TCATCAACGACTTCACCCGTAAGCAGATGAGACAGGGGGCCCTTCCTGGTGACACCGNCC
  CGGGGTGGCCCTGGTGGATGAGAAGGCGGCTGGCCCCGGCCCTGNAGGAAGGCCCCGATC
  CCCCGCCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_001012614
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012614.1, NP_001012632.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 1290 bp
• Locus ID: 1487
• Cytogenetics: 4p16.3
• Protein Pathways: Chronic myeloid leukemia, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
• Gene Summary: 'This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) has an alternate in-frame exon in the 5' coding region which results in translation initiation at a downstream ATG, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1.