gamma Adducin (ADD3) (NM_016824) Human Untagged Clone

CAT#: SC126647

ADD3 (untagged)-Human adducin 3 (gamma) (ADD3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD3
• Synonyms: ADDL; CPSQ3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016824, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGAT
  ATTTTGACCGCATCAATGAAAATGACCCAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACA
  AGACTTCAACATGATGGAGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGAC
  TTGGAATGCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGCAGA
  TTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTCTTGGCATGGTCAC
  ACCTATCAATGACCTTCCTGGTGCAGATACATCCTCATATGTGAAGGGAGAAAAACTTACTCGCTGTAAA
  CTTGCCAGCCTGTACAGACTTGTAGACTTGTTTGGATGGGCACACCTGGCAAATACCTATATCTCAGTAA
  GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAAGCTACAGCCTC
  CAATTTGGTGAAAGTCAATATAATAGGAGAAGTGGTTGACCAGGGAAGTACCAATTTGAAAATTGACCAT
  ACAGGATTCAGTCCCCATGCTGCAATCTATTCAACACGTCCTGATGTTAAGTGTGTGATACACATCCATA
  CCCTTGCAACAGCAGCTGTATCCTCCATGAAATGTGGGATCCTTCCAATTTCTCAAGAGTCTCTTCTTCT
  GGGAGATGTTGCCTATTATGACTACCAAGGGTCACTTGAAGAACAGGAGGAGAGAATTCAACTGCAGAAG
  GTTCTGGGACCAAGTTGTAAGGTGCTGGTACTCAGGAATCATGGTGTGGTTGCACTTGGAGAAACATTAG
  AGGAGGCTTTTCATTATATTTTTAATGTGCAACTAGCCTGTGAGATTCAGGTGCAGGCCCTAGCAGGTGC
  AGGTGGAGTAGACAATCTCCATGTACTGGACTTTCAGAAGTATAAAGCTTTCACTTACACTGTAGCAGCG
  TCTGGTGGAGGAGGTGTGAATATGGGTTCCCATCAAAAATGGAAGGTTGGCGAAATTGAGTTTGAAGGGC
  TTATGAGGACTCTGGACAACTTGGGGTATAGAACAGGCTATGCTTACAGGCATCCTCTCATTCGAGAGAA
  GCCTAGGCACAAGAGTGATGTGGAAATCCCAGCAACTGTGACTGCTTTTTCCTTTGAAGACGATACAGTG
  CCACTCTCTCCTCTCAAATACATGGCACAGAGGCAACAGCGTGAAAAAACAAGATGGCTGAACTCACCAA
  ATACTTACATGAAAGTGAATGTGCCTGAGGAGTCTCGGAACGGAGAAACCAGTCCCCGAACCAAAATCAC
  GTGGATGAAAGCAGAAGACTCATCTAAAGTTAGTGGTGGAACACCTATCAAAATTGAAGATCCAAATCAG
  TTTGTTCCTTTAAACACAAACCCGAATGAGGTACTAGAAAAGAGAAATAAGATTCGGGAACAAAATCGAT
  ATGACTTGAAAACAGCAGGACCACAATCTCAGTTGCTTGCTGGAATTGTTGTGGATAAGCCACCTTCTAC
  TATGCAATTTGAAGATGATGATCATGGCCCACCAGCTCCTCCTAACCCATTTAGTCATCTCACAGAAGGA
  GAACTTGAAGAGTATAAGAGGACAATCGAACGTAAACAACAAGGCCTAGAAGATGCTGAGCAGGAATTAC
  TCTCAGATGACGCTTCATCTGTTTCACAAATTCAGTCTCAAACTCAGTCACCGCAAAATGTCCCTGAAAA
  ATTAGAAGAAAACCATGAGCTGTTTTCCAAGAGCTTCATCTCCATGGAAGTGCCTGTCATGGTAGTAAAT
  GGCAAGGATGATATGCATGATGTTGAAGATGAGCTTGCTAAGCGAGTGAGTAGGTTAAGCACAAGTACAA
  CCATAGAAAACATCGAGATTACTATTAAGTCTCCAGAGAAAATCGAAGAAGTCCTGTCACCTGAAGGCTC
  CCCTTCAAAATCGCCATCCAAGAAAAAGAAGAAATTCCGCACTCCTTCTTTTCTGAAAAAGAACAAAAAA
  AAGGAGAAAGTTGAGGCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016824 unedited
  TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGCCGTAACG
  GTCGCCCAGTGTGAGGGGGCGGGNNAGGGAAAGAAGAGGGGTTTAAATTAGATTTTTTAA
  AACACAGAGCAAGCGCCAGAGGCGTCGGCATCCCAGGTGTCGCCGCTTCCTGCTGCACAG
  GGCTCGGCGTACAGGTCCCTCCCTCCTCAAGCCCCCTCCCCTTCTCCCGCCCTACCCTCT
  GGGGCTCTGCGGCGCTTAAGAGGCGGCCGCAGCGGCGGATCCGGCGGCTGCTGCAGCCCG
  GGCGGCTGCCGAGAAGGAGGGAGGGGAAACACAAAGCCGGCTACGCGCTGCGAGATAACA
  AGAGTAATCCACAGACTTAAAACATGAGCTCAGATGCCAGCCAAGGCGTGATTACCACTC
  CTCCTCCTCCCAGCATGCCTCACAAAGAGAGATATTTTGACCGCATCAATGAAAATGACC
  CAGAATACATTAGGGAGAGGAACATGTCTCCTGATCTACGACAAGACTTCAACATGATGG
  AGCAGAGGAAACGAGTTACTCAGATCCTGCAAAGTCCTGCCTTTCGGGAAGACTTGGAAT
  GCCTTATTCAAGAACAGATGAAGAAAGGCCACAACCCAACTGGATTACTAGCATTACAGC
  AGATTGCAGATTACATCATGGCCAATTCTTTCTCGGGTTTTTCTTCACCTCCTCTCAGTA
  AGAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTATCTTTTTCTGAA
  GCTACAGCCTNCAATTTGGNTGAAAGTCAATATATAGGGAAAAGTGGTTGACCCGGGAAG
  TACCCAATTGAAAAATGACCATACAGGATTCAGTCCCCATGCTGCAATCTATTCACACGT
  NCTGATGTTAAAGTGTGTGATACACATCCATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016824 unedited
  NNTTTTAGCTTGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCCAG
  AGTTAAACTAAATAACCCATGTTATGTGTACAAAATCCCCTTTGTTGAAAAATAAGGGGC
  TTTCTAAACTAATAAAAAAGGAAGTTTTCAAAAATTATAGTTTATTAAAACAACTTTTTT
  GGCAAACAAAGTTACTTCAGGTGAGGAAATTTTATACTGTGAATAAAATTCCAAATGAAT
  CTTTTCTTAAAACTTTTTAAAAAATTATGTGCCAGTGTATACTAATGCTATAGATTCTTG
  TCTTAGAAGTTTTTAAAGCATTCTGTTAATGCCCACTGAAACAATGGGACTCCAAAAATA
  TAGTCAATAATCATGATAAAAAATTATAATATGATTATCAAGTGAAGCAGGTATTGAGAA
  ATAAAAATTCTCACTTGCTCACTGGCAATTTCTTTCTAACAGATATTATGGAGAAGGCCT
  GAAGTAATTCAGACAGATAGCTGTTTATGGTGAATTATAATAATACTTCATGAGGGCAGA
  GCTAATTAAAACTAGTAATTGCTTAAAATTCAAAGCCATTTCTGGACATATAAAATGAGA
  GATGAATCTGAAAGTTTTTCTTTTGTAAAACCTCTTTCCAGTTCTTAAAGTCCAGTTTGC
  TACATCCCCCAATCTGATCTACCATTGCATATTAATGATCAACTTAAATGTGGTATTTAG
  ATGTGCAATGTCACATTGTTATAATAATAATTATAAAAAGACTCTATTTAGGCCTCAACT
  TTCTCCTTTTTTTTGGTCCTTTTTCAGAAAAGAAGGAGTGCGGAATTTCTTCTTTTTCTT
  GGATGCCGATTTTGAAGGGNAGCCTTCACGTGACAGGACTCCTTCGATTNTCTCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016824
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016824.3, NP_058432.1
• RefSeq Size: 4454 bp
• RefSeq ORF: 2121 bp
• Locus ID: 120
• Cytogenetics: 10q25.1-q25.2
• Domains: Aldolase_II
• Gene Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 4-6 encode the same isoform (a).