CRMP2 (DPYSL2) (NM_001386) Human Untagged Clone

CAT#: SC126729

DPYSL2 (untagged)-Human dihydropyrimidinase-like 2 (DPYSL2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPYSL2
• Synonyms: CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126729 sequence for NM_001386 edited (data generated by NextGen Sequencing)
  ATGTCTTATCAGGGGAAGAAAAATATTCCACGCATCACGAGCGATCGTCTTCTGATCAAA
  GGAGGTAAAATTGTTAATGATGACCAGTCGTTCTATGCAGACATATACATGGAAGATGGG
  TTGATCAAGCAAATAGGAGAAAATCTGATTGTGCCAGGAGGAGTGAAGACCATCGAGGCC
  CACTCCCGGATGGTGATCCCCGGAGGAATTGACGTCCACACTCGTTTCCAGATGCCTGAT
  CAGGGAATGACGTCTGCTGATGATTTCTTCCAAGGAACCAAGGCGGCCCTGGCTGGGGGA
  ACCACTATGATCATTGACCACGTTGTTCCTGAGCCTGGGACAAGCCTGCTCGCTGCCTTT
  GACCAGTGGAGGGAATGGGCCGACAGCAAGTCCTGCTGTGACTACTCTCTGCATGTGGAC
  ATCAGCGAGTGGCATAAGGGCATCCAGGAGGAGATGGAAGCGCTTGTGAAGGATCACGGG
  GTAAATTCCTTCCTCGTGTACATGGCTTTCAAAGATCGCTTCCAGCTAACGGATTGCCAG
  ATTTATGAAGTACTGAGTGTGATCCGGGATATTGGCGCCATAGCCCAAGTCCACGCAGAA
  AATGGCGACATCATTGCAGAGGAGCAGCAGAGGATCCTGGATCTGGGCATCACGGGCCCC
  GAGGGACATGTGCTGAGCCGACCTGAGGAGGTCGAGGCCGAAGCCGTGAATCGTGCCATC
  ACCATCGCCAACCAGACCAACTGCCCGCTGTATATCACCAAGGTGATGAGCAAAAGCTCT
  GCTGAGGTCATCGCCCAGGCACGGAAGAAGGGAACTGTGGTGTATGGCGAGCCCATCACT
  GCCAGCTTGGGAACGGACGGCTCCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCTGCC
  TTTGTCACCTCCCCACCCTTGAGCCCTGATCCAACCACTCCAGACTTTCTCAACTCCTTG
  CTGTCCTGTGGAGACCTCCAGGTCACGGGCAGTGCCCATTGCACGTTTAACACTGCCCAG
  AAGGCTGTAGGAAAGGACAACTTCACCCTGATTCCGGAGGGCACCAATGGCACTGAGGAG
  CGGATGTCCGTCATCTGGGACAAGGCTGTGGTCACTGGGAAGATGGATGAGAACCAGTTT
  GTGGCTGTGACCAGCACCAATGCAGCCAAAGTCTTCAACCTTTACCCCCGGAAAGGCCGC
  ATTGCTGTGGGATCCGATGCCGACCTGGTCATCTGGGACCCCGACAGCGTTAAAACCATC
  TCTGCCAAGACACACAACAGCTCTCTCGAGTACAACATCTTTGAAGGCATGGAGTGCCGC
  GGCTCCCCACTGGTGGTCATCAGCCAGGGGAAGATTGTCCTGGAGGACGGCACCCTGCAT
  GTCACCGAAGGCTCTGGACGCTACATTCCCCGGAAGCCCTTCCCTGATTTTGTTTACAAG
  CGTATCAAGGCAAGGAGCAGGCTGGCTGAGCTGAGAGGGGTTCCTCGTGGCCTGTATGAC
  GGACCTGTGTGTGAAGTGTCTGTGACGCCCAAGACAGTCACTCCAGCCTCCTCGGCCAAG
  ACGTCTCCTGCCAAGCAGCAGGCCCCACCTGTCCGGAACCTGCACCAGTCTGGATTCAGT
  TTGTCTGGTGCTCAGATTGATGACAACATTCCCCGCCGCACCACCCAGCGTATCGTGGCG
  CCCCCCGGTGGCCGTGCCAACATCACCAGCCTGGGCTAG
  
  Clone variation with respect to NM_001386.5
  426 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001386 unedited
  TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCTGTTTCTCTCTCTC
  CTTCTCTCTCTCTCTCTCTCTCTCTTTTTTTTCCGCCCTAGCTGGGGCTGTGTTGGAGGA
  GAGGAAGAAAGAGAGACAGAGGATTGCATTCATCCGTTACGTTCTTGAAATTTCCTAATA
  GCAAGACCAGCGAAGCGGTTGCACCCTTTTCAATCTTGCAAAGGAAAAAAACAAAACAAA
  ACAAAAAAAACCCAAGTCCCCTTCCCGGCAGTTTTTGCCTTAAAGCTGCCCTCTTGAAAT
  TAATTTTTTCCCAGGAGAGAGATGTCTTATCAGGGGAAGAAAAATATTCCACGCATCACG
  AGCGATCGTCTTCTGATCAAAGGAGGTAAAATTGTTAATGATGACCAGTCGTTCTATGCA
  GACATATACATGGAAGATGGGTTGATCAAGCAAATAGGAGAAAATCTGATTGTGCCAGGA
  GGAGTGAAGACCATCGAGGCCCACTCCCGGATGGTGATCCCCGGAGGAATTGACGTCCAC
  ACTCGTTTCCAGATGCCTGATCAGGGAATGACGTCTGCTGATGATTTCTTCCAAGGAACC
  AAGGCGGCCCTGGCTGGGGGACCACTATGATCATTGACCACGTTGNTCCTGAGCCTGGNG
  ACAGCCTGCTCGCTGCCTTTGACCAGTGGAGGGAATGGGCCGACAGCAAGTCCTGCTGTG
  ACTACTCNTCTGCATGTGGACATCAAGCGAGTGGCATAAGGGCATNNCAGAGGAGATGGA
  ANNCGCTGTGGAGGATCCACGGGGTAATTCTTCCCTCGGGACATGGCTTTACAAGATCGC
  TTCACTAACGGGATGCCAGATTATGAGNCCTGGGGGTGGATCCGGGGATATGGGGCCCCT
  ACCCCAGTTCCCGCAAAAATGGGGACTTCTTTGCAAGAGCGCAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001386 unedited
  TAGCTTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTGAAACAACCACATTTTGCTTTATTAGGGGTTCCA
  TGGGAATATAACACAGAGTTTTTAAGGAATAATAAACCCGAAACTTGGTTTTTACCATAA
  TTATTTTGCCATCAAAACAGGGGTTACAAAAAAAAAAGTACTTTTTTGGCTACCCACTTT
  ATCAGCTTTAAAACTGAAAGTTCCTCCACTCATGGGGCACAATCACAATTTCCAGGATTA
  AAAACCATTTATTTTTAAATCCTACACTGGTTTAAAGCATCAAGGCACTGGAGTTATTCA
  GTTCTGAAACACTGGCGATTTATTTTAACATTTACATATGACATTCTTTTAACAGACCCC
  CAAAGCAAGCCAACAGGGAAACATGCTTACACAGCCTGGAAAAATCGGCAGGGTTTAACT
  TGTTTTTTAAAAAAACAACCCAAACAACCAAAAATTACCCTGACCCGGGTCAGGAAAAAC
  AGGAAGACTCAAGGGAATACTAGAGCTGCGAGGGTTTCTTAAAATTGAAACAAAAATTGT
  TTTTTTCCAACTTGTTCAAATTTCCTCTAAGGGCAGGGGAGAAAAAAAGCAAAATTTTTA
  GGCACCCCATTTTTTTTTTTAAAAGGGCAATTCCCTTTTAAAAACCCAATAAACCACACC
  CCCCCAAAACCCCAAACCCCACCCGATTCCCCAAGGCAAAAGGTTAAAAAACAGGGTGGG
  GGAGGGAAGACCTACTTTGGGAGGTTTTCACCTTTCAAAACCAAAATGTGGTTAAAAAAA
  GCCGGAAAACACAATTGTTTAAAAAACTGAACACCGTTGGAAAAATGGGCACCAATCACA
  GGGCGGGGGTTGGCCCAAGGCCCCTTGGGGGAAGGGGCCAAAAGGAGGGGGGTGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001386
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001386.4, NP_001377.1
• RefSeq Size: 4567 bp
• RefSeq ORF: 1719 bp
• Locus ID: 1808
• Cytogenetics: 8p21.2
• Domains: Amidohydro_1
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.