MANEA (NM_024641) Human Untagged Clone

CAT#: SC126732

MANEA (untagged)-Human mannosidase, endo-alpha (MANEA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MANEA
• Synonyms: ENDO; hEndo
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126732 sequence for NM_024641 edited (data generated by NextGen Sequencing)
  ATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTCTATTTATTTTC
  TCTCTGATGATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTGGAGCTCCTTTT
  GGACTTGACCTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGAAAAATTTTGAT
  TTCCAAAAGAGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAAAAAGTGTTGAA
  ATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTACCACCTCTGAAC
  AATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTGATGGTAAATAT
  ATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCAAGAATTATCCA
  CAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTGAATTGGGAAGT
  TACAGTTCTCGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGCGCTCAGCTTCA
  ATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAAATGGAGAACCT
  ACTGATAACTTGGTACCCACTATTTTGGATAAAGCTCATAAATATAACCTAAAGGTTACT
  TTTCACATAGAACCATATAGCAATCGAGATGATCAAAACATGTACAAAAATGTCAAGTAT
  ATTATAGACAAATATGGAAATCATCCGGCCTTTTACAGGTACAAGACGAAGACTGGCAAT
  GCTCTTCCTATGTTTTATGTCTATGATTCCTATATTACCAAGCCTGAAAAATGGGCCAAT
  CTGTTAACCACCTCAGGGTCTCGGAGTATTCGCAATTCTCCTTATGATGGACTGTTTATT
  GCCCTTCTGGTAGAAGAAAAACATAAGTATGATATTCTTCAAAGTGGTTTTGATGGAATT
  TACACATATTTTGCCACAAATGGCTTTACTTATGGCTCATCACATCAGAATTGGGCTAGC
  CTAAAATTATTTTGTGATAAATACAACTTAATATTTATCCCAAGTGTGGGCCCAGGATAC
  ATAGATACCAGCATCCGTCCATGGAACACGCAAAACACTCGGAACCGAATCAATGGGAAG
  TATTATGAAATTGGTCTGAGTGCCGCACTTCAGACACGCCCCAGCTTAATTTCTATCACC
  TCTTTTAATGAGTGGCATGAAGGAACTCAGATTGAAAAAGCTGTTCCCAAAAGAACCAGT
  AATACAGTGTACCTAGATTACCGTCCTCATAAACCAGGTCTTTACCTAGAACTGACTCGC
  AAGTGGTCTGAAAAATACAGTAAGGAAAGAGCAACTTATGCATTAGATCGCCAGCTGCCT
  GTTTCTTAA
  
  Clone variation with respect to NM_024641.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024641 unedited
  TCACTATAGGCGGCCCGCGAATTCGCACGAGGGTTCGCGGTCTTAACCTCTCCTCTGGCC
  GAGTCCTTGCAAGAAGTGAATTACCCGACCCTGCAAAACACTTACTATTTTGGAGTTTAA
  AGTATGTCATCATGGCAAAGTTTCGGAGAAGGACTTGCATCATTTTGGCACTTTTTATTC
  TATTTATTTTCTCTCTGATGATGGGTTTAAAAATGCTGAGACCAAATACAGCTACTTTTG
  GAGCTCCTTTTGGACTTGACCTTCTTCCAGAACTTCATCAACGAACTATTCATTTGGGGA
  AAAATTTTGATTTCCAAAAGAGTGACAGAATCAACAGTGAAACAAATACCAAGAATTTAA
  AAAGTGTTGAAATCACTATGAAACCTTCCAAAGCCTCTGAACTTAACTTGGATGAACTAC
  CACCTCTGAACAATTATCTACATGTATTTTATTACAGTTGGTATGGAAATCCACAATTTG
  ATGGTAAATATATACATTGGAATCATCCAGTGTTAGAGCATTGGGACCCTAGAATAGCCA
  AGAATTATCCACAAGGGAGACACAACCCTCCAGATGACATTGGCTCCAGCTTTTATCCTG
  AATTGGGAAGTTACAGTTCTCGGGATCCTTCTGTCATAGAAACTCACATGAGACAAATGC
  GCTCAGCTTCAATTGGTGTACTAGCCCTCTCTTGGTACCCACCTGATGTAAATGATGAAA
  ATGGAGACCTACTGATAACTTGGTACCCACTATTNTGGATAAGCTCATAATATACCCTAA
  GTTACTTTTACATANAACATATAGCATCGAGATGATCAAACTGTCAAAATGTCAGTTTTT
  ATAGACAATATGAAATATCCGGCCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024641 unedited
  ATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  ATTTCATCTATGTTTTTATTAGGTAAAAATTAACCATTCCATATAACAAAATAGTAATTT
  TTAATTACAATCTTAAAACAGAAGGAAGTTAAACAACTCAAGGGAAGTTAATAACAGCAG
  TTTTTGACAATATTAGTCCTTAAAAGAAACAGTAAAATATTTGGTTTCTATAACAGTCAA
  AACAATTAGACTGATTAAACCATAAAAATTAATAACTTATAAAAGAATCCATTATGATTC
  ATGCATTGTATGTATTATACCACTTTAAGTTATACCATATTGAATTCAAACGTATAGTTT
  TGAATTAAGAAATAAATTCTTTGCTCTTATTTACTTGTTATCATAACAATTTAAACTGGG
  TTACAACTAAAATGGTAAAGGCAGTTGTAATAAATACCTGAGGAAGATTTTGAAAGTTTT
  CCTCTCTAAGAATTAACAGAATTATTAGAGTTATTTCCTAATGCAGTTGGCTCACCTTAT
  CTGTAGGTTCTACATCCATGGCCAAGAAATTGCCGACTGAAACTCTGAAGATGTACAGAC
  TCTTTTTTTTCATGTAATTATTCACTAACCAGTATGGTATAACAACTACATAGCATTTAC
  ATTGTATTAAGTGTTATAAATAATCTAGAGATGACTTAATGTATACAGGAGGATGTGCAT
  AGATTATATGCAACTACTATGCCAGTTTATATCAGGGACTTGAGCATCTGCAGATTTTGG
  TATCTATAGGGGTTACTGGNACCCATCCNCCACAGACACTGGGGATGACTGNAATTATTT
  TATTAAAAGGGGNAGAAAAACTATATAATTAAACTAGTACTATGAGATCGATGCCATNCT
  TCANGAGATAACTTTTGCATGAGCACGAACTGCTTACCTACTCGAAACTGTACTGCTGCT
  GTTAGGNTATGGGTGAGGACCCACAAACTAGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024641
• ORF Size: 1389 bp
• Insert Size: 4530
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_024641.2, NP_078917.2
• RefSeq Size: 4593
• RefSeq ORF: 1389
• Locus ID: 79694
• Protein Families: Transmembrane
• Gene Summary: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]). [supplied by OMIM, Sep 2008]