DHPS (NM_013407) Human Untagged Clone

CAT#: SC126747

DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHPS
• Synonyms: DHS; MIG13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013407, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAGCTCGACGT
  TGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGA
  GGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATC
  GAGAAGAAGCTGGAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCAC
  TTACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCG
  CTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC
  AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAGAACGGGATCA
  ATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAGGACTGGCTGATGCCCATTCT
  GGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTG
  GGCAAGGAGATCAACAACCCAGAGTCCGTGTATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTC
  CCGCACTTACAGACGGCTCGCTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCT
  GGACATCGTTGAGGGTGCCCGACCAGACGAGGCTGTCTCCTGGGGCAAGATCCGGGTGGATGCACAGCCC
  GTCAAGGTCTATGCTGACGCCTCCCTGGTCTTCCCCCTGCTTGTGGCTGAAACCTTTGCCCAGAAGATGG
  ATGCCTTCATGCATGAGAAGAACGAGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013407 unedited
  GGCCGCGAAATTCGCACGAGGCTCTTGCGGAGACGCGCGCGTCGGGGTTTAACGCGTTTC
  TGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCGAGAGCCGGCTATAGGCGCAT
  GGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAG
  CTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAA
  TTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCG
  CGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTGTCACAGGATGA
  AGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGCACCATTTTCCT
  GGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGCTACCTTGTGCA
  GCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATCAA
  GTGCCTGGCGCCCACATACTTGNGGCGAGTTTAGCCTCAGGGGGGAAGGAGCTCCGGGAG
  AACGGGATCAATAGGATCGGGAACCTGGCTGGTGCCCAATGAGAATTACTGGCAGTTTTG
  AGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGACACAGNAGGTGTTAA
  AGTGGACGCCTCTTAGATGATCGCCCCGCTGGCCAAGAGATCACACCCAGAGTCCGGGTA
  TTACTGGGCCCAGAGACCACTTCCTGGGTTTAGTCCGCCTTACGACGGNTTGTTGGCAAT
  TGATCTTTTCCATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013407 unedited
  ACCGGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTGGGACTTCAAATACCATCTTATTTTAAAAACCTAACTGACCAAAAAATAAGGGAGGG
  GCTGGGTCTGCAAATTAATAAATAAAAAAGGGGGGAAAAACTTTCTGGGACCCCAACCGC
  TCAATCCTCGTTCTTTTCATGCCTGAAGGCATCCATTTTTTGGGCAAAGGTTTCAACCAC
  AAGCAGGGGGAAAACCAAGGAGGCGTCAACATAAACCTTGACGGGCTGTGCATCCACCCG
  GATCTTGCCCCAAGAAACAACCTCGTTTGGTCGGGCACCTGAGTCAAAACCCTCAAACTC
  CTGGGCTGGGTTGATGTAAACAACGTAATCGGCCCCCTTCCCCATGAGGGTGGCATTGGC
  AATGTGGGGCTTGACCACCCCCCCGCCCAAAATGATCATCCCAATGCACTTGGCAAAAAT
  GGCCTGTGTGTTGATGAACCTCAAGGCCTCAACGATGTCCAAGACCAAGCCCGGGTTCTT
  GTAGGAATGGAAAAAGATCATGTCGCCCAACGAGCCGTCTGTAAGTGCGGGACTAAACAC
  AGGGATGTGGGTCTTCTGGGCCCAATAATACACGGACTCTGGGTTGGTGATCTCCTTGCC
  CAACCGGGCGATCATCTTAAAAGGCGTCCACTTTACACCCTCTGTGGTCTGGTCCATCAC
  CATACTGGCCAAAATGGGGATTAACCCGTTCTCAAACTTGCAGTAATCTTATTGGGCACC
  ACCAAGTTTCCAACCTATGATCCCCGTCTCCCCGGACTCCTTTCCCTGAAGCTAACTCGC
  CCAATTTGGGGCGCCCAGCACTGATGAAGCTTTCCTCCCCCCCCACTGTGGGACCAATAC
  CTCCACATTTGGGCTCCAAAGGACCAAAGGGCTCCCGGATCCCTAACTGTGGAGGTGGAA
  GTATATTCCAAAAATGTGCACCTTGTAATGGCCGCGGCTTGGGCTAGCCCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013407
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013407.1, NP_037539.1
• RefSeq Size: 1183 bp
• RefSeq ORF: 942 bp
• Locus ID: 1725
• Cytogenetics: 19p13.13
• Domains: DS
• Gene Summary: 'This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]'
  Transcript Variant: This splice variant (3) lacks exon 7 and includes alternative exon 8a.