IRF8 (NM_002163) Human Untagged Clone

CAT#: SC126770

IRF8 (untagged)-Human interferon regulatory factor 8 (IRF8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF8
• Synonyms: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126770 sequence for NM_002163 edited (data generated by NextGen Sequencing)
  ATGTGTGACCGGAATGGTGGTCGGCGGCTTCGACAGTGGCTGATCGAGCAGATTGACAGT
  AGCATGTATCCAGGACTGATTTGGGAGAATGAGGAGAAGAGCATGTTCCGGATCCCTTGG
  AAACACGCTGGCAAGCAAGATTATAATCAGGAAGTGGATGCCTCCATTTTTAAGGCCTGG
  GCAGTTTTTAAAGGGAAGTTTAAAGAAGGGGACAAAGCTGAACCAGCCACTTGGAAGACG
  AGGTTACGCTGTGCTTTGAATAAGAGCCCAGATTTTGAGGAAGTGACGGACCGGTCCCAA
  CTGGACATTTCCGAGCCATACAAAGTTTACCGAATTGTTCCTGAGGAAGAGCAAAAATGC
  AAACTAGGCGTGGCAACTGCTGGCTGCGTGAATGAAGTTACAGAGATGGAGTGCGGTCGC
  TCTGAAATCGACGAGCTGATCAAGGAGCCTTCTGTGGACGATTACATGGGGATGATCAAA
  AGGAGCCCTTCCCCGCCGGAGGCCTGTCGGAGTCAGCTCCTTCCAGACTGGTGGGCGCAG
  CAGCCCAGCACAGGCGTGCCGCTGGTGACGGGGTACACCACCTACGACGCGCACCATTCA
  GCATTCTCCCAGATGGTGATCAGCTTCTACTATGGGGGCAAGCTGGTGGGCCAGGCCACC
  ACCACCTGCCCCGAGGGCTGCCGCCTGTCCCTGAGCCAGCCTGGGCTGCCCGGCACCAAG
  CTGTATGGGCCCGAGGGCCTGGAGCTGGTGCGCTTCCCGCCGGCCGACGCCATCCCCAGC
  GAGCGACAGAGGCAGGTGACGCGGAAGCTGTTCGGGCACCTGGAGCGCGGGGTGCTGCTG
  CACAGCAGCCGGCAGGGCGTGTTCGTCAAGCGGCTGTGCCAGGGCCGCGTGTTCTGCAGC
  GGCAACGCCGTGGTGTGCAAAGGCAGGCCCAACAAGCTGGAGCGTGATGAGGTGGTCCAG
  GTCTTCGACACCAGCCAGTTCTTCCGAGAGCTGCAGCAGTTCTATAACAGCCAGGGCCGG
  CTTCCTGACGGCAGGGTGGTGCTGTGCTTTGGGGAAGAGTTTCCGGATATGGCCCCCTTG
  CGCTCCAAACTCATTCTCGTGCAGATTGAGCAGCTGTATGTCCGGCAACTGGCAGAAGAG
  GCTGGGAAGAGCTGTGGAGCCGGCTCTGTGATGCAGGCCCCCGAGGAGCCGCCGCCAGAC
  CAGGTCTTCCGGATGTTTCCAGATATTTGTGCCTCACACCAGAGATCATTTTTCAGAGAA
  AACCAACAGATCACCGTCTAA
  
  Clone variation with respect to NM_002163.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002163 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCAGCAAGCGTGGGAACG
  CGGGCGGCGAGACGGCGGCAGGACGGCGGCAGGATGTGTGACCGGAATGGTGGTCGGCGG
  CTTCGACAGTGGCTGATCGAGCAGATTGACAGTAGCATGTATCCAGGACTGATTTGGGAG
  AATGAGGAGAAGAGCATGTTCCGGATCCCTTGGAAACACGCTGGCAAGCAAGATTATAAT
  CAGGAAGTGGATGCCTCCATTTTTAAGGCCTGGGCAGTTTTTAAAGGGAAGTTTAAAGAA
  GGGGACAAAGCTGAACCAGCCACTTGGAAGACGAGGTTACGCTGTGCTTTGAATAAGAGC
  CCAGATTTTGAGGAAGTGACGGACCGGTCCCAACTGGACATTTCCGAGCCATACAAAGTT
  TACCGAATTGTTCCTGAGGAAGAGCAAAAATGCAAACTAGGCGTGGCAACTGCTGGCTGC
  GTGAATGAAGTTACAGAGATGGAGTGCGGTCGCTCTGAAATCGACGAGCTGATCAAGGAG
  CCTTCTGTGGACGATTACATGGGGATGATCAAAAGGAGCCCTTCCCCGCCGGAGGCCTGT
  CGGAGTCAGCTCCTTCCAGACTGGTGGGCGCAGCAGCCCAGCACAGGCGTGCCGCTGGTG
  ACGGNGTACACCACCTACGACGCGCACCATTCAGCATTCTCCCAGATGGTGATCAGCTTC
  TACTATGGGGGCAAGCTGGTGGGCCANGCCACCACCACCTGCCCCGATGGCTGNCGNCTG
  TCCCTGAGCCAGCCTGGGCTGCCCNGCACCAATCTGTATGGGCCCGATGGCCTGNNAGCT
  GTGCGCTTNCCGNNCGGCGACGCATNCNCAGNGAGCGACAGAGGCAGTGACGCGNAAATC
  TGTCGGCACCTGGAGCGCGGGTGCTGCTGCACACNCCCGCAGGGCGTGTCGTCAGTCGCT
  GTGCAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002163 unedited
  TATGGACCGCGGCCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTAAAAAAGAGCA
  TTTTATTTTAATAAAGAATAAAAGAGATCAATATACTGTTTTAATGGATACAAAAATAAA
  TATTCATTCAGCATATTAAAGATATGTGCTTTGACATTCATTTGAATTGGAGATTCAAGC
  CTATTGTTATCTTATGAACACTTCAGCAAACAGGTCTGCCATTCTTAAAAATATAATGCT
  TTGTTGGACAAAAGGGACAAGCCACGTCCCCTGGTCCTCTCCTCTATTCGCCTGTGAACT
  CCATCCACACGTAAAGGACCTCTGGGTCTGACTGTCCCCTCCACAGGCATGGTGCTGGGA
  AAAGGAAACAGGCATATCTGGCTTTTCAGATTTTAAACCGGAAACTCTCACAGTCACAAA
  TCCACCATGAGACTTGGGAGATTGGATGAGCTGTCTCCCAAACCCTAACACCTTCCACCT
  TCTCAAAATGAAGGCTGCCCTTTCACTGGGAGGTTCTGAATGCGGGATTGGTGCTGACTC
  AGGCTGGGCACAAAGGAGAAAGGAGGACATGCGAAATCCCCCACTTCGAAATCCAAATAT
  TTCACACACATGTTGAAACCCATTTGGGAAAGCAAAAAAAGAGGTTTCCTGGCACTGATT
  TCACTGAACCGAAATTGAAACACAATAATTCTAGTTCCTAATCCCTCCTGGCCCCCAAAC
  CCCCAAAGGGAGAAGGTTCCTATTGGTTTGAGGATAAAAAACCACCCCCGCTCGGCCTGG
  TATTTACACCATATTCCCTGGCCCACAATTTCTTAAAAGAATTTTCCTTCTCTTTTCTCC
  AACTCCCAGAACTTTCTTCTTGCACTCCTCCAAAGGCGGCTTTCTATTCTAAATCTTTCC
  ACACCCCTTCTCTAACAGCCTCAACGTTCACAAACATTCTCCCTCCTACACCCACCCTCC
  N
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002163
• Insert Size: 2620 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002163.2, NP_002154.1
• RefSeq Size: 2678 bp
• RefSeq ORF: 1281 bp
• Locus ID: 3394
• Cytogenetics: 16q24.1
• Domains: IRF
• Protein Families: Transcription Factors
• Gene Summary: 'Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]'