AKR1B1 (NM_001628) Human Untagged Clone

CAT#: SC126774

AKR1B1 (untagged)-Human aldo-keto reductase family 1, member B1 (aldose reductase) (AKR1B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKR1B1
• Synonyms: ADR; ALDR1; ALR2; AR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126774 sequence for NM_001628 edited (data generated by NextGen Sequencing)
  ATGGCAAGCCGTCTCCTGCTCAACAACGGCGCCAAGATGCCCATCCTGGGGTTGGGTACC
  TGGAAGTCCCCTCCAGGGCAGGTGACTGAGGCCGTGAAGGTGGCCATTGACGTCGGGTAC
  CGCCACATCGACTGTGCCCATGTGTACCAGAATGAGAATGAGGTGGGGGTGGCCATTCAG
  GAGAAGCTCAGGGAGCAGGTGGTGAAGCGTGAGGAGCTCTTCATCGTCAGCAAGCTGTGG
  TGCACGTACCATGAGAAGGGCCTGGTGAAAGGAGCCTGCCAGAAGACACTCAGCGACCTG
  AAGCTGGACTACCTGGACCTCTACCTTATTCACTGGCCGACTGGCTTTAAGCCTGGGAAG
  GAATTTTTCCCATTGGATGAGTCGGGCAATGTGGTTCCCAGTGACACCAACATTCTGGAC
  ACGTGGGCGGCCATGGAAGAGCTGGTGGATGAAGGGCTGGTGAAAGCTATTGGCATCTCC
  AACTTCAACCATCTCCAGGTGGAGATGATCTTAAACAAACCTGGCTTGAAGTATAAGCCT
  GCAGTTAACCAGATTGAGTGCCACCCATATCTCACTCAGGAGAAGTTAATCCAGTACTGC
  CAGTCCAAAGGCATCGTGGTGACCGCCTACAGCCCCCTCGGCTCTCCTGACAGGCCCTGG
  GCCAAGCCCGAGGACCCTTCTCTCCTGGAGGATCCCAGGATCAAGGCGATCGCAGCCAAG
  CACAATAAAACTACAGCCCAGGTCCTGATCCGGTTCCCCATGCAGAGGAACTTGGTGGTG
  ATCCCCAAGTCTGTGACACCAGAACGCATTGCTGAGAACTTTAAGGTCTTTGACTTTGAA
  CTGAGCAGCCAGGATATGACCACCTTACTCAGCTACAACAGGAACTGGAGGGTCTGTGCC
  TTGTTGAGCTGTACCTCCCACAAGGATTACCCCTTCCATGAAGAGTTTTGA
  
  Clone variation with respect to NM_001628.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001628 unedited
  GAACACCATTACTATAGGCGGCCGCGCAATTCGCACGAGGCCTCGTGCCGAATTCGGCAC
  GAGGCACCGTACTGGGCGGGGGTCTGGGGAGCGCAGCAGCCATGGCAAGCCGTCTCCTGC
  TCAACAACGGCGCCAAGATGCCCATCCTGGGGTTGGGTACCTGGAAGTCCCCTCCAGGGC
  AGGTGACTGAGGCCGTGAAGGTGGCCATTGACGTCGGGTACCGCCACATCGACTGTGCCC
  ATGTGTACCAGAATGAGAATGAGGTGGGGGTGGCCATTCAGGAGAAGCTCAGGGAGCAGG
  TGGTGAAGCGTGAGGAGCTCTTCATCGTCAGCAAGCTGTGGTGCACGTACCATGAGAAGG
  GCCTGGTGAAAGGAGCCTGCCAGAAGACACTCAGCGACCTGAAGCTGGACTACCTGGACC
  TCTACCTTATTCACTGGCCGACTGGCTTTAAGCCTGGGAAGGAATTTTTCCCATTGGATG
  AGTCGGGCAATGTGGTTCCCAGTGACACCAACATTCTGGACACGTGGGCGGCCATGGAAG
  AGCTGGTGGATGAAGGGCTGGTGAAAGCTATTGGCATCTCCAACTTCAACCATCTCCAGG
  TGGAGATGATCTTAAACAAACCTGGCTTGAAGTATAAGCCTGCAGTTAACCAGATTGAGT
  GCCACCCATATCTCACTCAGGAGAAGTTAATCCAGTACTGCCAGTCCAAAGGCATCGTGG
  TGACCGCCTACAGCCCCCTCGGCTCTTCTGACAGGCCCTGNGCCAAGCCCCGAGACCCTT
  CTCTTNCTGGAGATCCCAGNATCAAGGCGATCGCAGCCAAGCACAATAAACTACANGCCC
  AGTCCTTGATCCGGNTCCCCATGCAGNAGAACTTGNTGGNTGACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001628 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACACAAAAGCACTTTT
  TATTTGAGGCAAAGAGAAGTCTTGCTGAAAGGATTCCAGTTCCAAGCAGTCAAAACTCAA
  CCGTTAGTGGCACTATTTTGACCTGGTAGATTTTGCTTCTCTTTGGTCAGAAAAGGGTAT
  TCAGGTTGTACTTTCCCCAGCAGGGTAGAAAGAAGGGCAAAGCAAACTGGAAGAGACTTC
  TACTCTACTGACAGGGCTCTTGAGATCCAACATCAAGCTAGACACGCCCTCGCTGGCCAC
  TCTACAGGTTGCTGTCCCACTGCTGAGTGACACAGGCCATACTACATTTGCAAGGAAAAA
  AATGAGGCAAGAAACACAGGTATAGGTCACTTGGGGACGAGCAGGCAACCACAGCTTCAA
  AACTCTTCATGGAAGGGGTAATCCTTGTGGGAGGTACAGCTCAACAAGGCACAGACCCTC
  CAGTTCCTGTTGTAGCTGAGTAAGGTGGTCATATCCTGGCTGCTCAGTTCAAAGTCAAAG
  ACCTTAAAGTTCTCAGCAATGCGTTCTGGTGTCACAGACTTGNGGATCACCACCAAGTTC
  CTCTGCATGGGGAACCGGATCAGGACCTGGGCTGTANGTTTATTGTGCTTGGCTGCGATC
  GCCTTGATCCTGGGATCCTCCAGGAGAAGAAGGTCCTCGGGCTTGGCCANGGCCTGTCAA
  GAAACCGAGGGGGCTGTAAGCGGCACCACGATGCTTTGGACTGGCAGACTGGATAACTCT
  CCTGATGAGAATGGGGGGACTAATCTGGTACTGCAGCTTATACTTAACCCAGTTTGTTAG
  ATCTCTCCCCTGAAAGGTGAATTGGAATCCCATCCTTTCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001628
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001628.2, NP_001619.1
• RefSeq Size: 1416 bp
• RefSeq ORF: 951 bp
• Locus ID: 231
• Cytogenetics: 7q33
• Domains: aldo_ket_red
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycerolipid metabolism, Metabolic pathways, Pentose and glucuronate interconversions, Pyruvate metabolism
• Gene Summary: 'This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]'
  Transcript Variant: This variant (1) encodes isoform 1.