EFEMP1 (NM_004105) Human Untagged Clone

CAT#: SC126895

EFEMP1 (untagged)-Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EFEMP1
• Synonyms: DHRD; DRAD; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; FBNL; fibrillin-like; fibulin 3; FLJ35535; MGC111353; MLVT; MTLV; S1-5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126895 sequence for NM_004105 edited (data generated by NextGen Sequencing)
  ATGTTGAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACC
  GAAGAAACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAG
  CAATGCAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAG
  TGTGTCAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAAT
  AATGAACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGG
  GTTGTAGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCC
  AGTGCTGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGG
  CGGAACCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCA
  GCAGGCTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCAGGG
  ACGCACAACTGTAGAGCAGACCAAGTGTGCATCAATTTACGGGGATCCTTTGCATGTCAG
  TGCCCTCCTGGATATCAGAAGCGAGGGGAGCAGTGCGTAGACATAGATGAATGTACCATC
  TCTCCATATTGCCACCAAAGATGCGTGAATACACCAGGCTCATTTTATTGCCAGTGCAGT
  CCTGGGTTTCAATTGGCAGCAAACAACTATACCTGCGTAGATATAAATGAATGTGATGCC
  AGCAATCAATGTGCTCAGCAGTGCTACAACATTCTTGGTTCATTCATCTGTCAGTGCAAT
  CAAGGATATGAGCTAAGCAGTGACAGGCTCAACTGTGAAGACATTGATGAATGCAGAACC
  TCAAGCTACCTGTGTCAATATCAATGTGTCAATGAACCTGGGAAATTCTCATGTATGTGC
  CCCCAGGGATACCAAGTGGTGAGAAGTAGAACATGTCAAGATATAAATGAGTGTGAGACC
  ACAAATGAATGCCGGGAGGATGAAATGTGTTGGAATTATCATGGCGGCTTCCGTTGTTAT
  CCACGAAATCCTTGTCAAGATCCCTACATTCTAACACCAGAGAACCGATGTGTTTGCCCA
  GTCTCAAATGCCATGTGCCGAGAACTGCCCCAGTCAATAGTCTACAAATACATGAGCATC
  CGATCTGATAGGTCTGTGCCATCAGACATCTTCCAGATACAGGCCACAACTATTTATGCC
  AACACCATCAATACTTTTCGGATTAAATCTGGAAATGAAAATGGAGAGTTCTACCTACGA
  CAAACAAGTCCTGTAAGTGCAATGCTTGTGCTCGTGAAGTCATTATCAGGACCAAGAGAA
  CATATCGTGGACCTGGAGATGCTGACAGTCAGCAGTATAGGGACCTTCCGCACAAGCTCT
  GTGTTAAGATTGACAATAATAGTGGGGCCATTTTCATTTTAG
  
  Clone variation with respect to NM_004105.3
  661 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004105 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGCCCTCCCCT
  GCGCACTCCCCTCGCTGCCCGGGCCCGGAGCGCAGCGCGGCCGCACAGATTCACAATGTT
  GAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACCGAAGA
  AACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAGCAATG
  CAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAGTGTGT
  CAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAATAATGA
  ACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGGGTTGT
  AGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCCAGTGC
  TGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGGCGGAA
  CCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCAGCAGG
  CTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCANGGACGCA
  CAACTGTAGAGCAGACCAAGTGTGCATCAATTACGGGGATCCTTTGCATGTCAGTGCCCT
  CCTGGATATCANAAGCGAGGGGAGCAGTGCGTAAACATAGATGAATGTACCATCTCTTCA
  TATTGCCACCAAAGATGCGTGAATACACCANGCTCATTTTATTTGCAGTGCAGTCCTGGG
  TTTCAATTGGCAGCAACAACTATACCTGCGTAGATATAATGAATGGGATGCCAGCAATCA
  TGTGCTCAGCAGTGCTACAACATTCTTGTTCATCATCTGTCATGCAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004105 unedited
  TGACCGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTCTAAAGAAATTATCTTT
  ATTGACCTTTTCCATTTTCTTACAAAGCAGTTAAAAACTCATTCTTACCCTTGCATGCTA
  TTCAATGGTTATGATGGCTGCCTCCTTATGAGACTGTTAGTGGAGCTCTAGTAAGTTTCC
  TTAAGCACCACAGAAGATCATCATTGCTTGGTCCCACTTTTATAGGAAAGAATCCAAGTT
  TCACCAGATAGTGTATACTTAGCTCTCTTGAAGAAGACCAGTTTAGTGGATTAATGTCTA
  ATTTACATATAGTAATAAAGACAAACTTTGAATCTTTACATATTAAATGCCCACTTTATA
  CCATGGTGTAATTGTTTGAATTATGGGTGAGTGTACAGTATAGAGATGTAGATGCACTAG
  ATATATCTATAAATAAAATAGGGCTTTAAGGTAACAATATTCTTTGGCTGACTTAAATGC
  CTGTGGTTGACTCTTAGAAAAGACTAAAATGAAAATGGCCCCACTATTATTGTCAATCTT
  AACACAGAGCTTGTGCGGAAGGTCCCTATACTGCTGACTGTCAGCATCTCCAGGTCCACG
  ATATGTTCTCTTGGTCCTGATAATGACTTCACGAGCACAAGCATTGCACTTACAGGACTT
  GTTTGTCGTAGGTAGAACTCTCCATTTTCATTTCCAGATTTAATCCCGAAAGTATTGATG
  GGTGTGGCATAAATAGTTGTAGCCCTGTATCTGGAAAATGTCTGATGCACANACCTATCA
  AATCGGATGCTCATGTATTTGTANACTATTGACTGGGGCAGTTCTGGCACATGGCATTTG
  AGACTGGGCAAACACATCGGTCCTTTGGGGTAAAATGTAGGGATCTGACAGGNATTTCCT
  GGGTAACACCNAAGCCGCCTGGAATTTCCACCATTTATTCTCCCCGCGTTCCTTGGGGGC
  AAACCCATATTTATTGGATGTGTTCCCTTCACCCCTGGGNTNCCGGGGGGCCCTCTGGAA
  TATTCACGGTCTTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004105
• Insert Size: 2050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004105.2, NP_004096.2
• RefSeq Size: 2742 bp
• RefSeq ORF: 1482 bp
• Locus ID: 2202
• Cytogenetics: 2p16.1
• Domains: EGF_CA, EGF, EGF
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.