Aconitase 1 (ACO1) (NM_002197) Human Untagged Clone

CAT#: SC126974

ACO1 (untagged)-Human aconitase 1, soluble (ACO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACO1
• Synonyms: ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126974 sequence for NM_002197 edited (data generated by NextGen Sequencing)
  ATGAGCAACCCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAA
  TTCTTCAATTTGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGA
  GTTCTTCTGGAAGCAGCCATTCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATT
  GAAAATATTCTACATTGGAATGTCACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCT
  GCTCGTGTCATCCTGCAGGACTTTACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATG
  CGTGATGCTGTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCT
  GATCTTGTAATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAG
  AAGAATCAAGACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGT
  TCCCAGGCTTTTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTG
  AATTTGGAATATTTGGCAAGAGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGC
  CTCGTGGGCACAGACTCGCACACTACCATGATTGATGGCTTGGGCATTCTTGGTTGGGGT
  GTCGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGCCAATCAGTATGGTGCTTCCT
  CAGGTGATTGGCTACAGGCTGATGGGGAAGCCCCACCCTCTGGTAACATCCACTGACATC
  GTGCTCACCATTACCAAGCACCTCCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTC
  TTCGGGCCTGGAGTAGCCCAGTTGTCCATTGCTGACCGAGCTACGATTGCTAACATGTGT
  CCAGAGTACGGAGCAACTGCTGCCTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTG
  GTGCAAACAGGTCGTGATGAAGAAAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTA
  GGAATGTTTCGAGATTTCAATGACCCTTCTCAAGACCCAGACTTCACCCAGGTTGTGGAA
  TTAGATTTGAAAACAGTAGTGCCTTGCTGTAGTGGACCCAAAAGGCCTCAGGACAAAGTT
  GCTGTGTCCGACATGAAAAAGGACTTTGAGAGCTGCCTTGGAGCCAAGCAAGGATTTAAA
  GGATTCCAAGTTGCTCCTGAACATCATAATGACCATAAGACCTTTATCTATGATAACACT
  GAGTTCACCCTTGCTCATGGTTCTGTGGTCATTGCTGCCATTACTAGCTGCACAAACACC
  AGTAATCCGTCTGTGATGTTAGGGGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGC
  CTGAACGTGATGCCTTACATCAAAACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTAC
  TACCTACAAGAAAGCGGAGTCATGCCTTATCTGTCTCAGCTTGGGTTTGACGTGGTGGGC
  TATGGCTGCATGACCTGCATTGGCAACAGTGGGCCTTTACCTGAACCTGTGGTAGAAGCC
  ATCACACAGGGAGACCTTGTAGCTGTTGGAGTACTATCTGGAAACAGGAATTTTGAAGGT
  CGAGTTCACCCCAACACCCGGGCCAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATAT
  GCAATTGCTGGAACCATCAGAATCGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAG
  GGACAGCAGGTATTTCTGAAAGATATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAG
  CGTCAGTATGTCATCCCGGGGATGTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAAT
  GAAAGCTGGAATGCCTTAGCAACCCCATCAGATAAGCTGTTTTTCTGGAATTCCAAATCT
  ACGTATATCAAATCACCACCATTCTTTGAAAACCTGACTTTGGATCTTCAGCCCCCTAAA
  TCTATAGTGGATGCCTATGTGCTGCTAAATTTGGGAGATTCGGTAACAACTGACCACATC
  TCCCCAGCTGGAAATATTGCAAGAAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGC
  CTAACTCCACGAGAATTCAACTCCTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCA
  CGGGGAACATTTGCCAACATTCGCTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAG
  ACTATCCATCTGCCTTCTGGGGAAATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAG
  CAGGCAGGCCTTCCCCTGATCGTTCTGGCTGGCAAAGAGTACGGTGCAGGCAGCTCCCGA
  GACTGGGCAGCTAAGGGCCCTTTCCTGCTGGGAATCAAAGCCGTCCTGGCCGAGAGCTAC
  GAGCGCATTCACCGCAGTAACCTGGTTGGGATGGGTGTGATCCCACTTGAATATCTCCCT
  GGTGAGAATGCAGATGCCCTGGGGCTCACAGGGCAAGAACGATACACTATCATTATTCCA
  GAAAACCTCAAACCACAAATGAAAGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAG
  GCTGTCATGAGGTTTGACACTGATGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTC
  AACTACATGATCCGCAAGATGGCCAAGTAG
  
  Clone variation with respect to NM_002197.2
  1263 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002197 unedited
  GGCCGCGAATTCGGCACCAGCGCACGGGAACGCGTCCCGCTGCTTGGGTCAGGTTCGCCG
  GTCGCGGGAGCCCCGCCGTGCAGTCGGAGGAACACGTGGCCATCAGTAATCATGAGCAAC
  CCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAAT
  TTGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTG
  GAAGCAGCCATTCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATT
  CTACATTGGAATGTCACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTC
  ATCCTGCAGGACTTTACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATGCGTGATGCT
  GTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCTGATCTTGTA
  ATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAGAATCAA
  GACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCT
  TTTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAA
  TATTTGGNCAGAGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGC
  ACAGACTCGCACACTACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002197 unedited
  CGCATTTGTCTGAANCGCGGCCCGCAAACTACCAGTCGAGCTTTTTTTTTTTTTTTTTTT
  TTCATGAAAAAGATTCATTTCATTCAAATCAGAAATGTGTTCTCCCAAACCGCCTCCCAC
  AAAGGTCTCTGCTTGATCAATGAAAAGATAGTAAAAGAAACCTTCACATTTCTTTTTATG
  TGGATCTATTAAAGGGTACATAATCAAAACAACTTTAACACCCGACCTATCCATGAGGAG
  TCTGGATGATTCACTCTGACGGAAAAGGGGAGAGAAGAGAGAATCACTGCTCTGATTCGA
  AGCGTGCAACGTTCTTATCTATGCCACTCACCACTGGTATACTTAATACTGACGGGAAAC
  TGACCTTCAAAAGGAAGGCTTCATTGACCAGACCGTAACAGGAACAATACGAGGGCAGGA
  GACACCTCCTGAGAAGGATTGCTTGCGCTCAGCTGACCATTACTCGGAACCCACTAACAC
  CCCATCGTCCTGCGACCACTCACTTTAACAGACCCCTTGGCTTCTATCTTTCTGCCAATA
  CTGCCATTCCCCCCCTTCTACCCTCCACATTCTGCACACCGACATGACGCCTACCACCCC
  TTCCGACTGCACTACCCTCCTGCCTCCGTTCTCCACGGATTGGCACCAAACCCTCTGGGC
  TCACTTCTTTTTTTTACCGGACCAACCCCTACCACAAGAACTCGCTGAGGACTTTTCAAC
  CGGGATTTGTAATACCTGTAGCATCCGCCTACCTCCTTTGCACCCCACCATCACAACTCA
  CCATCCCCCTACACACCCCATCTATCTACCACACACTGCCACAATTAACCTTTCCCCACG
  TTCCACCTAAATATCCGCCCATCCCACATACACATATCCCCTCCTCTACTTCCATCCCTA
  CTCTCACGCGCTCTATCACAACTCACAACCACATGCTCATCGGCCCACTTTACCTCAACN
  ACCACCTACCGCCTCTTACCTACAATAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002197
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002197.1, NP_002188.1
• RefSeq Size: 3498 bp
• RefSeq ORF: 2670 bp
• Locus ID: 48
• Cytogenetics: 9p21.1
• Domains: Aconitase_C, aconitase
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.