Aconitase 1 (ACO1) (NM_002197) Human Untagged Clone

CAT#: SC322429

ACO1 (untagged)-Human aconitase 1, soluble (ACO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACO1
• Synonyms: ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322429 GCCGTGCAGTCGGAGGAACACGTGGCCATCAGTAATCATGAGCAACCCATTCGCACACCT
  TGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAATTTGAATAAATTGGA
  GGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTGGAAGCAGCCATTCG
  GAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATTCTACATTGGAATGT
  CACGCAGCACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTCATCCTGCAGGACTT
  TACGGGTGTGCCCGCTGTGGTTGACTTTGCTGCAATGCGTGATGCTGTGAAAAAGTTAGG
  AGGAGATCCAGAGAAAATAAACCCTGTCTGCCCTGCTGATCTTGTAATAGATCATTCCAT
  CCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAGAATCAAGACCTGGAATTTGA
  AAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCTTTTCACAACATGCG
  GATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAATATTTGGCAAGAGT
  GGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGCACAGACTCGCACAC
  TACCATGATTGATGGCTTGGGCATTCTTGGTTGGGGTGTCGGTGGTATTGAAGCAGAAGC
  TGTCATGCTGGGTCAGCCAATCAGTATGGTGCTTCCTCAGGTGATTGGCTACAGGCTGAT
  GGGGAAGCCCCACCCTCTGGTAACATCCACTGACATCGTGCTCACCATTACCAAGCACCT
  CCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTCTTCGGGCCTGGAGTAGCCCAGTT
  GTCCATTGCTGACCGAGCTACGATTGCTAACATGTGTCCAGAGTACGGAGCAACTGCTGC
  CTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTGGTGCAAACAGGTCGTGATGAAGA
  AAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTAGGAATGTTTCGAGATTTCAATGA
  CCCTTCTCAAGACCCAGACTTCACCCAGGTTGTGGAATTAGATTTGAAAACAGTAGTGCC
  TTGCTGTAGTGGACCCAAAAGGCCTCAGGACAAAGTTGCTGTGTCCGACATGAAAAAGGA
  CTTTGAGAGCTGCCTTGGAGCCAAGCAAGGATTTAAAGGATTCCAAGTTGCTCCTGAACA
  TCATAATGACCATAAGACCTTTATCTATGATAACACTGAATTCACCCTTGCTCATGGTTC
  TGTGGTCATTGCTGCCATTACTAGCTGCACAAACACCAGTAATCCGTCTGTGATGTTAGG
  GGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGCCTGAACGTGATGCCTTACATCAA
  AACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTACTACCTACAAGAAAGCGGAGTCAT
  GCCTTATCTGTCTCAGCTTGGGTTTGACGTGGTGGGCTATGGCTGCATGACCTGCATTGG
  CAACAGTGGGCCTTTACCTGAACCTGTGGTAGAAGCCATCACACAGGGAGACCTTGTAGC
  TGTTGGAGTACTATCTGGAAACAGGAATTTTGAAGGTCGAGTTCACCCCAACACCCGGGC
  CAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATATGCAATTGCTGGAACCATCAGAAT
  CGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAGGGACAGCAGGTATTTCTGAAAGA
  TATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAGCGTCAGTATGTCATCCCGGGGAT
  GTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAATGAAAGCTGGAATGCCTTAGCAAC
  CCCATCAGATAAGCTGTTTTTCTGGAATTCCAAATCTACGTATATCAAATCACCACCATT
  CTTTGAAAACCTGACTTTGGATCTTCAGCCCCCTAAATCTATAGTGGATGCCTATGTGCT
  GCTAAATTTGGGAGATTCGGTAACAACTGACCACATCTCCCCAGCTGGAAATATTGCAAG
  AAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGCCTAACTCCACGAGAATTCAACTC
  CTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCACGGGGAACATTTGCCAACATTCG
  CTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAGACTATCCATCTGCCTTCTGGGGA
  AATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAGCAGGCAGGCCTTCCCCTGATCGT
  TCTGGCTGGCAAAGAGTACGGTGCAGGCAGCTCCCGAGACTGGGCAGCTAAGGGCCCTTT
  CCTGCTGGGAATCAAAGCCGTCCTGGCCGAGAGCTACGAGCGCATTCACCGCAGTAACCT
  GGTTGGGATGGGTGTGATCCCACTTGAATATCTCCCTGGTGAGAATGCAGATGCCCTGGG
  GCTCACAGGGCAAGAACGATACACTATCATTATTCCAGAAAACCTCAAACCACAAATGAA
  AGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAGGCTGTCATGAGGTTTGACACTGA
  TGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTCAACTACATGATCCGCAAGATGGC
  CAAGTAGGAGACGTGCACTTGGTGCTGCGCCCAGGGAGGAAGCCGCACCACCAGCCAGCG
  CAGGCCCTGGTGGAGAGGCCTCCCTGGCTGCCTCTGGGAGGGGTGCTGCCTTGTAGATGG
  AGCAAGTGAGCACTGAGGGTCTGGTGCCAATCCTGTAGGCACAAAACCAGAAGTTTCTAC
  ATTCTCTATTTTTGTTAATCATCTTCTCTTTTTCCAGAATTTGGAAGCTAGAATGGTGGG
  AATGTCAGTAGTGCCAGAAAGAGAGAACCAAGCTTGTCTTTAAAGTTACTGATCACAGGA
  CGTTGCTTTTTCACTGTTTCCTATTAATCTTCAGCTGAACACAAGCAAACCTTCTCAGGA
  GGTGTCTCCTACCCTCTTATTGTTCCTCTTACGCTCTGCTCAATGAAACCTTCCTCTTGA
  GGGTCATTTTCCTTTCTGTATTAATTATACCAGTGTTAAGTGACATAGATAAGAACTTTG
  CACACTTCAAATCAGAGCAGTGATTCTCTCTTCTCTCCCCTTTTCCTTCAGAGTGAATCA
  TCCAGACTCCTCATGGATAGGTCGGGTGTTAAAGTTGTTTTGATTATGTACCTTTTGATA
  GATCCACATAAAAAGAAATGTGAAGTTTTCTTTTACTATCTTTTCATTTATCAAGCAGAG
  ACCTTTGTTGGGAGGCGGTTTGGGAGAACACATTTCTAATTTGAATGAAATGAAATCTAT
  TTTCAGTGAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002197
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002197.1, NP_002188.1
• RefSeq Size: 3498 bp
• RefSeq ORF: 2670 bp
• Locus ID: 48
• Cytogenetics: 9p21.1
• Domains: Aconitase_C, aconitase
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.