PHGDH (NM_006623) Human Untagged Clone

CAT#: SC127007

PHGDH (untagged)-Human phosphoglycerate dehydrogenase (PHGDH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHGDH
• Synonyms: 3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127007 sequence for NM_006623 edited (data generated by NextGen Sequencing)
  ATGGCTTTTGCAAATCTGCGGAAAGTGCTCATCAGTGACAGCCTGGACCCTTGCTGCCGG
  AAGATCTTGCAAGATGGAGGGCTGCAGGTGGTGGAAAAGCAGAACCTTAGCAAAGAGGAG
  CTGATAGCGGAGCTGCAGGACTGTGAAGGCCTTATTGTTCGCTCTGCCACCAAGGTGACC
  GCTGATGTCATCAACGCAGCTGAGAAACTCCAGGTGGTGGGCAGGGCTGGCACAGGTGTG
  GACAATGTGGATCTGGAGGCCGCAACAAGGAAGGGCATCTTGGTTATGAACACCCCCAAT
  GGGAACAGCCTCAGTGCCGCAGAACTCACTTGTGGAATGATCATGTGCCTGGCCAGGCAG
  ATTCCCCAGGCGACGGCTTCGATGAAGGACGGCAAATGGGAGCGGAAGAAGTTCATGGGA
  ACAGAGCTGAATGGAAAGACCCTGGGAATTCTTGGCCTGGGCAGGATTGGGAGAGAGGTA
  GCTACCCGGATGCAGTCCTTTGGGATGAAGACTATAGGGTATGACCCCATCATTTCCCCA
  GAGGTCTCGGCCTCCTTTGGTGTTCAGCAGCTGCCCCTGGAGGAGATCTGGCCTCTCTGT
  GATTTCATCACTGTGCACACTCCTCTCCTGCCCTCCACGACAGGCTTGCTGAATGACAAC
  ACCTTTGCCCAGTGCAAGAAGGGGGTGCGTGTGGTGAACTGTGCCCGTGGAGGGATCGTG
  GACGAAGGCGCCCTGCTCCGGGCCCTGCAGTCTGGCCAGTGTGCCGGGGCTGCACTGGAC
  GTGTTTACGGAAGAGCCGCCACGGGACCGGGCCTTGGTGGACCATGAGAATGTCATCAGC
  TGTCCCCACCTGGGTGCCAGCACCAAGGAGGCTCAGAGCCGCTGTGGGGAGGAAATTGCT
  GTTCAGTTCGTGGACATGGTGAAGGGGAAATCTCTCACGGGGGTTGTGAATGCCCAGGCC
  CTTACCAGTGCCTTCTCTCCACACACCAAGCCTTGGATTGGTCTGGCAGAAGCTCTGGGG
  ACACTGATGCGAGCCTGGGCTGGGTCCCCCAAAGGGACCATCCAGGTGATAACACAGGGA
  ACATCCCTGAAGAATGCTGGGAACTGCCTAAGCCCCGCAGTCATTGTCGGCCTCCTGAAA
  GAGGCTTCCAAGCAGGCGGATGTGAACTTGGTGAACGCTAAGCTGCTGGTGAAAGAGGCT
  GGCCTCAATGTCACCACCTCCCACAGCCCTGCTGCACCAGGGGAGCAAGGCTTCGGGGAA
  TGCCTCCTGGCCGTGGCCCTGGCAGGCGCCCCTTACCAGGCTGTGGGCTTGGTCCAAGGC
  ACTACACCTGTACTGCAGGGGCTCAATGGAGCTGTCTTCAGGCCAGAAGTGCCTCTCCGC
  AGGGACCTGCCCCTGCTCCTATTCCGGACTCAGACCTCTGACCCTGCAATGCTGCCTACC
  ATGATTGGCCTCCTGGCAGAGGCAGGCGTGCGGCTGCTGTCCTACCAGACTTCACTGGTG
  TCAGATGGGGAGACCTGGCACGTCATGGGCATCTCCTCCTTGCTGCCCAGCCTGGAAGCG
  TGGAAGCAGCATGTGACTGAAGCCTTCCAGTTCCACTTCTAA
  
  Clone variation with respect to NM_006623.3
  1326 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006623 unedited
  AGCCTACGATTCACTATAGGGACGGCCGCGGNGCCGAACCGCAGCTTCTTGGCTTAGGTA
  CTTCTACNACAGCGGCCGATTCCGAGGCCAACTCCAGCAATGGCTTTTGCAAATCTGCGG
  AAAGTGCTCATCAGTGACAGCCTGGACCCTTGCTGCCGGAAGATCTTGCAAGATGGAGGG
  CTGCAGGTGGTGGAAAAGCAGAACCTTATCAAAGAGGAGCTGATAGCGGAGCTGCAGGAC
  TGTGAAGGCCTTATTGTTCGCTCTGCCACCAAGGTGACCGCTGATGTCATCAACGCAGCT
  GAGAAACTCCAGGTGGTGGGCAGGGCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCC
  GCAACAAGGAAGGGCATCTTGGTTATGAACACCCCCAATGGGAACAGCCTCAGTGCCGCA
  GAACTCACTTGTGGAATGATCATGTGCCTGGCCAGGCAGATTCCCCAGGCGACGGCTTCG
  ATGAAGGACGGCAAATGGGAGCGGAAGAAGTTCATGGGAACAGAGCTGAATGGAAAGACC
  CTGGGAATTCTTGGCCTGNGCAGGATTGGGAGAGAGGTAGCTACCCGGATGCAGTCCTTT
  GGGATGAAGACTATAGGGTATGACCCCATCATTTCCCCAGAGGTCTCGGCCTCCTTTGGT
  GTTCAGCAGCTGCCCCTGGAGGAGATCTGGCCTCTCTGTGATTTCATCACTGTGCACACT
  CCTCTCCTGCCCTCCACGACAGGCTTGCTGAATGACAACACCTTTGCCCAGTGCAAGATG
  GGGTGCGTGTGGTGAACTGTGCCCGTGGAGGGATCGTGGACGAAGCGCCCTGCTCCGGCC
  CTGCAGTCTGCCAGTGTGCCGNGCTGACTGGACGTGNTTACGAAGAGCCGCACGGACCGG
  CCNNTGTGACATGAGATGTATCACTGTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006623 unedited
  ATCGCTGCCGCAATTTANAGTCTAGTTATTAATCTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTATTGTTGTTTTTTTTTTTTTTTGGGGGGAGGCTTTTTATTAAACGGG
  TATTGGTGTACTACAGGGTCAAAGTGCAATGTAAACAGTGTCAAAAGCCCCCGTTCAACC
  CAAAAATGGGGATTTTCTCTCCCTATTGATCACAGGGGGGGGGTTTTTTTAAAAAAACCC
  CAAAAGCAGGGACCAATGAGCTCCAAGGTTAAAAATGGAACTGGAAGGGTTTAATCACAT
  GCTGGTTTCACGCTTTCAAGCTGGGCAACAAGGAGGAAATGCCCATGACGTGCCAAGTTT
  TCCCATTTGACACCAGTGAAATCTGGTAGGACAAGAGCCGCACGCCTGGCTTTGGCAGGA
  GGCCAATTATGGGAAGCAACATTGCAGGGTGAAAAGTCTTAATCCCGAATAAGAACAAGG
  GCCAGTCCCTGCGGAAAGGGCCTTTTTGGCTGAAAACAGCTTCCTTGAACCCCTGTAGAA
  CAAGTGTAGGGGCTTGGACCAAACCCCCAACCTGGTAAAGGGGCGCCTGCCAAGGCCACC
  GCCAAGAGGGATTCTCCCAAAGCCTTTTTTCCCTGGGGCAAAAGGGTTTTGGGAAGTGGT
  GACAATTAAGGCCGCTTCTTTTAACACCAGCTTAACGTTTACAAGATTTACAATCCCCTT
  GGTTGAGAACCCTTTTTCAGGAGGGCCGACAAATACTTGTGGGGGGTTATAAAAGTTCCC
  AATATTTTTAAGGGGTGTCCCCGGGGTATAACCCGGGAGGGGCCCCTTTGGGGGAACCCA
  CCCCAACGCTCGCATAATTGTTCCCCAAGAATTTGTGGCCACAACCATCATAAGGCTTTG
  TGTTTTGGAGAAAAAGAAACTTTGATAGGCCTCTGGATATTACAACACCCCCCGAGAAGA
  ATTTCCCCTTCTCCAGTTCCCACACATAGACACAGAAATTTCTCCACCACAAGGTGCTTG
  AGCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006623
• ORF Size: 1602 bp
• Insert Size: 2000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_006623.2, NP_006614.2
• RefSeq Size: 1968
• RefSeq ORF: 1602
• Locus ID: 26227
• Domains: 2-Hacid_DH, 2-Hacid_DH_C
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]